Delayed harvest of short stature corn plants

ABSTRACT

Methods for delayed harvesting of corn fields are provided herein. These methods provide an extended, flexible period of time to harvest corn. The methods allow growers to harvest their corn at the optimal time for drying down or accessing seed, without increasing the risk of losing yield to lodging.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims benefit of U.S. Provisional Application No.63/117,231, filed Nov. 23, 2020; U.S. Provisional Application No.63/117,237, filed Nov. 23, 2020; U.S. Provisional Application No.63/117,247, filed Nov. 23, 2020; U.S. Provisional Application No.63/117,225, filed Nov. 23, 2020; and U.S. Provisional Application No.63/125,752, filed Dec. 15, 2020; and U.S. Provisional Application No.63/180,344, filed Apr. 27, 2021, all of which are incorporated byreference in their entireties herein.

INCORPORATION OF SEQUENCE LISTING

The instant application contains a Sequence Listing which has beensubmitted electronically in ASCII format and is hereby incorporated byreference in its entirety. Said ASCII copy, created on Nov. 19, 2021, isnamed P35026US01_SL.txt and is 1,161,026 bytes in size.

FIELD

The present disclosure relates to methods of delayed harvest of cornfields.

BACKGROUND

Corn can be harvested after fertilization, grain fill and maturity, buttypically after drying down to a desired moisture content for storage.Growers have to balance product value, plant health, kernel moisturecontent, and standability (e.g., due to the propensity of corn to lodge)of corn plants when determining the optimum time for harvesting. If agrower harvests corn before it reaches its optimal kernel moisturecontent, the grower may have to use artificial drying methods to furtherreduce the kernel moisture content before storage. Conversely, if agrower waits to harvest corn (or cannot harvest due to physical weatherbarriers such as rain or snow) until it reaches or passes optimal kernelmoisture, then the longer the amount of time the crop remains in thefield, the greater the risk of lodging from weather events (e.g., strongwinds) and/or plant senescence (i.e., deterioration from age). Thus,there is a need for farmers to have greater flexibility to leave cornplants in the field for later harvest to allow for greater access and/ordry down of kernels.

SUMMARY

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a DNAsegment inserted into the endogenous br2 locus, wherein the DNA segmentencodes an antisense RNA that is at least 70% complementary to at least20 consecutive nucleotides of SEQ ID NO: 132 or 180, and wherein themutant allele of the endogenous br2 locus produces an RNA transcriptcomprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a deletionof at least one nucleotide from an endogenous br2 locus as compared toSEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a premature stop codon within anucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous br2 locus, wherein the DNAsegment encodes an antisense RNA that is at least 70% complementary toat least 20 consecutive nucleotides of SEQ ID NO: 132 or 180, andwherein the mutant allele of the endogenous br2 locus produces an RNAtranscript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises adeletion of at least one nucleotide from an endogenous br2 locus ascompared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a premature stop codon withina nucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a DNAsegment inserted into the endogenous br2 locus, wherein the DNA segmentencodes an antisense RNA that is at least 70% complementary to at least20 consecutive nucleotides of SEQ ID NO: 132 or 180, and wherein themutant allele of the endogenous br2 locus produces an RNA transcriptcomprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a deletionof at least one nucleotide from an endogenous br2 locus as compared toSEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a premature stop codon within anucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous br2 locus, wherein the DNAsegment encodes an antisense RNA that is at least 70% complementary toat least 20 consecutive nucleotides of SEQ ID NO: 132 or 180, andwherein the mutant allele of the endogenous br2 locus produces an RNAtranscript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises adeletion of at least one nucleotide from an endogenous br2 locus ascompared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a dominant orsemi-dominant transgene or mutant allele of a gene, and wherein thetransgene or mutant allele causes a short stature phenotype in the atleast one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a premature stop codonwithin a nucleic acid sequence encoding a Brachytic2 protein as comparedto a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a recombinant DNA construct comprising a transcribable DNAsequence encoding a GA2 oxidase protein and a plant-expressiblepromoter, wherein the transcribable DNA sequence is operably linked tothe plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a recombinant DNA construct comprising a transcribable DNAsequence encoding a GA2 oxidase protein and a plant-expressiblepromoter, wherein the transcribable DNA sequence is operably linked tothe plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a recombinant DNA construct comprising a transcribable DNAsequence encoding a GA2 oxidase protein and a plant-expressiblepromoter, wherein the transcribable DNA sequence is operably linked tothe plant-expressible promoter.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 depicts the stalk health ratings of short and tall corn plants atnormal and late harvest times.

FIG. 2 depicts an example of a planting, maturation, and harvestingschedule for corn.

DETAILED DESCRIPTION

Unless defined otherwise, all technical and scientific terms used havethe same meaning as commonly understood by one of ordinary skill in theart to which this disclosure belongs. Unless otherwise provided, where aterm is provided in the singular, this disclosure also contemplates theplural of that term. Where there are discrepancies in terms anddefinitions used in references that are incorporated by reference, theterms used in this application shall have the definitions given herein.Other technical terms used have their ordinary meaning in the art inwhich they are used, as exemplified by various art-specificdictionaries, for example, “The American Heritage® Science Dictionary”(Editors of the American Heritage Dictionaries, 2011, Houghton MifflinHarcourt, Boston and New York), the “McGraw-Hill Dictionary ofScientific and Technical Terms” (6th edition, 2002, McGraw-Hill, NewYork), or the “Oxford Dictionary of Biology” (6th edition, 2008, OxfordUniversity Press, Oxford and New York). Any references cited herein,including, e.g., all patents, published patent applications, andnon-patent publications, are incorporated herein by reference in theirentirety.

When a grouping of alternatives is presented, any and all combinationsof the members that make up that grouping of alternatives isspecifically envisioned. For example, if an item is selected from agroup consisting of A, B, C, and D, the inventors specifically envisioneach alternative individually (e.g., A alone, B alone, etc.), as well ascombinations such as A, B, and D; A, C, and D; A, B, and C; A and C; Band C; A and B; etc. The term “and/or” when used in a list of two ormore items means any one of the listed items by itself or in combinationwith any one or more of the other listed items. For example, theexpression “A and/or B” is intended to mean either or both of A andB—i.e., A alone, B alone, or A and B in combination. The expression “A,B and/or C” is intended to mean A alone, B alone, C alone, A and B incombination, A and C in combination, B and C in combination, or A, B,and C in combination.

As well understood in the art, metric measurement values provided hereincan be easily converted to standard (S.I.) units where relevant, andvice versa.

As used herein, a “plant” includes an explant, plant part, seedling,plantlet or whole plant at any stage of regeneration or development. Ascommonly understood, a “corn plant” or “maize plant” refers to any plantof species Zea mays and includes all plant varieties that can be bredwith corn, including wild maize species.

As used herein, a “plant part” can refer to any organ or intact tissueof a plant, such as a meristem, shoot organ/structure (e.g., leaf, stemor node), root, flower or floral organ/structure (e.g., bract, sepal,petal, stamen, carpel, anther and ovule), seed, embryo, endosperm, seedcoat, fruit, the mature ovary, propagule, or other plant tissues (e.g.,vascular tissue, dermal tissue, ground tissue, and the like), or anyportion thereof. Plant parts of the present disclosure can be viable,nonviable, regenerable, and/or non-regenerable. A “propagule” caninclude any plant part that can grow into an entire plant.

As used herein, a “locus” is a chromosomal locus or region where apolymorphic nucleic acid, trait determinant, gene, or marker is located.A “locus” can be shared by two homologous chromosomes to refer to theircorresponding locus or region. Without being limiting, a locus cancomprise a polynucleotide that encodes a protein or an RNA. A locus canalso comprise a non-coding RNA. A locus can comprise a gene. A locus cancomprise a promoter, a 5′-untranslated region (UTR), an exon, an intron,a 3′-UTR, or any combination thereof. A locus can comprise a codingregion.

As used herein, an “allele” refers to an alternative (e.g., variant)nucleic acid sequence of a gene or at a particular locus (e.g., anucleic acid sequence of a gene or locus that is different than otheralleles for the same gene or locus). Such an allele can be considered(i) wild-type or (ii) mutant if one or more mutations or edits arepresent in the nucleic acid sequence of the mutant allele relative tothe wild-type allele. A mutant or edited allele for a gene may have areduced or eliminated activity or expression level for the gene relativeto the wild-type allele. According to present embodiments, a mutant oredited allele for a gene may have an inversion or antisense sequencethat may be complementary to another portion of the gene and/or a codingsequence of another copy or allele of the gene and/or another gene. Fordiploid organisms such as corn, a first allele can occur on onechromosome, and a second allele can occur at the same locus on a secondhomologous chromosome. If one allele at a locus on one chromosome of aplant is a mutant or edited allele and the other corresponding allele onthe homologous chromosome of the plant is wild-type, then the plant isdescribed as being heterozygous for the mutant or edited allele.However, if both alleles at a locus are mutant or edited alleles, thenthe plant is described as being homozygous for the mutant or editedalleles. A plant homozygous for mutant alleles at a locus may comprisethe same mutant or edited allele or different mutant or edited allelesif heteroallelic or biallelic.

As used herein, an “endogenous locus” refers to a locus at its naturaland original chromosomal location. As used herein, the “endogenous GA20oxidase_3 locus” refers to the GA20 oxidase_3 genic locus at itsoriginal chromosomal location. As used herein, the “endogenous GA20oxidase_5 locus” refers to the GA20 oxidase_5 genic locus at itsoriginal chromosomal location. As used herein, the “endogenous GA3oxidase locus” refers to the GA3 oxidase genic locus at its originalchromosomal location. As used herein, the “endogenous br2 locus” refersto the br2 genic locus at its original chromosomal location.

As used herein, a “female” corn plant refers to a corn plant thatcomprises one or more female reproductive structures that are capable ofproducing corn ear(s) and kernels (seed). In an aspect, a corn plantprovided herein is a female corn plant. In an aspect, a female cornplant is male sterile. In another aspect, a female corn plant isdetasseled. In an aspect, the male reproductive organs or flowers (e.g.,tassels) of a female corn plant are chemically sterilized (e.g., byapplication of an herbicide to plants lacking tolerance to the herbicidein those male reproductive organs, flowers or tassels), such as with aRoundup® Hybridization System (RHS). In another aspect, the malereproductive organs or flowers (e.g., tassels) of a female corn plantare sterilized due to cytoplasmic male sterility (or CMS). It isappreciated in the art that a corn plant is monoecious and can beconsidered both a male corn plant and a female corn plant. In an aspect,a female corn plant is capable of producing pollen. For purposes of thepresent disclosure, a corn plant having one or more male reproductiveorgan or structure, such as tassels, and/or capable of producing pollen,is considered a “female” plant if used to generate a corn ear(s) and/orcorn seed (i.e., kernels) for production and harvest. A corn plantlacking a male reproductive organ or structure, such as tassels, havinga sterilized male reproductive organ or structure, and/or incapable ofproducing pollen, is also considered a “female” plant if used togenerate a corn ear(s) and/or seed (i.e., kernels) for production andharvest. A “female” corn plant may include any pollen-receiving cornplant that produces an ear or female reproductive organ, which canreceive pollen from a pollen-bearing corn plant.

As used herein, a “male” corn plant refers to a corn plant that iscapable of producing pollen (e.g., form one or more tassels) and is usedto pollinate and/or fertilize one or more female corn plant(s) for seedproduction and harvest, even if the male plant further has a femalereproductive structure(s) that is/are capable of producing a corn earand kernels (seed), which may or may not be harvested. A “male” cornplant may include any pollen-bearing (or pollen-producing) corn plant,which can provide its pollen to a pollen-receiving corn plant.

As used herein, the phrase “at least one” in reference to something(e.g., any object, method step, etc.) means one or more of thatsomething. For example, “at least one plant” or “at least one plants”each means one or more plants. Accordingly, “at least one” can includeone or a plurality. Thus, where the present disclosure provides “one ormore” of something or “at least one” of something, then the descriptionfurther supports a plurality of that something.

In an aspect, any mutant allele, mutation (e.g., without being limiting,a deletion, inversion, insertion, or combinations thereof) or transgeneprovided herein is present in the genome of a female plant. In anaspect, any mutant allele, mutation (e.g., without being limiting, adeletion, inversion, insertion, or combinations thereof) or transgeneprovided herein is present in the genome of a male plant.

In an aspect, this disclosure provides corn plants comprising a dominantmutant allele. In an aspect, this disclosure provides corn plantscomprising a semi-dominant mutant allele. Dominant alleles are allelesthat mask the contribution of a second allele at the same locus. Adominant allele can be a “dominant negative allele” or a “dominantpositive allele.” Dominant negative alleles, or antimorphs, are allelesthat act in opposition to normal allelic function. A dominant negativeallele typically does not function normally and either directly inhibitsthe activity of a wild-type protein (e.g., through dimerization) orinhibits the activity of a second protein that is required for thenormal function of the wild-type protein (e.g., an activator or adownstream component of a pathway). For example, a dominant negativeallele abrogates or reduces the normal function of an allele in aheterozygous or homozygous state. Dominant positive alleles can increasenormal gene function (e.g., a hypermorph) or provide new functions for agene (e.g., a neomorph). A semi-dominant allele occurs when penetranceof a linked phenotype in individuals heterozygous for the allele is lessthan that which is observed in individuals homozygous for the allele.

Creation of dominant alleles that work in a heterozygous state, canspeed up effective trait development, deployment, and launch of geneediting-derived products in hybrid crops such as corn. Dominant negativealleles have the potential advantage of providing a positive orbeneficial plant trait in a heterozygous state—e.g., when present in asingle copy. As a result, a dominant negative mutant allele can beintroduced through crossing into a progeny plant from a single parentwithout having to introduce the allele from both parent plants as with arecessive allele.

In an aspect, a dominant mutant allele is a dominant negative allele. Inan aspect, a dominant mutant allele is a dominant positive allele.

In an aspect, a dominant mutant allele or a semi-dominant mutant allelecomprises an insertion, an inversion, a deletion, or any combinationthereof as compared to a wildtype allele of a gene.

In an aspect, a mutant allele provided herein is a dominant mutantallele. In an aspect, a mutant allele provided herein is a semi-dominantmutant allele. In an aspect, a mutant allele provided herein is adominant negative mutant allele.

In an aspect, a female corn plant is a modified corn plant. In anotheraspect, a female inbred corn plant is a modified corn plant. In anaspect, a modified plant provided herein is homozygous (or biallelic)for a dominant mutant allele(s) or transgene. In an aspect, a modifiedplant provided herein is homozygous (or biallelic) for a semi-dominantmutant allele(s). In an aspect, a modified plant provided herein ishomozygous (or biallelic) for a dominant negative mutant allele. In anaspect, a modified plant provided herein is heterozygous or hemizygousfor a dominant mutant allele or transgene. In an aspect, a modifiedplant provided herein is heterozygous or hemizygous for a semi-dominantmutant allele or transgene. In an aspect, a modified plant providedherein is heterozygous for a dominant negative mutant allele. As usedherein, “modified”, in the context of plants, seeds, plant components,plant cells, and plant genomes, refers to a state containing changes orvariations from their natural or native state. According to an aspect, amodified corn plant, which may be a female corn plant, has a shorterplant height as compared to a control plant and/or a male corn plant.

As used herein, the term “control plant” (or likewise a “control” plantseed, plant part, plant cell and/or plant genome) refers to a plant (orplant seed, plant part, plant cell and/or plant genome) that is used forcomparison to a modified plant (or modified plant seed, plant part,plant cell and/or plant genome) and has the same or similar geneticbackground (e.g., same parental lines, hybrid cross, inbred line,testers, etc.) as the modified plant (or plant seed, plant part, plantcell and/or plant genome), except for a transgenic event and/or genomeedit(s) (e.g., an inversion or antisense insertion) affecting one ormore genes. For example, a control plant may be an inbred line that isthe same as the inbred line used to make the modified plant, or acontrol plant may be the product of the same hybrid cross of inbredparental lines as the modified plant, except for the absence in thecontrol plant of any transgenic or genome edit(s) affecting one or moreGA oxidase or br2 genes. Similarly, an unmodified control plant refersto a plant that shares a substantially similar or essentially identicalgenetic background as a modified plant, but without the one or moreengineered changes to the genome (e.g., transgene, mutation or edit) ofthe modified plant. For purposes of comparison to a modified plant,plant seed, plant part, plant cell and/or plant genome, a “wild-typeplant” (or likewise a “wild-type” plant seed, plant part, plant celland/or plant genome) refers to a non-transgenic and non-genome editedcontrol plant, plant seed, plant part, plant cell and/or plant genome.As used herein, a “control” plant, plant seed, plant part, plant celland/or plant genome may also be a plant, plant seed, plant part, plantcell and/or plant genome having a similar (but not the same oridentical) genetic background to a modified plant, plant seed, plantpart, plant cell and/or plant genome, if deemed sufficiently similar forcomparison of the characteristics or traits to be analyzed.

In an aspect, this disclosure provides a dominant mutant allele in aGA20 oxidase_3 gene. In an aspect, this disclosure provides a dominantmutant allele in a GA20 oxidase_5 gene. In an aspect, this disclosureprovides a dominant mutant allele in a GA3 oxidase gene. In an aspect,this disclosure provides a dominant mutant allele in a brachytic2 gene.

In an aspect, this disclosure provides a semi-dominant mutant allele ina GA20 oxidase_3 gene. In an aspect, this disclosure provides asemi-dominant mutant allele in a GA20 oxidase_5 gene. In an aspect, thisdisclosure provides a semi-dominant mutant allele in a GA3 oxidase gene.In an aspect, this disclosure provides a semi-dominant mutant allele ina brachytic2 gene.

In an aspect, this disclosure provides a dominant negative mutant allelein a GA20 oxidase_3 gene. In an aspect, this disclosure provides adominant negative mutant allele in a GA20 oxidase_5 gene. In an aspect,this disclosure provides a dominant negative mutant allele in a GA3oxidase gene. In an aspect, this disclosure provides a dominant negativemutant allele in a brachytic2 gene.

In an aspect, a female corn plant is homozygous (or biallelic) for amutant allele or transgene provided herein. In an aspect, a female cornplant is heterozygous for a mutant allele provided herein. In an aspect,a male corn plant is homozygous (or biallelic) for a mutant allele ortransgene provided herein. In an aspect, a male corn plant isheterozygous for a mutant allele provided herein. In an aspect, a cornplant is homozygous (or biallelic) for a mutant allele or transgeneprovided herein. In an aspect, a corn plant is heterozygous for a mutantallele provided herein.

In an aspect, a dominant negative mutant allele generates an antisenseRNA transcript capable of triggering suppression of an unmodified orwildtype allele of the gene. In an aspect, a dominant negative mutantallele encodes a truncated protein as compared to an unmodified alleleof the gene. In an aspect, a dominant negative mutant allele generatesat least one RNA transcript capable of forming a hairpin-loop secondarystructure. In an aspect, the coding sequence of a dominant negativemutant allele is operably linked to a promoter of the native copy of thegene. In an aspect, a dominant negative mutant allele comprises aheterologous non-coding RNA target site in the endogenous locus of thegene. In an aspect, a dominant negative mutant allele comprises aninverted copy of the gene, or a portion thereof, adjacent to a wildtypecopy of the gene at the endogenous locus of the gene. As used herein, a“portion” of a gene refers to at least 10, at least 20, at least 30, atleast 40, at least 50, at least 100, at least 250, at least 500, atleast 1000, or at least 2500 consecutive nucleotides of the gene. Asused herein, “adjacent” refers to a nucleic acid sequence that is inclose proximity, or next to another nucleic acid sequence. In oneaspect, adjacent nucleic acid sequences are physically linked. Inanother aspect, adjacent nucleic acid sequences or genes are immediatelynext to each other such that there are no intervening nucleotidesbetween the end of a first nucleic acid sequence and the start of asecond nucleic acid sequence. In an aspect, a first gene and a secondgene are adjacent to each other if they are separated by less than50,000, less than 25,000, less than 10,000, less than 9000, less than8000, less than 7000, less than 6000, less than 5000, less than 4000,less than 3000, less than 2500, less than 2000, less than 1750, lessthan 1500, less than 1250, less than 1000, less than 900, less than 800,less than 700, less than 600, less than 500, less than 400, less than300, less than 200, less than 100, less than 75, less than 50, less than25, less than 20, less than 10, less than 5, less than 4, less than 3,less than 2, or less than 1 nucleotides.

In an aspect, a dominant negative mutant allele comprises a deletion ofa portion of a chromosome between a first region of the gene and asecond region of the gene, wherein an antisense RNA transcript of thefirst region of the gene is generated following the deletion of theportion of the chromosome. In an aspect, a dominant negative mutantallele comprises a first promoter and a second promoter separated by anintervening region, wherein the first promoter and the second promoterare positioned in opposite orientations, wherein the second promotergenerates at least one antisense RNA transcript, and wherein expressionof the gene is reduced as compared to a control corn plant that lacksthe dominant negative mutant allele. In an aspect, a dominant negativemutant allele comprises a tissue-specific or tissue-preferred promoterinserted into the gene in reverse orientation as compared to the nativepromoter of the gene, wherein the tissue-specific or tissue-preferredpromoter generates at least one antisense RNA transcript, and whereinexpression of the gene is reduced as compared to a control corn plantthat lacks the dominant negative mutant allele.

In an aspect, this disclosure provides corn plants comprising a dominantor semi-dominant transgene or mutant allele of a gene that causes ashort stature phenotype. As used herein, a “short stature phenotype”refers to a dwarf corn plant, a semi-dwarf corn plant, or a brachyticcorn plant. In an aspect, a plant is homozygous (or biallelic) for atransgene. In an aspect, a plant is heterozygous for a transgene. In anaspect, a plant is hemizygous for a transgene. In an aspect, a cornplant is homozygous (or biallelic) for a transgene provided herein. Inan aspect, a corn plant is heterozygous for a transgene provided herein.In an aspect, a corn plant is hemizygous for a transgene providedherein.

In an aspect, a transgene comprises a recombinant polynucleotideencoding an RNA molecule that suppresses expression of an endogenousGA20 oxidase gene. In an aspect, a transgene comprises a recombinantpolynucleotide encoding an RNA molecule that suppresses expression of anendogenous GA20 oxidase_3 gene. In an aspect, a transgene comprises arecombinant polynucleotide encoding an RNA molecule that suppressesexpression of an endogenous GA20 oxidase_5 gene. In an aspect, atransgene comprises a recombinant polynucleotide encoding an RNAmolecule that suppresses expression of an endogenous GA3 oxidase gene.In an aspect, a transgene comprises a recombinant polynucleotideencoding an RNA molecule that suppresses expression of an endogenous br2gene. In an aspect, a recombinant polynucleotide is operably linked to apromoter. In an aspect, a transgene comprises a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein.

As used herein, the term “plurality” in reference to an item means twoor more of such items. For example, a “plurality of plants” means two ormore plants.

In an aspect, corn plants disclosed herein are selected from thesubspecies Zea mays L. ssp. mays. In an additional aspect, corn plantsdisclosed herein are selected from the group Zea mays L. subsp. maysIndentata, otherwise known as dent corn. In another aspect, corn plantsdisclosed herein are selected from the group Zea mays L. subsp. maysIndurata, otherwise known as flint corn. In an aspect, corn plantsdisclosed herein are selected from the group Zea mays L. subsp. maysSaccharata, otherwise known as sweet corn. In another aspect, cornplants disclosed herein are selected from the group Zea mays L. subsp.mays Amylacea, otherwise known as flour corn. In a further aspect, cornplants disclosed herein are selected from the group Zea mays L. subsp.mays Everta, otherwise known as popcorn. Plants disclosed herein alsoinclude hybrids, inbreds, partial inbreds, or members of defined orundefined populations.

Growers must balance crop prices, standability, plant health, and kernelmoisture content when determining when to harvest a corn field. Asprovided herein, corn plants with better standability, such as dwarfcorn plants, semi-dwarf corn plants, and brachytic corn plants, areresistant to lodging and thus can remain in the field for a longerperiod of time prior to harvest without significant loss of yield, orwith improved yield relative to taller corn plants (especially whencompared to corn plants that have lodged). According to aspects of thepresent disclosure, the improved standability of short stature cornplants provides growers and seed producers with more flexibility on whento harvest, allows more time for drying down seed or grain prior toharvest, and/or enables or improves direct harvest applications,particularly in corn seed production operations. As used herein, “directharvesting” refers to the harvesting of crop seeds from plants with acombine harvester in the field with little or no further drying or otherprocessing or desiccation steps prior to seed storage. As used herein,“standability” refers to the ability of a plant or a plurality,population or field of plants, such as a corn plant or a plurality,population or field of corn plants, to stand upright in a position thatenables the plant(s) to be harvested by standard farm equipment (e.g., acombine harvester). As used herein, “lodging” can refer to either “stalklodging” or “root lodging.” Stalk lodging occurs when the corn plantstalk is severely bent or broken below the ear. Root lodging occurs whenthe corn plant is leaning at an angle (e.g., greater than or equal to45° relative to perpendicular from the ground, or at an angle less than45° relative to the ground). Lodged corn plants, whether stalk lodgedand/or root lodged, severely limit harvestability by standard farmequipment (e.g., a combine harvester) resulting in up to 100% yield lossof the lodged corn plants.

In an aspect, a modified corn plant provided herein has improved lodgingresistance relative to an unmodified control plant.

Growers will leave harvestable corn standing in a field to reduce thekernel moisture content of the grain. Optimal kernel moisture contentcan vary by growing region and by individual grower. Typically, kernelmoisture content decreases the longer the corn plants are left in thefield (e.g., the longer the period of time between fertilization orreaching maturity and harvest). However, extending the period of timebetween fertilization (or reaching maturity or some other developmentalstage) and harvesting can increase the chance that plants will lodge,which can result in significant decreases in yield (even up to 100%). Asprovided herein, by providing plants with reduced heights that have highstandability performance (i.e., resistance to lodging), growers areenabled to allow for greater periods of time until harvest withoutincreasing (or significantly or substantially increasing) their risk ofyield loss due to lodging. Typical grain moisture contents forharvesting corn are between 15% and 25%, although wider ranges of 13-30%or higher are possible. According to present embodiments, corn plantsmay be left in the field for a longer period of time after reaching agiven grain moisture content percentage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field at least 30 days, at least 40 days, at least50 days, at least 60 days, at least 70 days, at least 80 days, at least90 days, at least 100 days, or at least 110 days after at least 50%, atleast 60%, at least 70%, at least 80%, at least 90% or 100% of said cornplants have reached R3 stage, wherein fewer than or equal to 50% of saidcorn plants have lodged at the time of harvest. In another aspect, amethod comprises harvesting a plurality of corn plants from a field atleast 30 days, at least 40 days, at least 50 days, at least 60 days, atleast 70 days, at least 80 days, at least 90 days, at least 100 days, orat least 110 days after at least 50%, at least 60%, at least 70%, atleast 80%, at least 90% or 100% of said corn plants have reached R3stage, wherein average kernel moisture content is less than or equal to30% or the kernel moisture content of a corn plant of the plurality ofcorn plants is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest. Inanother aspect, a method comprising harvesting a plurality of cornplants from a field at least 30 days, at least 40 days, at least 50days, at least 60 days, at least 70 days, at least 80 days, at least 90days, at least 100 days, or at least 110 days after at least 50%, atleast 60%, at least 70%, at least 80%, at least 90% or 100% of said cornplants have reached R3 stage, wherein the average yield of said field isat least 170 bushels per acre, and wherein fewer than or equal to 50% ofsaid corn plants have lodged at the time of harvest.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field at least 30 days, at least 40 days, at least50 days, at least 60 days, at least 70 days, at least 80 days at least90 days, at least 100 days, or at least 110 days after fertilization orsilking of said plurality of corn plants, wherein fewer than or equal to50% of said corn plants have lodged at the time of harvest. In anotheraspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field at least 30 days, at least 40 days, at least 50days, at least 60 days, at least 70 days, at least 80 days at least 90days, at least 100 days, or at least 110 days after fertilization orsilking of said plurality of corn plants, wherein the average kernelmoisture content is less than or equal to 30% or the kernel moisturecontent of a corn plant of the plurality of corn plants is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest. In another aspect, a method providedherein comprises harvesting a plurality of corn plants from a field atleast 30 days, at least 40 days, at least 50 days, at least 60 days, atleast 70 days, at least 80 days at least 90 days, at least 100 days, orat least 110 days after at fertilization or silking of said plurality ofcorn plants, wherein the average yield of said field is at least 170bushels per acre, and wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field when, or at least 1 day after, the averagekernel moisture content of said plurality of corn plants is between 10%and 30%, or less than or equal to 30%, or the kernel moisture content ofa corn plant of the plurality of corn plants is between 10% and 30%, orless than or equal to 30%, wherein fewer than or equal to 50% of saidcorn plants have lodged at the time of harvest. In an aspect, methodsprovided herein comprise harvesting a plurality of corn plants from afield when, or at least 1 day after, the average kernel moisture contentof said plurality of corn plants is between 15% and 25%, or less than orequal to 25%, or less than or equal to 20%, or less than or equal to15%, or the kernel moisture content of a corn plant of the plurality ofcorn plants is between 15% and 25%, or less than or equal to 25%, orless than or equal to 20%, or less than or equal to 15%, wherein fewerthan or equal to 50% of said corn plants have lodged at the time ofharvest. In each of these aspects, the average yield of said plants in afield may be at least 170 bushels per acre.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field at least 30 days, at least 40 days, at least50 days, at least 60 days, at least 70 days, at least 80 days, at least90 days, at least 100 days, or at least 110 days after fertilization orsilking of at least 50%, at least 60%, at least 70%, at least 80%, atleast 85%, at least 90%, or 100% of said plurality of corn plants,wherein fewer than or equal to 50% of said corn plants have lodged atthe time of harvest. In another aspect, a method provided hereincomprises harvesting a plurality of corn plants from a field at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, at least 100 days, or at least110 days after fertilization or silking of at least 50%, at least 60%,at least 70%, at least 80%, at least 85%, at least 90%, or 100% of saidplurality of corn plants, wherein the average kernel moisture content isless than or equal to 30% or the kernel moisture content of a corn plantof the plurality of corn plants is less than or equal to 30%, andwherein fewer than or equal to 50% of said corn plants have lodged atthe time of harvest. In another aspect, a method provided hereincomprises harvesting a plurality of corn plants from a field at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, at least 100 days, or at least110 days after at fertilization or silking of at least 50%, at least60%, at least 70%, at least 80%, at least 85%, at least 90%, or 100% ofsaid plurality of corn plants, wherein the average yield of said fieldis at least 170 bushels per acre, and wherein fewer than or equal to 50%of said corn plants have lodged at the time of harvest.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field when, or at least 1 day after, the averagekernel moisture content of at least 50%, at least 60%, at least 70%, atleast 80%, at least 85%, at least 90%, or 100% of said plurality of cornplants is between 10% and 30%, or less than or equal to 30%, or thekernel moisture content of a corn plant of the plurality of corn plantsis between 10% and 30%, or less than or equal to 30%, wherein fewer thanor equal to 50% of said corn plants have lodged at the time of harvest.In an aspect, methods provided herein comprise harvesting a plurality ofcorn plants from a field when, or at least 1 day after, the averagekernel moisture content of at least 50%, at least 60%, at least 70%, atleast 80%, at least 85%, at least 90%, or 100% of said plurality of cornplants is between 15% and 25%, or less than or equal to 25%, or lessthan or equal to 20%, or less than or equal to 15%, or the kernelmoisture content of a corn plant of the plurality of corn plants isbetween 15% and 25%, or less than or equal to 25%, or less than or equalto 20%, or less than or equal to 15%, wherein fewer than or equal to 50%of said corn plants have lodged at the time of harvest. In each of theseaspects, the average yield of said plants in a field may be at least 170bushels per acre.

In an aspect, a method provided herein further comprises growing aplurality of corn plants in a corn field prior to harvesting theplurality of corn plants.

In an aspect, corn plants provided herein are inbred corn plants. Asused herein, the term “inbred” means a line that has been bred forgenetic homogeneity. In another aspect, corn plants provided herein arehybrid corn plants. As used herein, the term “hybrid” means a progeny ofmating between at least two genetically dissimilar parents or inbreds.In an aspect, corn plants provided herein are transgenic, mutant and/oredited corn plants.

In an aspect, at least 10% of the corn plants in a field are inbred cornplants. In an aspect, at least 20% of the corn plants in a field areinbred corn plants. In an aspect, at least 30% of the corn plants in afield are inbred corn plants. In an aspect, at least 40% of the cornplants in a field are inbred corn plants. In an aspect, at least 50% ofthe corn plants in a field are inbred corn plants. In an aspect, atleast 60% of the corn plants in a field are inbred corn plants. In anaspect, at least 70% of the corn plants in a field are inbred cornplants. In an aspect, at least 80% of the corn plants in a field areinbred corn plants. In an aspect, at least 90% of the corn plants in afield are inbred corn plants. In an aspect, 100% of the corn plants in afield are inbred corn plants.

In an aspect, between 1% and 100% of the corn plants in a field areinbred corn plants. In an aspect, between 10% and 100% of the cornplants in a field are inbred corn plants. In an aspect, between 20% and100% of the corn plants in a field are inbred corn plants. In an aspect,between 30% and 100% of the corn plants in a field are inbred cornplants. In an aspect, between 40% and 100% of the corn plants in a fieldare inbred corn plants. In an aspect, between 50% and 100% of the cornplants in a field are inbred corn plants. In an aspect, between 60% and100% of the corn plants in a field are inbred corn plants. In an aspect,between 70% and 100% of the corn plants in a field are inbred cornplants. In an aspect, between 80% and 100% of the corn plants in a fieldare inbred corn plants. In an aspect, between 90% and 100% of the cornplants in a field are inbred corn plants.

In an aspect, at least 10% of the corn plants in a field are hybrid cornplants. In an aspect, at least 20% of the corn plants in a field arehybrid corn plants. In an aspect, at least 30% of the corn plants in afield are hybrid corn plants. In an aspect, at least 40% of the cornplants in a field are hybrid corn plants. In an aspect, at least 50% ofthe corn plants in a field are hybrid corn plants. In an aspect, atleast 60% of the corn plants in a field are hybrid corn plants. In anaspect, at least 70% of the corn plants in a field are hybrid cornplants. In an aspect, at least 80% of the corn plants in a field arehybrid corn plants. In an aspect, at least 90% of the corn plants in afield are hybrid corn plants. In an aspect, 100% of the corn plants in afield are hybrid corn plants.

In an aspect, between 1% and 100% of the corn plants in a field arehybrid corn plants. In an aspect, between 10% and 100% of the cornplants in a field are hybrid corn plants. In an aspect, between 20% and100% of the corn plants in a field are hybrid corn plants. In an aspect,between 30% and 100% of the corn plants in a field are hybrid cornplants. In an aspect, between 40% and 100% of the corn plants in a fieldare hybrid corn plants. In an aspect, between 50% and 100% of the cornplants in a field are hybrid corn plants. In an aspect, between 60% and100% of the corn plants in a field are hybrid corn plants. In an aspect,between 70% and 100% of the corn plants in a field are hybrid cornplants. In an aspect, between 80% and 100% of the corn plants in a fieldare hybrid corn plants. In an aspect, between 90% and 100% of the cornplants in a field are hybrid corn plants.

In another aspect, a corn plant provided herein is a semi-dwarf cornplant. As used herein, a “semi-dwarf plant” refers to a plant having astature or height that is reduced relative to a control wild-type plantby at least 5%, at least 10%, at least 15%, at least 20%, at least 25%,at least 30%, at least 35%, at least 40%, at least 45%, or at least 50%.Such a semi-dwarf plant can be characterized by a reduced stem, stalk,or trunk length when compared to the control wild-type plant undercomparable growth conditions, which can result from fewer internodes orshorter average internode length. As used herein, an “internode” refersto the region between two nodes on a corn stalk, and a “node” refers tothe point on the corn stalk (e.g., stem) where leaves and/or earsoriginate.

In an aspect, at least 10% of the corn plants in a field are semi-dwarfcorn plants. In an aspect, at least 20% of the corn plants in a fieldare semi-dwarf corn plants. In an aspect, at least 30% of the cornplants in a field are semi-dwarf corn plants. In an aspect, at least 40%of the corn plants in a field are semi-dwarf corn plants. In an aspect,at least 50% of the corn plants in a field are semi-dwarf corn plants.In an aspect, at least 60% of the corn plants in a field are semi-dwarfcorn plants. In an aspect, at least 70% of the corn plants in a fieldare semi-dwarf corn plants. In an aspect, at least 80% of the cornplants in a field are semi-dwarf corn plants. In an aspect, at least 90%of the corn plants in a field are semi-dwarf corn plants. In an aspect,100% of the corn plants in a field are semi-dwarf corn plants.

In an aspect, between 1% and 100% of the corn plants in a field aresemi-dwarf corn plants. In an aspect, between 10% and 100% of the cornplants in a field are semi-dwarf corn plants. In an aspect, between 20%and 100% of the corn plants in a field are semi-dwarf corn plants. In anaspect, between 30% and 100% of the corn plants in a field aresemi-dwarf corn plants. In an aspect, between 40% and 100% of the cornplants in a field are semi-dwarf corn plants. In an aspect, between 50%and 100% of the corn plants in a field are semi-dwarf corn plants. In anaspect, between 60% and 100% of the corn plants in a field aresemi-dwarf corn plants. In an aspect, between 70% and 100% of the cornplants in a field are semi-dwarf corn plants. In an aspect, between 80%and 100% of the corn plants in a field are semi-dwarf corn plants. In anaspect, between 90% and 100% of the corn plants in a field aresemi-dwarf corn plants.

In an aspect, a corn plant provided herein is a dwarf corn plant. Asused herein, a “dwarf” plant refers to an atypically small plant.Generally, such a “dwarf plant” has a stature or height that is reducedfrom that of a control wild-type plant (e.g., a sibling plant comprisingall other traits except the dwarf trait) by at least 30%, at least 35%,at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, orat least 75%.

In an aspect, at least 10% of the corn plants in a field are dwarf cornplants. In an aspect, at least 20% of the corn plants in a field aredwarf corn plants. In an aspect, at least 30% of the corn plants in afield are dwarf corn plants. In an aspect, at least 40% of the cornplants in a field are dwarf corn plants. In an aspect, at least 50% ofthe corn plants in a field are dwarf corn plants. In an aspect, at least60% of the corn plants in a field are dwarf corn plants. In an aspect,at least 70% of the corn plants in a field are dwarf corn plants. In anaspect, at least 80% of the corn plants in a field are dwarf cornplants. In an aspect, at least 90% of the corn plants in a field aredwarf corn plants. In an aspect, 100% of the corn plants in a field aredwarf corn plants.

In an aspect, between 1% and 100% of the corn plants in a field aredwarf corn plants. In an aspect, between 10% and 100% of the corn plantsin a field are dwarf corn plants. In an aspect, between 20% and 100% ofthe corn plants in a field are dwarf corn plants. In an aspect, between30% and 100% of the corn plants in a field are dwarf corn plants. In anaspect, between 40% and 100% of the corn plants in a field are dwarfcorn plants. In an aspect, between 50% and 100% of the corn plants in afield are dwarf corn plants. In an aspect, between 60% and 100% of thecorn plants in a field are dwarf corn plants. In an aspect, between 70%and 100% of the corn plants in a field are dwarf corn plants. In anaspect, between 80% and 100% of the corn plants in a field are dwarfcorn plants. In an aspect, between 90% and 100% of the corn plants in afield are dwarf corn plants.

As used herein, the term “polynucleotide” refers to a nucleic acidmolecule containing multiple nucleotides and generally comprises atleast 2, at least 5, at least 10, at least 20, at least 30, at least 40,at least 50, at least 100, at least 250, at least 500, at least 1,000,at least 1,500, at least 2,000, at least 2,500, at least 3,000, at least5,000, or at least 10,000 nucleotide bases. As an example, apolynucleotide provided herein can be a plasmid. The use of the terms“polynucleotide” or “nucleic acid molecule” is not intended to limit thepresent disclosure to polynucleotides comprising deoxyribonucleic acid(DNA). For example, ribonucleic acid (RNA) molecules are alsoenvisioned. Those of ordinary skill in the art will recognize thatpolynucleotides and nucleic acid molecules can comprise ribonucleotidesand combinations of ribonucleotides and deoxyribonucleotides. Suchdeoxyribonucleotides and ribonucleotides include both naturallyoccurring molecules and synthetic analogues. The polynucleotides of thepresent disclosure also encompass all forms of sequences including, butnot limited to, single-stranded forms, double-stranded forms, hairpins,stem-and-loop structures, and the like. In an aspect, a nucleic acidmolecule provided herein is a DNA molecule. In another aspect, a nucleicacid molecule provided herein is an RNA molecule. In an aspect, anucleic acid molecule provided herein is single-stranded. In anotheraspect, a nucleic acid molecule provided herein is double-stranded. Inan aspect, a polynucleotide provided herein is single-stranded. Inanother aspect, a polynucleotide provided herein is double-stranded.

A non-coding RNA molecule can act as a suppression element that targetsone or more gene(s) in a plant cell, such as one or more endogenous br2,GA20 or GA3 oxidase gene(s), or as a RNA molecule, such as a guide RNA,etc., that guides a sequence-specific nuclease to cut and trigger agenome editing event at a target site in the genome. Non-limitingexamples of non-coding RNA molecules include a microRNA (miRNA), a miRNAprecursor (pre-miRNA), a small interfering RNA (siRNA), a small RNA(18-26 nt in length) and precursor encoding same, a heterochromaticsiRNA (hc-siRNA), a Piwi-interacting RNA (piRNA), a hairpin doublestrand RNA (hairpin dsRNA), a trans-acting siRNA (ta-siRNA), a naturallyoccurring antisense siRNA (nat-siRNA), a CRISPR RNA (crRNA), a tracerRNA (tracrRNA), a guide RNA (gRNA), and a single-guide RNA (sgRNA). Inan aspect, a non-coding RNA provided herein is selected from the groupconsisting of a microRNA, a small interfering RNA, a secondary smallinterfering RNA, a transfer RNA, a ribosomal RNA, a trans-acting smallinterfering RNA, a naturally occurring antisense small interfering RNA,a heterochromatic small interfering RNA, and precursors thereof. Inanother aspect, a non-coding RNA provided herein is selected from thegroup consisting of a miRNA, a pre-miRNA, a siRNA, a hc-siRNA, a piRNA,a hairpin dsRNA, a ta-siRNA, a nat-siRNA, a crRNA, a tracrRNA, a gRNA,and a sgRNA. In another aspect, a non-coding RNA provided herein is amiRNA. In another aspect, a non-coding RNA provided herein is a siRNA.

As used herein, a “stem-loop structure” refers to a secondary structurein a RNA molecule having a double stranded region (e.g., stem) made upby two annealing RNA strands, sequences or segments of the RNA molecule,connected by a single stranded intervening RNA sequence of the RNAmolecule (e.g., a loop or hairpin). A “stem-loop structure” of a RNAmolecule can have a more complicated secondary RNA structure, forexample, comprising self-annealing double stranded RNA sequences havinginternal mismatches, bulges and/or loops.

As used herein, a “native sequence” refers to a nucleic acid sequencenaturally present in its original chromosomal location.

As used herein, a “wild-type gene” or “wild-type allele” refers to agene or allele having a sequence or genotype that is most common in aparticular plant species or another sequence or genotype having onlynatural variations, polymorphisms, or other silent mutations relative tothe most common sequence or genotype that do not significantly impactthe expression and activity of the gene or allele. Indeed, a “wild-type”gene or allele contains no variation, polymorphism, or any other type ofmutation that substantially affects the normal function, activity,expression, or phenotypic consequence of the gene or allele relative tothe most common sequence or genotype.

The terms “percent identity” or “percent identical” as used herein inreference to two or more nucleotide or protein sequences is calculatedby (i) comparing two optimally aligned sequences (nucleotide or protein)over a window of comparison, (ii) determining the number of positions atwhich the identical nucleic acid base (for nucleotide sequences) oramino acid residue (for proteins) occurs in both sequences to yield thenumber of matched positions, (iii) dividing the number of matchedpositions by the total number of positions in the window of comparison,and then (iv) multiplying this quotient by 100% to yield the percentidentity. For purposes of calculating “percent identity” between DNA andRNA sequences, a uracil (U) of a RNA sequence is considered identical toa thymine (T) of a DNA sequence. If the window of comparison is definedas a region of alignment between two or more sequences (i.e., excludingnucleotides at the 5′ and 3′ ends of aligned polynucleotide sequences,or amino acids at the N-terminus and C-terminus of aligned proteinsequences, that are not identical between the compared sequences), thenthe “percent identity” may also be referred to as a “percent alignmentidentity”. If the “percent identity” is being calculated in relation toa reference sequence without a particular comparison window beingspecified, then the percent identity is determined by dividing thenumber of matched positions over the region of alignment by the totallength of the reference sequence. Accordingly, for purposes of thepresent disclosure, when two sequences (query and subject) are optimallyaligned (with allowance for gaps in their alignment), the “percentidentity” for the query sequence is equal to the number of identicalpositions between the two sequences divided by the total number ofpositions in the query sequence over its length (or a comparisonwindow), which is then multiplied by 100%.

For optimal alignment of sequences to calculate their percent identity,various pair-wise or multiple sequence alignment algorithms and programsare known in the art, such as ClustalW, or Basic Local Alignment SearchTool® (BLAST®), etc., that may be used to compare the sequence identityor similarity between two or more nucleotide or protein sequences.Although other alignment and comparison methods are known in the art,the alignment between two sequences (including the percent identityranges described above) may be as determined by the ClustalW or BLAST®algorithm, see, e.g., Chenna R. et al., “Multiple sequence alignmentwith the Clustal series of programs,” Nucleic Acids Research 31:3497-3500 (2003); Thompson J D et al., “Clustal W: Improving thesensitivity of progressive multiple sequence alignment through sequenceweighting, position-specific gap penalties and weight matrix choice,”Nucleic Acids Research 22: 4673-4680 (1994); and Larkin M A et al.,“Clustal W and Clustal X version 2.0,” Bioinformatics 23: 2947-48(2007); and Altschul, S. F., Gish, W., Miller, W., Myers, E. W. &Lipman, D. J. (1990) “Basic local alignment search tool.” J. Mol. Biol.215:403-410 (1990), the entire contents and disclosures of which areincorporated herein by reference.

The terms “percent complementarity” or “percent complementary”, as usedherein in reference to two nucleotide sequences, is similar to theconcept of percent identity but refers to the percentage of nucleotidesof a query sequence that optimally base-pair or hybridize to nucleotidesof a subject sequence when the query and subject sequences are linearlyarranged and optimally base paired without secondary folding structures,such as loops, stems or hairpins. Such a percent complementarity may bebetween two DNA strands, two RNA strands, or a DNA strand and a RNAstrand. The “percent complementarity” is calculated by (i) optimallybase-pairing or hybridizing the two nucleotide sequences in a linear andfully extended arrangement (i.e., without folding or secondarystructures) over a window of comparison, (ii) determining the number ofpositions that base-pair between the two sequences over the window ofcomparison to yield the number of complementary positions, (iii)dividing the number of complementary positions by the total number ofpositions in the window of comparison, and (iv) multiplying thisquotient by 100% to yield the percent complementarity of the twosequences. Optimal base pairing of two sequences may be determined basedon the known pairings of nucleotide bases, such as G-C, A-T, and A-U,through hydrogen bonding. If the “percent complementarity” is beingcalculated in relation to a reference sequence without specifying aparticular comparison window, then the percent identity is determined bydividing the number of complementary positions between the two linearsequences by the total length of the reference sequence. Thus, forpurposes of the present disclosure, when two sequences (query andsubject) are optimally base-paired (with allowance for mismatches ornon-base-paired nucleotides but without folding or secondarystructures), the “percent complementarity” for the query sequence isequal to the number of base-paired positions between the two sequencesdivided by the total number of positions in the query sequence over itslength (or by the number of positions in the query sequence over acomparison window), which is then multiplied by 100%.

As used herein, with respective to a given sequence, a “complement”, a“complementary sequence” and a “reverse complement” are usedinterchangeably. All three terms refer to the inversely complementarysequence of a nucleotide sequence, i.e. to a sequence complementary to agiven sequence in reverse order of the nucleotides. As an example, thereverse complement of a nucleotide sequence having the sequence5′-atggttc-3′ is 5′-gaaccat-3′.

As used herein, the term “antisense” refers to DNA or RNA sequences thatare complementary to a specific DNA or RNA sequence. Antisense RNAmolecules are single-stranded nucleic acids which can combine with asense RNA strand or sequence or mRNA to form duplexes due tocomplementarity of the sequences. The term “antisense strand” refers toa nucleic acid strand that is complementary to the “sense” strand. The“sense strand” of a gene or locus is the strand of DNA or RNA that hasthe same sequence as a RNA molecule transcribed from the gene or locus(with the exception of Uracil in RNA and Thymine in DNA).

As used herein, an “inverted genomic fragment” refers to a genomicsegment that is inverted in the genome such that the original sensestrand and anti sense strand sequences are reversed or switched in theopposite orientation for the entire genomic segment.

As used herein, in the context of a “corresponding endogenous sequence”or a “corresponding endogenous DNA segment,” an endogenous sequence orendogenous DNA segment is considered to correspond to another sequenceor DNA segment (e.g., an non-endogenous, introduced or inserted sequenceor DNA segment) when the sequences or DNA segments share sufficientsequence homology, identity, or complementarity.

The term “operably linked” refers to a functional linkage between apromoter or other regulatory element and an associated transcribable DNAsequence or coding sequence of a gene (or transgene), such that thepromoter, etc., operates or functions to initiate, assist, affect,cause, and/or promote the transcription and expression of the associatedtranscribable DNA sequence or coding sequence, at least in certaincell(s), tissue(s), developmental stage(s), and/or condition(s). Twotranscribable DNA sequences can also be “operably linked” to each otherif their transcription is subject to the control of a common promoter orother regulatory element. In an aspect, a promoter is selected from thegroup consisting of a constitutive promoter, an inducible promoter, atissue-specific promoter, and a tissue-preferred promoter. In an aspect,a promoter is a native promoter.

As used herein, an “encoding region” or “coding region” refers to aportion of a polynucleotide that encodes a functional unit or molecule(e.g., without being limiting, a mRNA, protein, or non-coding RNAsequence or molecule). An “encoding region” or “coding region” cancontain, for example, one or more exons, one or more introns, a 5′-UTR,a 3′-UTR, or any combination thereof.

As used herein, an “intervening region” or “intervening sequence” refersto a polynucleotide sequence between a physically linked firstpolynucleotide sequence and second polynucleotide sequence. Theintervening sequence may form a loop, and the first and second sequencesmay hybridize to form a stem, of a stem-loop structure. In one aspect,an intervening region or intervening sequence comprises at least 1, atleast 2, at least 3, at least 4, at least 5, at least 10, at least 25,at least 50, at least 100, at least 150, at least 200, at least 250, atleast 500, at least 1000, at least 1250, at least 1500, at least 1750,at least 2000, at least 2500, at least 3000, at least 4000, at least5000, at least 6000, at least 7000, at least 8000, at least 9000, atleast 10,000, at least 15,000, at least 20,000, at least 25,000, or atleast 50,000 nucleotides. In one aspect, an intervening region orintervening sequence comprises a DNA sequence. In one aspect, anintervening region or intervening sequence comprises an RNA sequence. Inone aspect, an intervening region or intervening sequences comprises anendogenous or native nucleic acid sequence. In another aspect, anintervening region or intervening sequences comprises a transgenic orexogenous nucleic acid sequence. In one aspect, an intervening region orintervening sequences comprises an endogenous or native nucleic acidsequence and a transgenic or exogenous nucleic acid sequence.

The term “recombinant” in reference to a polynucleotide (DNA or RNA)molecule, protein, construct, vector, etc., refers to a polynucleotideor protein molecule or sequence that is man-made and not normally foundin nature, and/or is present in a context in which it is not normallyfound in nature, including a polynucleotide (DNA or RNA) molecule,protein, construct, etc., comprising a combination of two or morepolynucleotide or protein sequences that would not naturally occurtogether in the same manner without human intervention, such as apolynucleotide molecule, protein, construct, etc., comprising at leasttwo polynucleotide or protein sequences that are operably linked butheterologous with respect to each other. For example, the term“recombinant” can refer to any combination of two or more DNA or proteinsequences in the same molecule (e.g., a plasmid, construct, vector,chromosome, protein, etc.) where such a combination is man-made and notnormally found in nature. As used in this definition, the phrase “notnormally found in nature” means not found in nature without humanintroduction. A recombinant polynucleotide or protein molecule,construct, etc., can comprise polynucleotide or protein sequence(s) thatis/are (i) separated from other polynucleotide or protein sequence(s)that exist in proximity to each other in nature, and/or (ii) adjacent to(or contiguous with) other polynucleotide or protein sequence(s) thatare not naturally in proximity with each other. Such a recombinantpolynucleotide molecule, protein, construct, etc., can also refer to apolynucleotide or protein molecule or sequence that has been geneticallyengineered and/or constructed outside of a cell. For example, arecombinant DNA molecule can comprise any engineered or man-madeplasmid, vector, etc., and can include a linear or circular DNAmolecule. Such plasmids, vectors, etc., can contain various maintenanceelements including a prokaryotic origin of replication and selectablemarker, as well as one or more transgenes or expression cassettesperhaps in addition to a plant selectable marker gene, etc.

As used herein, the term “transgene” refers to a recombinant DNAmolecule, construct, or sequence comprising a gene and/or transcribableDNA sequence and integrated or inserted into a plant genome.

As used herein, a “transgenic plant” refers to a plant whose genome hasbeen altered by the integration or insertion of a recombinant DNAmolecule, construct, cassette or sequence for expression of a non-codingRNA molecule, mRNA and/or protein in the plant. A transgenic plantincludes an R₀ plant developed or regenerated from an originallytransformed plant cell(s) as well as progeny transgenic plants in latergenerations or crosses from the R₀ transgenic plant that comprise therecombinant DNA molecule, construct, cassette or sequence. A planthaving an integrated or inserted recombinant DNA molecule, construct,cassette or sequence is considered a transgenic plant even if the plantalso has other mutation(s) or edit(s) that would not themselves beconsidered transgenic.

As used herein, the term “heterologous” can refer broadly to acombination of two or more DNA molecules or sequences, such as apromoter and an associated transcribable DNA sequence, coding sequence,or gene, when such a combination is man-made and not normally found innature. The term “heterologous” in reference to a promoter or otherregulatory sequence in relation to an associated polynucleotide sequence(e.g., a transcribable DNA sequence or coding sequence or gene) is apromoter or regulatory sequence that is not operably linked to suchassociated polynucleotide sequence in nature—e.g., the promoter orregulatory sequence has a different origin relative to the associatedpolynucleotide sequence and/or the promoter or regulatory sequence isnot naturally occurring in a plant species to be transformed with thepromoter or regulatory sequence. For example, a transcribable DNAsequence encoding a non-coding RNA molecule that targets one or more GAoxidase gene(s) for suppression can be operably linked to a heterologousplant-expressible promoter.

As used herein, the term “expression” refers to the process forconverting the genetic information of a gene into a functional unit(without being limiting, for example, a mRNA and/or protein or anon-coding RNA molecule).

As used herein, the terms “suppress,” “suppression,” “inhibit,”“inhibition,” “inhibiting,” and “downregulation” refer to a lowering,reduction or elimination of the expression level of a mRNA and/orprotein encoded by a gene in a plant, plant cell, or plant tissue at oneor more stage(s) of plant development, as compared to the expressionlevel of such mRNA and/or protein in a wild-type or control plant, cell,or tissue at the same stage(s) of plant development. In an aspect, apolynucleotide provided herein can suppress the expression of acomplementary target gene. In another aspect, a non-coding RNA moleculecan suppress the expression of a complementary target gene.

As used herein, a “mutation” refers to an insertion, deletion,substitution, duplication, or inversion of one or more nucleotidesand/or encoded amino acids as compared to a reference or wild-typenucleotide and/or amino acid sequence, which can be introduced by anysuitable mutagenesis or gene editing technique.

There are different ways in which a corn plant can be made to have ashorter semi-dwarf plant height. According to many aspects, a corn plantcan be made shorter or semi-dwarf relative to a control plant bylowering the level(s) of active GAs in one or more tissue(s) of theplant, such as by suppressing, mutating or editing a GA oxidase gene inthe corn plant. In an aspect, a corn plant provided herein comprises arecombinant polynucleotide capable of suppressing expression of one ormore GA20 oxidase and/or GA3 oxidase gene(s) and/or mRNA(s) transcribedtherefrom. Alternatively, a corn plant provided herein comprises one ormore mutation(s) or edit(s) in one or more GA20 oxidase and/or GA3oxidase gene(s). According to other aspects, corn plants can have amutation or edit in an auxin, brassinosteroid, jasmonic acid, cell cycleregulation, and/or other pathway gene(s) that are shown to affect plantheight. According to yet further embodiments, corn plants can be madeshorter by application of one or more chemistries shown to affect plantheight. According to another aspect, a corn plant or plurality of cornplants provided herein can comprise a mutation or edit in one or moreloci or genes, or a transgene targeting such one or more loci or genes,that have been associated with a short stature phenotype in corn, suchas one or more of the following: anther ear 1 (An1), brachytic 1 (Br1),brevis plant 1 (Bv1) or brachytic 3 (br3), crinkly 4 (Cr4), compactplant 2 (Ct2), dwarf plant 1 (d1), dwarf plant 8 (d8), dwarf plant 9(d9), nana plant 1 (Na1), nana plant 2 (Na2), non-chromosomal stripe 3(Nsc3), narrow leaf dwarf/(Nld1), reduced plant 1 (Rd1),semi-dwarf/(Sdw1), semi-dwarf 2 (Sdw2), tangled 1 (Tan1), terminal ear 1(Te1), and vanishing tassel 2 (Vt2). As used herein, a “mutation”includes an edit—i.e., a mutation introduced via a genome editingtechnique.

In an aspect, a corn plant(s) is homozygous for one or more mutation(s)and/or edit(s) in one of the foregoing native corn genes. In an aspect,a corn plant is biallelic for a first mutation and/or edit and a secondmutation and/or edit in one of the foregoing native corn genes.

As used herein, a “brachytic plant” refers to a plant having a mutated,edited or suppressed brachytic gene and a short semi-dwarf height andstature relative to a control plant (e.g., a wild-type sibling plantcomprising all other traits except the brachytic trait) due to ashortening of the average internode length. Such a brachytic mutantplant can have a short semi-dwarf height and stature due to a shorteningof the average internode length. As used herein, a “brachytic gene”, “Brgene” or “br gene”, or “Br gene” refers to any brachytic gene in a cornplant that when suppressed, mutated or edited to reduce its expressionor function can result in a shorter, semi-dwarf corn plant andphenotype.

Certain mutations of brachytic genes have been shown to result in ashort stature, semi-dwarf phenotype. In an aspect of the presentdisclosure, a corn plant is provided having a non-silent mutation oredit in a brachytic gene. See, e.g., PCT Application No.PCT/US2016/029492 and PCT/US2017/067888, the entire contents anddisclosures of which are incorporated herein by reference. Thus, ashorter corn plant can comprise a mutation (or edit) in a brachyticgene, and can be homozygous (or biallelic) for a mutation (or edit) in abrachytic gene. As used herein, a “brachytic mutant plant” refers to aplant having a short semi-dwarf height and stature relative to a controlplant (e.g., a wild-type sibling plant comprising all other traitsexcept the brachytic trait) due to a shortening of the average internodelength. Such a brachytic mutant plant can have a short semi-dwarf heightand stature due to a shortening of the average internode length. As usedherein, a “brachytic gene”, “BR gene” or “br gene”, or “Br gene” refersto any brachytic gene in a corn plant that when mutated or edited toreduce its expression or function can result in a shorter, semi-dwarfcorn plant and phenotype. In an aspect, an inbred corn plant orplurality of inbred corn plants provided herein each has a non-silentmutation or edit in a brachytic gene. In an aspect, the brachytic geneis a br1 mutant gene. In another aspect, the brachytic gene is a br2mutant gene. In yet another aspect, the brachytic gene is a br3 mutantgene.

In maize, brachytic mutants have a short stature due to a shortening ofthe internode length without a corresponding reduction in the number ofinternodes or the number and size of other organs, including the leaves,ear and tassel. See Kempton J. Hered. 11:111-115(1920); Pilu et al.,Molecular Breeding, 20:83-91(2007). Three brachytic mutants have beenisolated in maize to date: brachytic1 (br1), brachytic2 (br2) andbrachytic3 (br3). Both br1 and br3 mutations cause a reduction in cornplant height, which has been thought too severe for commercialexploitation due to potential impacts on yield. In contrast, the br2mutant has particular agronomic potential because of shortening of theinternodes of the lower stalk without an obvious reduction in otherplant organs. In addition, br2 lines exhibit an unusual stalk strengthand tolerance to wind lodging, while the leaves are often darker andpersist longer in the active green than those of the wild-type plants.The br2 phenotype is insensitive to treatment with gibberellins, auxins,brassinosteroids and cytokinins, suggesting that the biosynthesis ofthese hormones is not modified by the br2 mutation. Multani et al.identified the genomic sequence of the br2 gene (SEQ ID NO: 58) anddeposited it under GenBank Accession No. AY366085. See Multani et al.,Science, 302(5642)81-84 (2003). br2 was annotated to encode a putativeprotein similar to adenosine triphosphate (ATP)-binding cassettetransporters of the multidrug resistant (MDR) class of P-glycoproteins(PGPs). Pilu et al. reported a br2-23 allele having an 8-bp deletion inthe 3′ end of the br2 gene and claimed a direct relationship betweenthis deletion and the brachytic phenotype in their br2-23 plants. SeePilu et al., Molecular Breeding, 20:83-91(2007). Nevertheless, the useof brachytic mutations in corn has not been exploited commerciallypartly because of the severity of the available brachytic mutantalleles.

A wild-type genomic DNA sequence of the br2 locus from a referencegenome is provided in SEQ ID NO: 132. A wild-type cDNA sequence of thebr2 locus from a reference genome is provided in SEQ ID NO: 180. Awild-type amino acid sequence encoded by SEQ ID NO: 180 is provided inSEQ ID NO: 181.

For the br2 gene, SEQ ID NO: 132 provides 954 nucleotides upstream ofthe br2 5′-UTR; nucleotides 955-1000 correspond to the 5′-UTR;nucleotides 1001-1604 correspond to the first exon; nucleotides1605-1747 correspond to the first intron; nucleotides 1748-2384correspond to the second exon; nucleotides 2385-2473 correspond to thesecond intron; nucleotides 2474-2784 correspond to the third exon;nucleotides 2785-3410 correspond to the third intron; nucleotides3411-3640 correspond to the fourth exon; nucleotides 3641-5309correspond to the fourth intron; nucleotides 5310-7667 correspond to thefifth exon; and nucleotides 7668-8029 correspond to the 3′-UTR. SEQ IDNO: 132 also provides 638 nucleotides downstream of the end of the3′-UTR (nucleotides 8030-8667).

As used herein, a “brachytic allele” is an allele at a particulargenomic locus that confers, or contributes to, a brachytic or semi-dwarfphenotype, such as an allele of a brachytic gene that causes a brachyticor semi-dwarf phenotype, or alternatively, is an allele that allows forthe identification of plants that comprise a brachytic phenotype orplants that can give rise to progenies with a brachytic phenotype. Forexample, a brachytic allele of a marker can be a marker allele thatsegregates with a brachytic phenotype.

In some aspects, a brachytic, dwarf, or semi-dwarf corn plant comprisesa reduced level of br2 mRNA and/or protein, as compared to a controlcorn plant not having the brachytic allele. In other aspects, the cornplants or seeds comprise reduced Br2 protein activity compared to acontrol plant not having the brachytic allele. In some aspects, theheight of a brachytic, dwarf, or semi-dwarf plant comprising a brachyticallele at maturity is reduced by at least 10%, at least 20%, at least30%, at least 40%, at least 50%, at least 60%, or at least 70% comparedto a control plant not having a brachytic allele. In another aspect, theyield of a brachytic, dwarf, or semi-dwarf corn plant comprising abrachytic allele is equal to or more than the yield of a control plantnot having the brachytic allele. In an aspect, a brachytic, dwarf, orsemi-dwarf corn plant comprising a brachytic allele requires about 5%,10%, 15%, 20%, or 25% fewer heat units than a control plant not havingthe brachytic allele to reach anthesis. In an aspect, a brachytic,dwarf, or semi-dwarf corn plant is homozygous for a brachytic allele. Inanother aspect, a brachytic, dwarf, or semi-dwarf corn plant isheterozygous for a brachytic allele. In another aspect, a brachytic,dwarf, or semi-dwarf corn plant is a hybrid. In another aspect, abrachytic, dwarf, or semi-dwarf corn plant is an inbred, such as afemale inbred.

In an aspect, this disclosure provides brachytic, dwarf, or semi-dwarfcorn plants comprising a brachytic allele comprising one or moresequences selected from the group consisting of SEQ ID NOs: 59-85. Inanother aspect, a brachytic, dwarf, or semi-dwarf corn plant comprises asingle gene conversion of the br2 genomic region.

In an aspect, a brachytic, dwarf, or semi-dwarf corn plant comprises abrachytic allele at a polymorphic locus, wherein the polymorphic locusis associated with, or linked to, a marker selected from the groupconsisting of SEQ ID NOs: 86-131. In another aspect, a brachytic alleleat a polymorphic locus is within 20 cM (centimorgans), within 10 cM,within 5 cM, within 1 cM, or within 0.5 cM of a marker selected from thegroup consisting of SEQ ID NOs: 86-131. In another aspect, a brachyticallele is at a polymorphic locus within 20 cM, within 10 cM, within 5cM, within 1 cM, or within 0.5 cM of a marker selected from the groupconsisting of SEQ ID NOs: 90-117. In another aspect, a brachytic alleleis at a polymorphic locus within 20 cM, within 10 cM, within 5 cM,within 1 cM, or within 0.5 cM of a marker selected from the groupconsisting of SEQ ID NOs: 92 and 117.

In an aspect, a corn plant or plurality of corn plants provided herein,such as a female corn plant or inbred or a plurality or population offemale corn plants, can comprise at least one non-natural brachyticmutation, where the corn plant exhibits a semi-dwarf phenotype comparedto a control corn plant not comprising the at least one non-naturalbrachytic mutation when grown under comparable conditions. In anotheraspect, a corn plant provided herein can comprise at least onenon-natural brachytic mutation. In another aspect, a corn plant providedherein can comprise at least one non-natural brachytic mutant allele. Inanother aspect, a corn plant provided herein can comprise at least onenon-natural brachytic mutation and exhibits a semi-dwarf phenotype. Inanother aspect, a corn plant provided herein can comprise at least onenon-natural brachytic mutant allele and exhibit a semi-dwarf phenotype.In another aspect, a corn plant provided herein can comprise anon-naturally occurring mutation in a br gene reducing the activity ofthe br gene, where the mutation is not introduced via a transposon. Inanother aspect, a corn plant provided herein can comprise a mutation ina br2 locus or gene as compared to a wildtype br2 locus or gene. In anaspect, a corn plant provided herein is homozygous (or biallelic) for amutation in a br2 locus or gene as compared to a wildtype br2 locus orgene. In another aspect, a corn plant provided herein is heterozygousfor a mutation in a br2 locus or gene as compared to a wildtype br2locus or gene. In another aspect, a corn plant provided herein cancomprise a modified br2 gene with reduced activity, where the corn plantdoes not comprise a br2-23 brachytic allele or SNP5259. In anotheraspect, a corn plant provided herein can comprise a synthetic mutationin a br gene, reducing the activity of the br gene.

In an aspect, a corn plant or plurality of corn plants provided herein,such as a female corn plant or inbred or a plurality or population offemale corn plants, can each comprise a non-transgene or non-transposonmediated mutation in a br gene reducing the activity of the br gene. Inanother aspect, a corn plant provided herein can comprise a recessive,non-transgenic br mutant allele. In another aspect, a corn plantprovided herein can comprise a heterologous polynucleotide capable ofsuppressing expression of a br gene or an mRNA transcribed therefrom. Inanother aspect, a corn plant provided herein can comprise a heterologouspolynucleotide capable of suppressing expression of a br1 gene or anmRNA transcribed therefrom. In another aspect, a corn plant providedherein can comprise a heterologous polynucleotide capable of suppressingexpression of a br2 gene or an mRNA transcribed therefrom. In anotheraspect, a corn plant provided herein can comprise a heterologouspolynucleotide capable of suppressing expression of a br3 gene or anmRNA transcribed therefrom. Additional details about altering theexpression of br genes can be found in PCT Application No.PCT/US2016/029492 and PCT/US2017/067888, the entire contents anddisclosure of which are incorporated herein by reference.

In an aspect, this disclosure provides a mutant allele of an endogenousbr2 locus, where the mutant allele comprises a DNA segment inserted intothe endogenous br2 locus, wherein the DNA segment encodes an antisenseRNA that is at least 70%, at least 80%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, or 100% complementary to at least20, at least 30, at least 40, at least 50, at least 100, at least 200,at least 300, at least 400, at least 500, at least 600, at least 700, atleast 800, at least 900, or at least 1000 consecutive nucleotides of SEQID NOs: 132 or 180, and wherein the mutant allele of the endogenous br2locus produces an RNA transcript comprising the antisense RNA sequence.In an aspect, a mutant allele of an endogenous br2 locus furthercomprises deletion of at least one portion of the endogenous br2 locus.In an aspect, a “portion” of an endogenous br2 locus refers to at least1 nucleotide.

In an aspect, a modified corn plant, or plant part thereof, ishomozygous for a deletion within an endogenous br2 locus. In an aspect,a modified corn plant, or plant part thereof, is biallelic for a firstmutant allele and a second mutant allele each within an endogenous br2locus. In an aspect, a first mutant allele comprises a deletion and/oran inversion or antisense sequence. In an aspect, a second mutant allelecomprises a deletion and/or an inversion or antisense sequence. In anaspect, a modified corn plant, or plant part thereof, is heterozygousfor a deletion and/or an inversion or antisense sequence within anendogenous br2 locus.

In an aspect, a modified corn plant, or plant part thereof, ishomozygous for a mutant allele at an endogenous br2 locus. In an aspect,a modified corn plant, or plant part thereof, is biallelic for a firstmutant allele and a second mutant allele at an endogenous br2 locus. Inan aspect, a modified corn plant, or plant part thereof, is heterozygousfor a mutant allele at an endogenous br2 locus.

In an aspect, a deletion within an endogenous br2 locus comprisesbetween 1 nucleotide and 8667 nucleotides, between 1 nucleotide and 8000nucleotides, between 1 nucleotide and 7000 nucleotides, between 1nucleotide and 6000 nucleotides, between 1 nucleotide and 5000nucleotides, between 1 nucleotide and 4000 nucleotides, between 1nucleotide and 3000 nucleotides, between 1 nucleotide and 2000nucleotides, between 1 nucleotide and 1000 nucleotides, between 1nucleotide and 750 nucleotides, between 1 nucleotide and 500nucleotides, between 1 nucleotide and 250 nucleotides, between 1nucleotide and 100 nucleotides, between 1 nucleotide and 50 nucleotides,between 10 nucleotide and 8000 nucleotides, between 10 nucleotide and5000 nucleotides, between 10 nucleotide and 2500 nucleotides, between 10nucleotide and 1000 nucleotides, between 10 nucleotide and 100nucleotides, between 100 nucleotide and 8000 nucleotides, between 100nucleotide and 5000 nucleotides, between 100 nucleotide and 2500nucleotides, between 100 nucleotide and 1000 nucleotides, or between 100nucleotide and 500 nucleotides. In an aspect, a deletion within anendogenous br2 locus comprises at least 1 nucleotide. In an aspect, adeletion within an endogenous br2 locus comprises at least 2nucleotides. In an aspect, a deletion within an endogenous br2 locuscomprises at least 5 nucleotides. In an aspect, a deletion within anendogenous br2 locus comprises at least 10 nucleotides. In an aspect, adeletion within an endogenous br2 locus comprises at least 20nucleotides. In an aspect, a deletion within an endogenous br2 locuscomprises at least 30 nucleotides. In an aspect, a deletion within anendogenous br2 locus comprises at least 40 nucleotides. In an aspect, adeletion within an endogenous br2 locus comprises at least 50nucleotides. In an aspect, a deletion within an endogenous br2 locuscomprises at least 100 nucleotides. In an aspect, a deletion within anendogenous br2 locus comprises at least 200 nucleotides. In an aspect, adeletion within an endogenous br2 locus comprises at least 300nucleotides. In an aspect, a deletion within an endogenous br2 locuscomprises at least 400 nucleotides. In an aspect, a deletion within anendogenous br2 locus comprises at least 500 nucleotides.

In an aspect, this disclosure provides a mutant allele of an endogenousbr2 locus, where the mutant allele comprises a deletion of at least onenucleotide from at least one exon of an endogenous br2 locus as comparedto SEQ ID NO: 132. In an aspect, a deletion further comprises thedeletion of at least one exon of an endogenous br2 locus as compared toSEQ ID NO: 132. In an aspect, a deletion comprises the deletion of anendogenous br2 locus. In an aspect, a deletion comprises the deletion ofat least two exons from an endogenous br2 locus. In an aspect, twodeleted exons from an endogenous br2 locus are contiguous. In an aspect,two deleted exons from an endogenous br2 locus are not contiguous. In anaspect, the first exon of an endogenous br2 locus is deleted. In anaspect, the second exon of an endogenous br2 locus is deleted. In anaspect, the third exon of an endogenous br2 locus is deleted. In anaspect, the fourth exon of an endogenous br2 locus is deleted. In anaspect, the fifth exon of an endogenous br2 locus is deleted. In anaspect, a deletion further comprises the deletion of at least onenucleotide from at least one intron of an endogenous br2 locus. In anaspect, a deletion further comprises the deletion of at least onenucleotide from at least one intron of an endogenous br2 locus. In anaspect, a deletion comprises the deletion of at least one intron of anendogenous br2 locus. In an aspect, a deletion comprises the deletion ofat least one nucleotide of the 5′-untranslated region of the endogenousbr2 locus. In an aspect, a deletion comprises the deletion of at leastone nucleotide of the 3′-untranslated region of the endogenous br2locus.

In an aspect, a deletion comprises deletion of at least one nucleotideof the first exon of an endogenous br2 locus. In an aspect, a deletioncomprises deletion of at least one nucleotide of the second exon of anendogenous br2 locus. In an aspect, a deletion comprises deletion of atleast one nucleotide of the third exon of an endogenous br2 locus. In anaspect, a deletion comprises deletion of at least one nucleotide of thefourth exon of an endogenous br2 locus. In an aspect, a deletioncomprises deletion of at least one nucleotide of the fifth exon of anendogenous br2 locus.

In an aspect, a deletion comprises deletion of the first exon of anendogenous br2 locus. In an aspect, a deletion comprises deletion of thesecond exon of an endogenous br2 locus. In an aspect, a deletioncomprises deletion of the third exon of an endogenous br2 locus. In anaspect, a deletion comprises deletion of the fourth exon of anendogenous br2 locus. In an aspect, a deletion comprises deletion of thefifth exon of an endogenous br2 locus.

In an aspect, a deletion comprises deletion of at least one nucleotideof at least one intron of an endogenous br2 locus. In an aspect, adeletion comprises deletion of at least one intron of an endogenous br2locus. In an aspect, a deletion comprises deletion of at least onenucleotide of the 5′-untranslated region of an endogenous br2 locus. Inan aspect, a deletion comprises deletion of the 5′-untranslated regionof an endogenous br2 locus. In an aspect, a deletion comprises deletionof at least one nucleotide of the 3′-untranslated region of anendogenous br2 locus. In an aspect, a deletion comprises deletion of the3′-untranslated region of an endogenous br2 locus.

In an aspect, a deletion comprises a deletion of at least one nucleotideof at least one intron, a deletion of at least one nucleotide of atleast one exon, at least one nucleotide of a 5′-untranslated region(UTR), at least one nucleotide of a 3′-UTR, or any combination thereofof an endogenous br2 locus.

In an aspect, a deletion comprises deletion of at least one nucleotidefrom a first exon and at least one nucleotide from a second exon of anendogenous br2 locus. In an aspect, a deletion comprises deletion of atleast one nucleotide from a first exon, at least one nucleotide from asecond exon, and at least one nucleotide from a third exon of anendogenous br2 locus. In an aspect, a deletion comprises deletion of atleast one nucleotide from a first exon, at least one nucleotide from asecond exon, at least one nucleotide from a third exon, and at least onenucleotide from a fourth exon of an endogenous br2 locus. In an aspect,a deletion comprises deletion of at least one nucleotide from a firstexon, at least one nucleotide from a second exon, at least onenucleotide from a third exon, at least one nucleotide from a fourthexon, and at least one nucleotide from a fifth exon of an endogenous br2locus.

In an aspect, a deletion comprises a deletion of a first exon and asecond exon from an endogenous br2 locus. In an aspect, a first deletedexon and a second deleted exon are contiguous. In an aspect, a firstdeleted exon and a second deleted exon are not contiguous. In an aspect,a deletion comprises deletion of a first exon and a second exon from anendogenous br2 locus. In an aspect, a deletion comprises deletion of afirst exon, a second exon, and a third exon from an endogenous br2locus. In an aspect, a deletion comprises deletion of a first exon, asecond exon, a third exon, and a fourth exon from an endogenous br2locus. In an aspect, a deletion comprises deletion of a first exon, asecond exon, a third exon, a fourth exon, and a fifth exon from anendogenous br2 locus.

In an aspect, a deletion in an endogenous br2 locus results in apremature stop codon within an mRNA transcript encoding a Br2 protein.In an aspect, this disclosure provides a modified corn plant, or plantpart thereof, comprising a premature stop codon within a nucleic acidsequence encoding a Brachytic2 protein as compared to a control cornplant or plant part thereof. In an aspect, a mutant allele encodes anmRNA transcript comprising a premature stop codon as compared to SEQ IDNO: 180.

According to some embodiments, an endogenous gene can be edited orengineered to express a truncated protein relative to a wild typeprotein by the introduction of a premature stop codon into the codingsequence and the encoded mRNA transcript of the endogenous gene. Withoutbeing bound by theory, a truncated Br2 protein expressed from an editedendogenous br2 gene comprising a premature stop codon may not only benon-functional or have reduced function, but also interfere with thefunctioning of a wild type Br2 protein to act in a dominant orsemi-dominant manner. In an aspect, a premature stop codon within anmRNA transcript results in translation of a truncated protein ascompared to a control mRNA transcript that lacks the premature stopcodon. As used herein, a “stop codon” refers to a nucleotide tripletwithin an mRNA transcript that signals a termination of proteintranslation. A “premature stop codon” refers to a stop codon positionedearlier (e.g., on the 5′-side) than the normal stop codon position in anendogenous mRNA transcript. A stop codon is a nucleotide triplet in amRNA that signals the termination of protein translation from the mRNA.Without being limiting, several stop codons are known in the art,including “UAG,” “UAA,” “UGA,” “TAG,” “TAA,” and “TGA.” In an aspect, apremature stop codon can arise from a frameshift mutation. Frameshiftmutations can be caused by the insertion or deletion of one or morenucleotides in a protein-coding sequence. In an aspect, a premature stopcodon can arise from a substitution, missense or nonsense mutation. Inan aspect, a nonsense, missense or frameshift mutation provided hereinis located in an exon of a br2 gene. In an aspect, a substitution,insertion or deletion provided herein is located in a gene elementselected from the group consisting of an exon and an intron/exon splicesite. A substitution, insertion or deletion provided herein can generatea protein with one or more, two or more, three or more, four or more,five or more, six or more, seven or more, eight or more, nine or more,or ten or more nonsense mutations. According to present embodiments, apremature stop codon may be introduced into the coding sequence of anendogenous br2 gene via a targeted editing technique and/orsite-directed integration. The premature stop codon may be generated viaimperfect DNA repair following a double strand break introduced into abr2 gene, or via template-assisted repair following introduction of thedouble strand break using a DNA donor template comprising the prematurestop codon. Such a DNA donor template may further comprise one or moreflanking homologous arms or sequences that are identical, homologous orcomplementary to a corresponding sequence of the endogenous br2 gene tohelp promote recombination between the donor template and the targetsite in the endogenous br2 gene for insertion of a sequence comprisingthe premature stop codon at the desired target site.

In an aspect, a premature stop codon is positioned within the first exonof an endogenous br2 locus. In an aspect, a premature stop codon ispositioned within the second exon of an endogenous br2 locus. In anaspect, a premature stop codon is positioned within the third exon of anendogenous br2 locus. In an aspect, a premature stop codon is positionedwithin the fourth exon of an endogenous br2 locus. In an aspect, apremature stop codon is positioned within the fifth exon of anendogenous br2 locus.

In an aspect, a mutant allele provided herein encodes a truncatedprotein as compared to SEQ ID NO: 181. As used herein, a “truncated”protein or polypeptide comprises at least one fewer amino acid ascompared to an endogenous control protein or polypeptide. For example,if endogenous Protein A comprises 100 amino acids, a truncated versionof Protein A can comprise between 1 and 99 amino acids.

In an aspect, this disclosure provides a modified corn plant, or plantpart thereof, comprising a premature stop codon within a nucleic acidsequence encoding a Brachytic2 protein as compared to a nucleic acidsequence of a control corn plant or plant part thereof. In an aspect,this disclosure provides a modified corn plant, or plant part thereof,comprising a premature stop codon within a nucleic acid sequenceencoding a Brachytic2 protein. In an aspect, this disclosure provides amodified corn plant, or plant part thereof, comprising a truncatedBrachytic2 protein encoded by a nucleic acid sequence comprising apremature stop codon as compared to a wildtype or control nucleic acidsequence. In an aspect, this disclosure provides a modified corn plant,or plant part thereof, comprising a premature stop codon in a nucleicacid sequence as compared to SEQ ID NO: 180.

In an aspect, a premature stop codon is positioned within a region of abr2 mRNA transcript selected from the group consisting of the firstexon, the second exon, the third exon, the fourth exon, and the fifthexon.

In an aspect, a truncated Br2 protein sequence comprises fewer than 1378amino acids. In an aspect, a truncated Br2 protein sequence comprisesfewer than 1375 amino acids. In an aspect, a truncated Br2 proteinsequence comprises fewer than 1350 amino acids. In an aspect, atruncated Br2 protein sequence comprises fewer than 1300 amino acids. Inan aspect, a truncated Br2 protein sequence comprises fewer than 1200amino acids. In an aspect, a truncated Br2 protein sequence comprisesfewer than 1100 amino acids. In an aspect, a truncated Br2 proteinsequence comprises fewer than 1000 amino acids. In an aspect, atruncated Br2 protein sequence comprises fewer than 900 amino acids. Inan aspect, a truncated Br2 protein sequence comprises fewer than 800amino acids. In an aspect, a truncated Br2 protein sequence comprisesfewer than 700 amino acids. In an aspect, a truncated Br2 proteinsequence comprises fewer than 600 amino acids. In an aspect, a truncatedBr2 protein sequence comprises fewer than 500 amino acids. In an aspect,a truncated Br2 protein sequence comprises fewer than 400 amino acids.In an aspect, a truncated Br2 protein sequence comprises fewer than 300amino acids. In an aspect, a truncated Br2 protein sequence comprisesfewer than 200 amino acids. In an aspect, a truncated Br2 proteinsequence comprises fewer than 100 amino acids. In an aspect, a truncatedBr2 protein sequence comprises fewer than 50 amino acids.

In an aspect, a truncated Br2 protein sequence comprises between 1 aminoacid and 1378 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 25 amino acids and 1378 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 50 aminoacids and 1378 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 100 amino acids and 1378 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 250 aminoacids and 1378 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 500 amino acids and 1378 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 750 aminoacids and 1378 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 1000 amino acids and 1378 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 1250 aminoacids and 1378 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 100 amino acids and 1000 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 250 aminoacids and 1000 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 500 amino acids and 1000 amino acids. In anaspect, a truncated Br2 protein sequence comprises between 750 aminoacids and 1000 amino acids. In an aspect, a truncated Br2 proteinsequence comprises between 1000 amino acids and 1378 amino acids.

In an aspect, a mutant allele of an endogenous br2 locus suppresses theexpression of a wild-type allele of the endogenous br2 locus.

In an aspect, an RNA transcript comprises one or more sequence elementsof the endogenous br2 locus selected from the group consisting of5′-UTR, first exon, first intron, second exon, second intron, thirdexon, third intron, fourth exon, fourth intron, fifth exon, 3′-UTR, andany portion thereof. In an aspect, an endogenous sequence of an RNAtranscript is at least 85%, at least 90%, at least 91%, at least 92%, atleast 93%, at least 94%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, or 100% identical to at least 20, at least 30,at least 40, at least 50, at least 100, at least 200, at least 300, atleast 400, at least 500, at least 600, at least 700, at least 800, atleast 900, or at least 1000 consecutive nucleotides of SEQ ID NOs: 132or 180.

In an aspect, a DNA segment comprises a nucleotide sequence originatingfrom the endogenous br2 locus. In an aspect, a DNA segment comprises aninverted genomic fragment of the endogenous br2 locus. In an aspect, aDNA segment is inserted near or adjacent to a corresponding endogenousDNA segment of an endogenous br2 locus. In an aspect, a DNA segment isinserted within a region selected from the group consisting of the 5′untranslated region (UTR), first exon, first intron, second exon, secondintron, third exon, third intron, fourth exon, fourth intron, fifthexon, and 3′ UTR of an endogenous br2 locus, and a combination thereof.In an aspect, the sense strand of a DNA segment comprises a sequencethat is at least 70%, at least 80%, at least 85%, at least 90%, at least91%, at least 92%, at least 93%, at least 94%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, or 100% identical orcomplementary to an exon sequence of an endogenous br2 locus. In anaspect, the sense strand of a DNA segment comprises a sequence at least70%, at least 80%, at least 85%, at least 90%, at least 91%, at least92%, at least 93%, at least 94%, at least 95%, at least 96%, at least97%, at least 98%, at least 99%, or 100% identical or complementary toan untranslated region (UTR) sequence of the endogenous br2 locus. In anaspect, the sense strand of a DNA segment comprises a sequence at least70%, at least 80%, at least 85%, at least 90%, at least 91%, at least92%, at least 93%, at least 94%, at least 95%, at least 96%, at least97%, at least 98%, at least 99%, or 100% identical or complementary toan exon sequence and an intron sequence of the endogenous br2 locus, theexon sequence and the intron sequence being contiguous within theendogenous locus. In an aspect, a DNA segment comprises a sequencehaving at least 70%, at least 80%, at least 85%, at least 90%, at least91%, at least 92%, at least 93%, at least 94%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, or 100% identical orcomplementary to SEQ ID NOs: 132 or 180.

In an aspect, a mutant allele encodes a truncated Br2 protein ascompared to SEQ ID NO: 181. In an aspect, a truncated Br2 protein is atleast 1, at least 2, at least 3, at least 4, at least 5, at least 10, atleast 20, at least 30, at least 40, at least 50, at least 60, at least70, at least 80, at least 90, at least 100, or at least 150 amino acidsshorter than the amino acid sequence of SEQ ID NO: 181.

In an aspect, an intervening DNA sequence comprises a native sequence ofan endogenous br2 locus. In an aspect, an intervening DNA sequencecomprises an exogenous sequence inserted into an endogenous br2 locus.

Corn plants or seeds can be screened and/or selected for the presence ofmutations, edits, or transgenes using any methodologies known to thosehaving ordinary skill in the art. Examples of screening and selectionmethodologies include, but are not limited to, Southern analysis, PCRamplification for detection of a polynucleotide, Northern blots, RNaseprotection, primer-extension, RT-PCR amplification for detecting RNAtranscripts, Sanger sequencing, Next Generation sequencing technologies(e.g., Illumina, PacBio, Ion Torrent, 454) enzymatic assays fordetecting enzyme or ribozyme activity of polypeptides andpolynucleotides, and protein gel electrophoresis, Western blots,immunoprecipitation, and enzyme-linked immunoassays to detectpolypeptides. Other techniques such as in situ hybridization, enzymestaining, and immunostaining also can be used to detect the presence orexpression of polypeptides and/or polynucleotides. Methods forperforming all of the referenced techniques are known.

In an aspect, selecting is performed following genotyping seeds. In anaspect, selecting is performed following phenotypic analysis. In anaspect, selecting is performed following germination of the seeds. In anaspect, selecting is performed after determining the zygosity of theseed. In an aspect, selecting comprises a visual assay of the seed. Inan aspect, selecting comprises separating seeds. In an aspect, selectingcomprises placing selected seeds in a container or packet.

Without being limiting, this disclosure provides several methods relatedto the harvesting of corn plants.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a DNAsegment inserted into the endogenous br2 locus, wherein the DNA segmentencodes an antisense RNA that is at least 70% complementary to at least20 consecutive nucleotides of SEQ ID NO: 132 or 180, and wherein themutant allele of the endogenous br2 locus produces an RNA transcriptcomprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a deletionof at least one nucleotide from an endogenous br2 locus as compared toSEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a premature stop codon within anucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous br2 locus, wherein the DNAsegment encodes an antisense RNA that is at least 70% complementary toat least 20 consecutive nucleotides of SEQ ID NO: 132 or 180, andwherein the mutant allele of the endogenous br2 locus produces an RNAtranscript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises adeletion of at least one nucleotide from an endogenous br2 locus ascompared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a premature stop codon withina nucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days afterfertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein average kernelmoisture content is less than or equal to 30%, and wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a DNAsegment inserted into the endogenous br2 locus, wherein the DNA segmentencodes an antisense RNA that is at least 70% complementary to at least20 consecutive nucleotides of SEQ ID NO: 132 or 180, and wherein themutant allele of the endogenous br2 locus produces an RNA transcriptcomprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of an endogenousBrachytic2 (br2) locus, wherein the mutant allele comprises a deletionof at least one nucleotide from an endogenous br2 locus as compared toSEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a premature stop codon within anucleic acid sequence encoding a Brachytic2 protein as compared to acontrol corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after atfertilization or silking of at least 50% of said plurality of cornplants, wherein the average yield of said field is at least 170 bushelsper acre, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_3 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the DNA segment encodes anantisense RNA sequence that is at least 70% complementary to at least 20consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a DNA segment inserted into theendogenous br2 locus, wherein the DNA segment encodes an antisense RNAthat is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of an endogenous Brachytic2 (br2) locus,wherein the mutant allele comprises a deletion of at least onenucleotide from an endogenous br2 locus as compared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a dominant or semi-dominant transgene or mutant allele of agene, and wherein the transgene or mutant allele causes a short staturephenotype in the at least one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a premature stop codon within a nucleic acid sequence encodinga Brachytic2 protein as compared to a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anyportion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any portion thereof, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification which resultsin the transcription of an antisense strand of at least an exon, anintron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a sequence selected from the groupconsisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, andany complementary sequence thereof, and any portion of the foregoing, ofthe endogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene; wherein the first sequenceand the second sequence are contiguous or separated only by anintervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic deletion relative to awild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5thintron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein the average yieldof said field is at least 170 bushels per acre, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genomic sequence comprising afirst sequence and a second sequence; wherein the first sequencecomprises at least 15 consecutive nucleotides of one or more of SEQ IDNOs: 228-235 and 276-283; wherein the second sequence comprises at least15 consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_3 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_3 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_3 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous GA20 oxidase_5 locus, whereinthe DNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises aDNA segment inserted into the endogenous br2 locus, wherein the DNAsegment encodes an antisense RNA that is at least 70% complementary toat least 20 consecutive nucleotides of SEQ ID NO: 132 or 180, andwherein the mutant allele of the endogenous br2 locus produces an RNAtranscript comprising the antisense RNA sequence.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of anendogenous Brachytic2 (br2) locus, wherein the mutant allele comprises adeletion of at least one nucleotide from an endogenous br2 locus ascompared to SEQ ID NO: 132.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a dominant orsemi-dominant transgene or mutant allele of a gene, and wherein thetransgene or mutant allele causes a short stature phenotype in the atleast one corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a premature stop codonwithin a nucleic acid sequence encoding a Brachytic2 protein as comparedto a control corn plant.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of thetranscription termination sequence of the endogenous Zm.SAMT gene, andwherein the mutant allele produces a RNA molecule comprising anantisense sequence complementary to all or part of the sense strand ofthe endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any portion thereof, of the endogenousZm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises asequence selected from the group consisting of SEQ ID NOs: 87-105.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises afirst sequence and a second sequence; wherein the first sequencecomprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2ndintron, 3rd exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR, 1stexon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon,4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene; whereinthe first sequence and the second sequence are contiguous or separatedonly by an intervening sequence of fewer than 555 nucleotides.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rdexon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1st exon, 1stintron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4thintron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7thintron, 8th exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.SAMT gene.

In an aspect, this disclosure provides a method comprising harvesting aplurality of corn plants from a field at least 1 day after the averagekernel moisture content of at least 50% of said plurality of corn plantsis between 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises a mutant allele of theendogenous GA20 oxidase_5 locus, wherein the mutant allele comprises agenomic sequence comprising a first sequence and a second sequence;wherein the first sequence comprises at least 15 consecutive nucleotidesof one or more of SEQ ID NOs: 228-235 and 276-283; wherein the secondsequence comprises at least 15 consecutive nucleotides of one or more ofSEQ ID NOs: 235-276; and wherein the genomic sequence is at least 50consecutive nucleotides in length, and/or fewer than 9000 consecutivenucleotides in length.

In an aspect, at least 10% of the corn plants in a field are brachyticcorn plants. In an aspect, at least 20% of the corn plants in a fieldare brachytic corn plants. In an aspect, at least 30% of the corn plantsin a field are brachytic corn plants. In an aspect, at least 40% of thecorn plants in a field are brachytic corn plants. In an aspect, at least50% of the corn plants in a field are brachytic corn plants. In anaspect, at least 60% of the corn plants in a field are brachytic cornplants. In an aspect, at least 70% of the corn plants in a field arebrachytic corn plants. In an aspect, at least 80% of the corn plants ina field are brachytic corn plants. In an aspect, at least 90% of thecorn plants in a field are brachytic corn plants. In an aspect, 100% ofthe corn plants in a field are brachytic corn plants.

In an aspect, between 1% and 100% of the corn plants in a field arebrachytic corn plants. In an aspect, between 10% and 100% of the cornplants in a field are brachytic corn plants. In an aspect, between 20%and 100% of the corn plants in a field are brachytic corn plants. In anaspect, between 30% and 100% of the corn plants in a field are brachyticcorn plants. In an aspect, between 40% and 100% of the corn plants in afield are brachytic corn plants. In an aspect, between 50% and 100% ofthe corn plants in a field are brachytic corn plants. In an aspect,between 60% and 100% of the corn plants in a field are brachytic cornplants. In an aspect, between 70% and 100% of the corn plants in a fieldare brachytic corn plants. In an aspect, between 80% and 100% of thecorn plants in a field are brachytic corn plants. In an aspect, between90% and 100% of the corn plants in a field are brachytic corn plants.

It will be appreciated in the art that dwarf, semi-dwarf, and brachyticplants can be inbred or hybrid plants.

Three brachytic mutants have been isolated in maize to date: brachytic1(br1), brachytic2 (br2) and brachytic3 (br3). brachytic3 is also knownas brevis plant 1 (Bv1). Both br1 and br3 mutations cause a reduction incorn plant height which has been thought too severe for commercialexploitation due to potential impacts on yield. In contrast, the br2mutant has particular agronomic potential because of shortening of theinternodes of the lower stalk without an obvious reduction in otherplant organs. In addition, br2 lines exhibit an unusual stalk strengthand tolerance to wind lodging, while the leaves are often darker andpersist longer in the active green than those of the wild-type plants.The br2 phenotype is insensitive to treatment with Gibberellins, auxins,brassinosteroids and cytokinins, suggesting that the biosynthesis ofthese hormones is not modified by the br2 mutation. Multani et al.identified the genomic sequence of the br2 gene and deposited it underGenBank Accession No. AY366085. See Multani et al., Science, 302:81-84(2003). Br2 was annotated to encode a putative protein similar toadenosine triphosphate (ATP)-binding cassette transporters of themultidrug resistant (MDR) class of P-glycoproteins (PGPs). Pilu et al.reported a br2-23 allele having an 8-bp deletion in the 3′ end of thebr2 gene and claimed a direct relationship between this deletion and thebrachytic phenotype in their br2-23 plants. See Pilu et al., MolecularBreeding, 20:83-91(2007). Other brachytic gene alleles are known in theart which may be used according to embodiments of the presentdisclosure.

In some aspects, a brachytic, dwarf, or semi-dwarf corn plant comprisesa reduced level of Br2 mRNA and/or protein compared to a control cornplant not having the brachytic allele. In other aspects, the corn plantsor seeds comprise reduced Br2 protein activity compared to a controlplant not having the brachytic allele. In some aspects, the height of abrachytic, dwarf, or semi-dwarf plant comprising a brachytic allele atmaturity is reduced by at least 5%, at least 10%, at least 20%, at least30%, at least 40%, at least 50%, at least 60%, or at least 70% comparedto a control plant not having a brachytic allele. In another aspect, theyield of a brachytic, dwarf, or semi-dwarf corn plant comprising abrachytic allele is equal to or more than the yield of a control plantnot having the brachytic allele.

In an aspect, at least 10% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, at least 20% of the corn plants in a field comprise a mutationin a br2 locus as compared to a wildtype br2 locus. In an aspect, atleast 30% of the corn plants in a field comprise a mutation in a br2locus as compared to a wildtype br2 locus. In an aspect, at least 40% ofthe corn plants in a field comprise a mutation in a br2 locus ascompared to a wildtype br2 locus. In an aspect, at least 50% of the cornplants in a field comprise a mutation in a br2 locus as compared to awildtype br2 locus. In an aspect, at least 60% of the corn plants in afield comprise a mutation in a br2 locus as compared to a wildtype br2locus. In an aspect, at least 70% of the corn plants in a field comprisea mutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, at least 80% of the corn plants in a field comprise a mutationin a br2 locus as compared to a wildtype br2 locus. In an aspect, atleast 90% of the corn plants in a field comprise a mutation in a br2locus as compared to a wildtype br2 locus. In an aspect, 100% of thecorn plants in a field comprise a mutation in a br2 locus as compared toa wildtype br2 locus.

In an aspect, between 1% and 100% of the corn plants in a field comprisea mutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 10% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 20% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 30% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 40% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 50% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 60% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 70% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 80% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus. In anaspect, between 90% and 100% of the corn plants in a field comprise amutation in a br2 locus as compared to a wildtype br2 locus.

In another aspect, a corn plant provided herein comprises anon-transgene or non-transposon mediated mutation in a Br gene reducingthe activity of the Br gene. In a further aspect, a corn plant providedherein comprises a recessive, non-transgenic Br mutant allele. Inanother aspect, a corn plant provided herein comprises a heterologouspolynucleotide capable of suppressing expression of a Br gene or an mRNAtranscribed therefrom. In another aspect, a corn plant provided hereincomprises a heterologous polynucleotide capable of suppressingexpression of a Br1 gene or an mRNA transcribed therefrom. In anotheraspect, a corn plant provided herein comprises a heterologouspolynucleotide capable of suppressing expression of a Br2 gene or anmRNA transcribed therefrom. In another aspect, a corn plant providedherein comprises a heterologous polynucleotide capable of suppressingexpression of a Br3 gene or an mRNA transcribed therefrom. Additionaldetails about corn plants and altering the expression of Br genes can befound in PCT Application No. PCT/US2016/029492 and PCT/US2017/067888,which are incorporated herein by reference in their entirety.

In an aspect, a mutant allele of an endogenous br2 locus suppresses theexpression of a wild-type allele of the endogenous br2 locus. In anaspect, a mutant allele product of an endogenous br2 locus disrupts thefunction of a wild-type allele product of the endogenous br2 locus. Inan aspect, a “product” of a mutant allele is a mRNA transcript. In anaspect, a “product” of a mutant allele comprises an antisense RNA. In anaspect, a “product” of a mutant allele is a protein.

In an aspect, at least 10% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, at least 20% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, at least 30% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, at least 40% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, at least 50% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, at least 60% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, at least 70% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, at least 80% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, at least 90% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, 100% of the cornplants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.

In an aspect, between 1% and 100% of the corn plants in a field comprisea heterologous polynucleotide capable of suppressing expression of a br2gene or an mRNA transcribed therefrom. In an aspect, between 10% and100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a br2 gene or anmRNA transcribed therefrom. In an aspect, between 20% and 100% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, between 30% and 100% of the corn plants in a fieldcomprise a heterologous polynucleotide capable of suppressing expressionof a br2 gene or an mRNA transcribed therefrom. In an aspect, between40% and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a br2 gene or anmRNA transcribed therefrom. In an aspect, between 50% and 100% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, between 60% and 100% of the corn plants in a fieldcomprise a heterologous polynucleotide capable of suppressing expressionof a br2 gene or an mRNA transcribed therefrom. In an aspect, between70% and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a br2 gene or anmRNA transcribed therefrom. In an aspect, between 80% and 100% of thecorn plants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a br2 gene or an mRNA transcribed therefrom.In an aspect, between 90% and 100% of the corn plants in a fieldcomprise a heterologous polynucleotide capable of suppressing expressionof a br2 gene or an mRNA transcribed therefrom.

Active or bioactive gibberellic acids (i.e., “active gibberellins” or“active GAs”) are known in the art for a given plant species, asdistinguished from inactive GAs. For example, active GAs in corn andhigher plants include the following: GA1, GA3, GA4, and GA7. Thus, an“active GA-producing tissue” is a plant tissue that produces one or moreactive GAs. In an aspect, a modified corn plant comprises a level of oneor more active GAs in at least one internode tissue of a stem or stalkthat is at least 1%, at least 2%, at least 2.5%, at least 3%, at least4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, atleast 10%, at least 11%, at least 12%, at least 13%, at least 14%, atleast 15%, at least 20%, at least 25%, at least 30%, at least 35%, atleast 40%, at least 45%, or at least 50% lower than the level of the oneor more active GAs in the same internode tissue of an unmodified controlplant. In an aspect, a modified corn plant provided herein comprises alower level of one or more active GAs in at least one internode tissueof a stem or stalk as compared to the same internode tissue of anunmodified control plant.

Certain biosynthetic enzymes (e.g., GA20 oxidase and GA3 oxidase) andcatabolic enzymes (e.g., GA2 oxidase) in the GA pathway participate inGA synthesis and degradation, respectively, to affect active GA levelsin plant tissues. Thus, in addition to suppression of certain GA20oxidase genes, it is further proposed that suppression of a GA3 oxidasegene in a constitutive or tissue-specific or tissue-preferred manner canalso produce corn plants having a short stature phenotype and increasedlodging resistance, with possible increased yield, but without off-typesin the ear.

Without being bound by theory, it is proposed that suppression of GA20or GA3 oxidase gene(s) and/or targeting of a subset of one or more GAoxidase gene(s) can be effective in achieving a short stature,semi-dwarf phenotype with increased resistance to lodging, but withoutreproductive off-types in the ear. It is further proposed, without beinglimited by theory, that suppression of GA20 and/or GA3 oxidase gene(s)through constitutive expression or in active GA-producing tissues, suchas the vascular and/or leaf tissues of the plant, can produce ashort-stature plant with increased lodging resistance, but withoutsignificant off-types in reproductive tissues. Expression of a GA20 orGA3 oxidase suppression element using a constitutive, vascular and/orleaf promoter can be sufficient and effective at producing plants withthe short stature phenotype, while avoiding potential off-types inreproductive tissues that were previously observed with GA mutants incorn. For example, GA20 and/or GA3 oxidase gene(s) can be targeted forsuppression using a vascular promoter, such as a rice tungro bacilliformvirus (RTBV) promoter, that drives expression in vascular tissues ofplants. The expression pattern of the RTBV promoter is enriched invascular tissues of corn plants relative to non-vascular tissues, whichis sufficient to produce a semi-dwarf phenotype in corn plants whenoperably linked to a suppression element targeting GA20 and GA3 oxidasegene(s). Lowering of active GA levels in tissue(s) of a corn plant, suchas in the stalk, stem, or internode(s) of corn plant, that produceactive GAs can reduce plant height and increase lodging resistance, andavoid off-types in the reproductive tissues of the plant, such as in thefemale (ear) or male (tassel) tissues of the plant.

Without being limited by theory, it is proposed that short stature,semi-dwarf phenotypes in corn plants can result from a sufficient levelof expression of a suppression construct targeting certain GA oxidasegene(s) in active GA-producing tissue(s) of the plant. For targetedsuppression of certain GA20 oxidase genes in corn, restricting thepattern of expression to avoid reproductive ear tissues may not benecessary to avoid reproductive off-types in the developing ear.However, expression of a GA20 oxidase suppression construct at lowlevels, and/or in a limited number of plant tissues, can be insufficientto cause a significant short stature, semi-dwarf phenotype. Given thatthe observed semi-dwarf phenotype with targeted GA20 oxidase suppressionis the result of shortening the stem internodes of the plant,suppression of GA20 oxidase genes in at least some stem tissues was notsufficient to cause shortening of the internodes and reduced plantheight. Without being bound by theory, it is proposed that suppressionof certain GA oxidase gene(s) in tissue(s) and/or cell(s) of the plantwhere active GAs are produced, and not necessarily in stem or internodetissue(s), can be sufficient to produce semi-dwarf plants, even thoughthe short stature trait is due to shortening of the stem internodes.Given that GAs can migrate through the vasculature of the plant,manipulating GA oxidase genes in plant tissue(s) where active GAs areproduced can result in a short stature, semi-dwarf plant, even thoughthis may be largely achieved by suppressing the level of active GAsproduced in non-stem tissues (i.e., away from the site of action in thestem where reduced internode elongation leads to the semi-dwarfphenotype). Indeed, suppression of certain GA20 oxidase genes in leaftissues causes a moderate semi-dwarf phenotype in corn plants. Giventhat expression of a GA20 oxidase suppression construct with severaldifferent “stem” promoters did not produce the semi-dwarf phenotype incorn, it is noteworthy that expression of the same GA20 oxidasesuppression construct with a vascular promoter was effective atconsistently producing the semi-dwarf phenotype with a high degree ofpenetrance across events and germplasms. A semi-dwarf phenotype was alsoobserved with expression of the same GA20 oxidase suppression constructusing other vascular promoters and with various constitutive promoterswithout any observable off-types. Additional details about corn plantsand altering the expression of GA20 oxidase and GA3 oxidase genes can befound in PCT Application No. PCT/US2017/047405 and PCT/US2019/018133,which are incorporated herein by reference in their entirety.

In an aspect, a corn plant or plurality of corn plants provided hereincan each comprise a recombinant DNA construct or polynucleotidesequence, where the recombinant DNA construct or polynucleotide sequencecomprises a transcribable DNA sequence encoding a non-coding RNAmolecule that targets at least one endogenous GA20 or GA3 oxidase genefor suppression. In another aspect, a corn plant provided herein cancomprise suppressed GA3 oxidase gene expression in one or more tissuesas compared to a wild-type control plant. In another aspect, a cornplant provided herein can comprise suppressed GA20 oxidase geneexpression in one or more tissues as compared to a wild-type controlplant. In another aspect, a corn plant provided herein can comprise amutation at or near an endogenous GA oxidase gene, where the expressionlevel of the endogenous GA oxidase gene is reduced or eliminated in thecorn plant, and where the corn plant has a shorter plant height ascompared to a wild-type control plant. In an aspect, a corn plantprovided herein can comprise a recombinant polynucleotide capable ofsuppressing expression of one or more GA20 oxidase and/or GA3 oxidasegene(s) and/or mRNA(s) transcribed therefrom. Alternatively, a cornplant provided herein can comprise one or more mutation(s) or edit(s) inone or more GA20 oxidase and/or GA3 oxidase gene(s). In an aspect, acorn plant provided herein can comprise a mutation in a GA20 oxidaselocus or gene as compared to a wildtype GA20 oxidase locus or gene. Inan aspect, a corn plant provided herein is homozygous (or biallelic) fora mutation or an edit in one or more GA20 oxidase loci or genes ascompared to a wildtype GA20 oxidase locus or gene. In an aspect, a cornplant provided herein is homozygous (or biallelic) for a mutation or anedit in a GA20 oxidase_3 gene as compared to a wildtype GA20 oxidase_3gene. In an aspect, a corn plant provided herein is homozygous (orbiallelic) for a mutation or an edit in a GA20 oxidase_5 gene ascompared to a wildtype GA20 oxidase_5 gene. In another aspect, a cornplant provided herein is heterozygous for a mutation or an edit in oneor more GA20 oxidase loci or genes as compared to a wildtype GA20oxidase locus or gene. In an aspect, a corn plant provided herein isheterozygous for a mutation or an edit in a GA20 oxidase_3 gene ascompared to a wildtype GA20 oxidase_3 gene. In an aspect, a corn plantprovided herein is heterozygous for a mutation or an edit in a GA20oxidase_5 gene as compared to a wildtype GA20 oxidase_5 gene. In anotheraspect, a corn plant provided herein can comprise a mutation in a GA3oxidase locus or gene as compared to a wildtype GA3 oxidase locus orgene. In an aspect, a corn plant provided herein is homozygous (orbiallelic) for a mutation or an edit in one or more GA3 oxidase loci orgenes as compared to a wildtype GA3 oxidase locus or gene. In anotheraspect, a corn plant provided herein is heterozygous for a mutation oran edit in a one or more GA3 oxidase loci or genes as compared to awildtype GA3 oxidase locus or gene. In another aspect, a corn plantprovided herein can comprise a heterologous polynucleotide capable ofsuppressing expression of a GA20 oxidase gene or an mRNA transcribedtherefrom. Additional details about altering the expression of GA20and/or GA3 oxidase gene(s) through suppression, mutation, or editing ofthose gene(s) can be found in PCT Application No. PCT/US2017/047405, theentire contents and disclosure of which is incorporated herein byreference.

In an aspect, a modified corn plant, or plant part thereof, ishomozygous for a mutant allele at an endogenous GA oxidase locus. In anaspect, a modified corn plant, or plant part thereof, is biallelic for afirst mutant allele and a second mutant allele at an endogenous GAoxidase locus. In an aspect, a modified corn plant, or plant partthereof, is heterozygous for a mutant allele at an endogenous GA oxidaselocus. In an aspect, a modified corn plant, or plant part thereof, ishomozygous for a mutant allele at an endogenous GA20 oxidase locus. Inan aspect, a modified corn plant, or plant part thereof, is biallelicfor a first mutant allele and a second mutant allele at an endogenousGA20 oxidase locus. In an aspect, a modified corn plant, or plant partthereof, is heterozygous for a mutant allele at an endogenous GA20oxidase locus. In an aspect, a modified corn plant, or plant partthereof, is homozygous for a mutant allele at an endogenous GA20oxidase_5 locus. In an aspect, a modified corn plant, or plant partthereof, is biallelic for a first mutant allele and a second mutantallele at an endogenous GA20 oxidase_5 locus. In an aspect, a modifiedcorn plant, or plant part thereof, is heterozygous for a mutant alleleat an endogenous GA20 oxidase_5 locus. In an aspect, a modified cornplant, or plant part thereof, is homozygous for a mutant allele at anendogenous GA20 oxidase_3 locus. In an aspect, a modified corn plant, orplant part thereof, is biallelic for a first mutant allele and a secondmutant allele at an endogenous GA20 oxidase_3 locus. In an aspect, amodified corn plant, or plant part thereof, is heterozygous for a mutantallele at an endogenous GA20 oxidase_3 locus. In an aspect, a modifiedcorn plant, or plant part thereof, is homozygous for a mutant allele atan endogenous GA3 oxidase locus. In an aspect, a modified corn plant, orplant part thereof, is biallelic for a first mutant allele and a secondmutant allele at an endogenous GA3 oxidase locus. In an aspect, amodified corn plant, or plant part thereof, is heterozygous for a mutantallele at an endogenous GA3 oxidase locus.

By targeting a subset of one or more endogenous GA3 or GA20 oxidasegenes for suppression within a plant, a more pervasive pattern ofexpression (e.g., with a constitutive promoter) can be used to producesemi-dwarf plants without significant reproductive off-types and/orother undesirable traits in the plant, even with expression of thesuppression construct in reproductive tissue(s). Indeed, suppressionelements and constructs are provided herein that selectively target theGA20 oxidase_3 and/or GA20 oxidase_5 genes for suppression, which can beoperably linked to a vascular, leaf and/or constitutive promoter.

As introduced above, instead of suppressing one or more GA oxidasegene(s), active GA levels can also be reduced in a corn plant bymutation or editing of one or more GA20 and/or GA3 oxidase gene(s).

Corn has a family of at least nine GA20 oxidase genes that includes GA20oxidase_1, GA20 oxidase_2, GA20 oxidase_3, GA20 oxidase_4, GA20oxidase_5, GA20 oxidase_6, GA20 oxidase_7, GA20 oxidase_8, and GA20oxidase_9. However, there are only two GA3 oxidases in corn, GA3oxidase_1 and GA3 oxidase_2. The DNA and protein sequences by SEQ ID NOsfor each of these GA20 oxidase genes are provided in Table 1, and theDNA and protein sequences by SEQ ID NOs for each of these GA3 oxidasegenes are provided in Table 2.

In an aspect, a corn plant provided herein is homozygous (or biallelic)for a mutation or an edit in a GA20 oxidase_5 locus or gene as comparedto a wildtype GA20 oxidase_5 locus or gene and homozygous (or biallelic)for a mutation or an edit in a GA20 oxidase_3 locus or gene as comparedto a wildtype GA20 oxidase_3 locus or gene. In an aspect, a corn plantprovided herein is homozygous (or biallelic) for a mutation or an editin a GA20 oxidase_5 locus or gene as compared to a wildtype GA20oxidase_5 locus or gene and heterozygous for a mutation or an edit in aGA20 oxidase_3 locus or gene as compared to a wildtype GA20 oxidase_3locus or gene. In an aspect, a corn plant provided herein isheterozygous for a mutation or an edit in a GA20 oxidase_5 locus or geneas compared to a wildtype GA20 oxidase_5 locus or gene and homozygous(or biallelic) for a mutation or an edit in a GA20 oxidase_3 locus orgene as compared to a wildtype GA20 oxidase_3 locus or gene. See, e.g.,U.S. Provisional Patent Application Nos. 62/631,412; 62/631,416; and62/710,302; the contents and disclosures of which are incorporatedherein by reference in their entireties.

TABLE 1 DNA and protein sequences by sequence identifier for GA20oxidase genes in corn. GA20 Coding Sequence oxidase Gene cDNA (CDS)Protein GA20 oxidase_1 SEQ ID NO: 1 SEQ ID NO: 2 SEQ ID NO: 3 GA20oxidase_2 SEQ ID NO: 4 SEQ ID NO: 5 SEQ ID NO: 6 GA20 oxidase_3 SEQ IDNO: 7 SEQ ID NO: 8 SEQ ID NO: 9 GA20 oxidase_4 SEQ ID NO: 10 SEQ ID NO:11 SEQ ID NO: 12 GA20 oxidase_5 SEQ ID NO: 13 SEQ ID NO: 14 SEQ ID NO:15 GA20 oxidase_6 SEQ ID NO: 16 SEQ ID NO: 17 SEQ ID NO: 18 GA20oxidase_7 SEQ ID NO: 19 SEQ ID NO: 20 SEQ ID NO: 21 GA20 oxidase_8 SEQID NO: 22 SEQ ID NO: 23 SEQ ID NO: 24 GA20 oxidase_9 SEQ ID NO: 25 SEQID NO: 26 SEQ ID NO: 27

TABLE 2 DNA and protein sequences by sequence identifier for GA3 oxidasegenes in corn. Coding GA3 oxidase Sequence Gene cDNA (CDS) Protein GA3oxidase_1 SEQ ID NO: 28 SEQ ID NO: 29 SEQ ID NO: 30 GA3 oxidase_2 SEQ IDNO: 31 SEQ ID NO: 32 SEQ ID NO: 33

The genomic DNA sequence of GA20 oxidase_3 is provided in SEQ ID NO: 34,and the genomic DNA sequence of GA20 oxidase_5 is provided in SEQ ID NO:35. For the GA20 oxidase_3 gene, SEQ ID NO: 34 provides 3000 nucleotidesupstream of the GA20 oxidase_3 5′-UTR; nucleotides 3001-3096 correspondto the 5′-UTR; nucleotides 3097-3665 correspond to the first exon;nucleotides 3666-3775 correspond to the first intron; nucleotides3776-4097 correspond to the second exon; nucleotides 4098-5314correspond to the second intron; nucleotides 5315-5584 correspond to thethird exon; and nucleotides 5585-5800 correspond to the 3′-UTR. SEQ IDNO: 34 also provides 3000 nucleotides downstream of the end of the3′-UTR (nucleotides 5801-8800).

For the GA20 oxidase_5 gene, SEQ ID NO: 35 provides 3000 nucleotidesupstream of the GA20 oxidase_5 start codon (nucleotides 1-3000);nucleotides 3001-3791 correspond to the first exon; nucleotides3792-3906 correspond to the first intron; nucleotides 3907-4475correspond to the second exon; nucleotides 4476-5197 correspond to thesecond intron; nucleotides 5198-5473 correspond to the third exon; andnucleotides 5474-5859 correspond to the 3′-UTR. SEQ ID NO: 35 alsoprovides 3000 nucleotides downstream of the end of the 3′-UTR(nucleotides 5860-8859).

The genomic DNA sequence of GA3 oxidase_1 is provided in SEQ ID NO: 36,and the genomic DNA sequence of GA3 oxidase_2 is provided in SEQ ID NO:37. For the GA3 oxidase_1 gene, nucleotides 1-29 of SEQ ID NO: 36correspond to the 5′-UTR; nucleotides 30-514 of SEQ ID NO: 36 correspondto the first exon; nucleotides 515-879 of SEQ ID NO: 36 correspond tothe first intron; nucleotides 880-1038 of SEQ ID NO: 36 correspond tothe second exon; nucleotides 1039-1158 of SEQ ID NO: 36 correspond tothe second intron; nucleotides 1159-1663 of SEQ ID NO: 36 correspond tothe third exon; and nucleotides 1664-1788 of SEQ ID NO: 36 correspond tothe 3′-UTR. For the GA3 oxidase_2 gene, nucleotides 1-38 of SEQ ID NO:37 correspond to the 5-UTR; nucleotides 39-532 of SEQ ID NO: 37correspond to the first exon; nucleotides 533-692 of SEQ ID NO: 37correspond to the first intron; nucleotides 693-851 of SEQ ID NO: 37correspond to the second exon; nucleotides 852-982 of SEQ ID NO: 37correspond to the second intron; nucleotides 983-1445 of SEQ ID NO: 37correspond to the third exon; and nucleotides 1446-1698 of SEQ ID NO: 37correspond to the 3′-UTR.

In addition to phenotypic observations with targeting the GA20 oxidase_3and/or GA20 oxidase_5 gene(s), or the GA3 oxidase_1 and/or GA3 oxidase_2gene(s), for suppression, a semi-dwarf phenotype is also observed withsuppression of the GA20 oxidase_4 gene. The genomic DNA sequence of GA20oxidase_4 is provided in SEQ ID NO: 38. For the GA oxidase_4 gene, SEQID NO: 38 provides nucleotides 1-1416 upstream of the 5′-UTR;nucleotides 1417-1543 of SEQ ID NO: 38 correspond to the 5′-UTR;nucleotides 1544-1995 of SEQ ID NO: 38 correspond to the first exon;nucleotides 1996-2083 of SEQ ID NO: 38 correspond to the first intron;nucleotides 2084-2411 of SEQ ID NO: 38 correspond to the second exon;nucleotides 2412-2516 of SEQ ID NO: 38 correspond to the second intron;nucleotides 2517-2852 of SEQ ID NO: 38 correspond to the third exon;nucleotides 2853-3066 of SEQ ID NO: 38 correspond to the 3′-UTR; andnucleotides 3067-4465 of SEQ ID NO: 38 corresponds to genomic sequencedownstream of to the 3′-UTR.

In an aspect, the present disclosure provides a corn plant or pluralityof corn plants each comprising a recombinant DNA construct orpolynucleotide sequence comprising a transcribable DNA sequence encodinga non-coding RNA molecule, wherein the non-coding RNA molecule comprisesa sequence that is at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% complementary to at least 15, at least 16, at least 17,at least 18, at least 19, at least 20, at least 21, at least 22, atleast 23, at least 24, at least 25, at least 26, or at least 27consecutive nucleotides of a mRNA molecule encoding an endogenous GAoxidase protein in a corn plant or corn cell, the endogenous GA oxidaseprotein being at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to SEQ ID NO: 9, 12, 15, 30, and/or 33, and wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter, which can be heterologous with respect to the transcribableDNA sequence and/or the corn plant.

Recombinant DNA constructs and transgenic corn plants are providedherein comprising a GA20 or GA3 oxidase suppression element or sequenceoperably linked to a plant expressible promoter, which can be aconstitutive or tissue-specific or tissue-preferred promoter. Such atissue-specific or tissue-preferred promoter can drive expression of itsassociated GA oxidase suppression element or sequence in one or moreactive GA-producing tissue(s) of the plant to suppress or reduce thelevel of active GAs produced in those tissue(s). Such a tissue-specificor tissue-preferred promoter can drive expression of its associated GAoxidase suppression construct or transgene during one or more vegetativestage(s) of development. Such a tissue-specific or tissue-preferredpromoter can also have little or no expression in one or more cell(s) ortissue(s) of the developing female organ or ear of the plant to avoidthe possibility of off-types in those reproductive tissues.

As used herein, a “plant-expressible promoter” refers to a promoter thatdrives, causes, or initiates expression of a transcribable DNA sequenceor transgene operably linked to such promoter in one or more plant cellsor tissues, such as one or more cells or tissues of a corn plant. In anaspect, a plant-expressible promoter is a constitutive promoter. Inanother aspect, a plant-expressible promoter is a vascular promoter. Asused herein, a “vascular promoter” refers to a plant-expressiblepromoter that drives, causes or initiates expression of a transcribableDNA sequence or transgene operably linked to such promoter in one ormore vascular tissue(s) of the plant, even if the promoter is alsoexpressed in other non-vascular plant cell(s) or tissue(s). Suchvascular tissue(s) can comprise one or more of the phloem, vascularparenchymal, and/or bundle sheath cell(s) or tissue(s) of the plant. A“vascular promoter” is distinguished from a constitutive promoter inthat it has a regulated and relatively more limited pattern ofexpression that includes one or more vascular tissue(s) of the plant. Avascular promoter includes both vascular-specific promoters andvascular-preferred promoters. In another aspect, a plant-expressiblepromoter is a leaf promoter. As used herein, a “leaf promoter” refers toa plant-expressible promoter that drives, causes or initiates expressionof a transcribable DNA sequence or transgene operably linked to suchpromoter in one or more leaf tissue(s) of the plant, even if thepromoter is also expressed in other non-leaf plant cell(s) or tissue(s).A leaf promoter includes both leaf-specific promoters and leaf-preferredpromoters. A “leaf promoter” is distinguished from a vascular promoterin that it is expressed more predominantly or exclusively in leaftissue(s) of the plant relative to other plant tissues, whereas avascular promoter is expressed in vascular tissue(s) more generallyincluding vascular tissue(s) outside of the leaf, such as the vasculartissue(s) of the stem, or stem and leaves, of the plant.

Promoters that drive enhanced expression in certain tissues of the plantrelative to other plant tissues are referred to as “tissue-enhanced” or“tissue-preferred” promoters. Thus, a “tissue-preferred” promoter causesrelatively higher or preferential or predominant expression in aspecific tissue(s) of the plant, but with lower levels of expression inother tissue(s) of the plant. Promoters that express within a specifictissue(s) of the plant, with little or no expression in other planttissues, are referred to as “tissue-specific” promoters.

A non-limiting exemplary plant-expressible promoter is the RTBVpromoter. In an aspect, a plant-expressible promoter is an RTBVpromoter. In another aspect, a plant expressible promoter comprises aDNA sequence that is at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5% or 100% identical to one or more of SEQ ID NO: 39, SEQ ID NO: 40,or a functional portion thereof.

Non-limiting exemplary vascular promoters include a sucrose synthasepromoter, a sucrose transporter promoter, a Sh1 promoter, Commelinayellow mottle virus (CoYMV) promoter, a wheat dwarf geminivirus (WDV)large intergenic region (LIR) promoter, a maize streak geminivirus (MSV)coat protein (CP) promoter, a rice yellow stripe 1 (YS1)-like promoter,and a rice yellow stripe 2 (OsYSL2) promoter. In an aspect, a vascularpromoter is selected from the group consisting of a sucrose synthasepromoter, a sucrose transporter promoter, a Sh1 promoter, Commelinayellow mottle virus (CoYMV) promoter, a wheat dwarf geminivirus (WDV)large intergenic region (LIR) promoter, a maize streak geminivirus (MSV)coat protein (CP) promoter, a rice yellow stripe 1 (YS1)-like promoter,a rice yellow stripe 2 (OsYSL2) promoter, and functional portionsthereof. In an aspect, a vascular promoter comprises a DNA sequence thatis at least 80%, at least 85%, at least 90%, at least 95%, at least 96%,at least 97%, at least 98%, at least 99%, at least 99.5% or 100%identical to one or more of SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43,SEQ ID NO: 44, SEQ ID NO: 45, or a functional portion thereof.

Non-limiting exemplary leaf promoters include a RuBisCO promoter, a PPDKpromoter, a FDA promoter, a Nadh-Gogat promoter, a chlorophyll a/bbinding protein gene promoter, a phosphoenolpyruvate carboxylase (PEPC)promoter, and a Myb gene promoter. In an aspect, a leaf promoter isselected from the group consisting of a RuBisCO promoter, a PPDKpromoter, a FDA promoter, a Nadh-Gogat promoter, a chlorophyll a/bbinding protein gene promoter, a phosphoenolpyruvate carboxylase (PEPC)promoter, a Myb gene promoter, and functional portions thereof. In anaspect, a leaf promoter comprises a DNA sequence that is at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5% or 100% identical to one or moreof SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, or a functional portionthereof.

Non-limiting exemplary constitutive promoters include an actin promoter,a CaMV 35S or 19S promoter, a plant ubiquitin promoter, a plant Gos2promoter, a FMV promoter, a CMV promoter, a MMV promoter, a PCLSVpromoter, an Emu promoter, a tubulin promoter, a nopaline synthasepromoter, an octopine synthase promoter, a mannopine synthase promoter,and a maize alcohol dehydrogenase. In an aspect, a constitutive promoteris selected from the group consisting of an actin promoter, a CaMV 35Sor 19S promoter, a plant ubiquitin promoter, a plant Gos2 promoter, aFMV promoter, a CMV promoter, a MMV promoter, a PCLSV promoter, an Emupromoter, a tubulin promoter, a nopaline synthase promoter, an octopinesynthase promoter, a mannopine synthase promoter, a maize alcoholdehydrogenase, or functional portions thereof. In an aspect, aconstitutive promoter comprises a DNA sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5% or 100% identical to one or moreof SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ IDNO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, or afunctional portion thereof.

In another aspect, the present disclosure provides a corn plant orplurality of corn plants each comprising a recombinant DNA construct orpolynucleotide sequence comprising a transcribable DNA sequence encodinga non-coding RNA molecule, wherein the non-coding RNA molecule comprisesa sequence that is at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% complementary to at least 15, at least 16, at least 17,at least 18, at least 19, at least 20, at least 21, at least 22, atleast 23, at least 24, at least 25, at least 26, or at least 27consecutive nucleotides of a mRNA molecule encoding an endogenous GAoxidase gene having at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 9, 12, 15, 30, and/or 33, andwherein the transcribable DNA sequence is operably linked to aplant-expressible promoter, which can be heterologous with respect tothe transcribable DNA sequence and/or the corn plant.

As provided above, a corn plant or plant part can comprise a firstexpression cassette comprising a first sequence encoding a non-codingRNA molecule that targets one or more GA20 or GA3 oxidase gene(s) forsuppression. In an aspect, the non-coding RNA molecule can target one ormore GA20 oxidase gene(s) for suppression, such as a GA20 oxidase_3gene, a GA20 oxidase_4 gene, a GA20 oxidase_5 gene, or any combinationthereof. According to some embodiments, the first expression cassettecomprises a first transcribable DNA sequence encoding a non-coding RNAtargeting a GA20 oxidase_3 gene for suppression. According to otherembodiments, the first expression cassette comprises a firsttranscribable DNA sequence encoding a non-coding RNA targeting a GA20oxidase_5 gene for suppression. According to yet further embodiments,the first expression cassette comprises a first transcribable DNAsequence encoding a non-coding RNA that targets both the GA20 oxidase_3gene and the GA20 oxidase_5 gene for suppression. In addition totargeting a mature mRNA sequence (including either or both of theuntranslated or exonic sequences), a non-coding RNA molecule can alsotarget the intronic sequences of a GA20 oxidase gene or transcript.

For suppression of a GA20 oxidase_3 gene, a first transcribable DNAsequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 7, SEQ ID NO: 8, and SEQ ID NO: 34.

For suppression of a GA20 oxidase_4 gene, a first transcribable DNAsequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 10, SEQ ID NO: 11, and SEQ ID NO: 38.

For suppression of a GA20 oxidase_5 gene, a first transcribable DNAsequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 13, SEQ ID NO: 14, and SEQ ID NO: 35.

For suppression of a GA20 oxidase_3 gene and a GA20 oxidase_5 gene, atranscribable DNA sequence comprises a sequence that is at least 80%, atleast 81%, at least 82%, at least 83%, at least 84%, at least 85%, atleast 86%, at least 87%, at least 88%, at least 89%, at least 90%, atleast 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical or complementary to at least 15, at least 16, at least17, at least 18, at least 19, at least 20, at least 21, at least 22, atleast 23, at least 24, at least 25, at least 26, at least 27, at least28, at least 29, at least 30, at least 31, at least 32, at least 33, atleast 34, at least 35, at least 36, at least 37, at least 38, at least39, at least 40, at least 41, at least 42, at least 43, at least 44, atleast 45, at least 46, at least 47, at least 48, at least 49, at least50, at least 51, at least 52, at least 53, at least 54, at least 55, atleast 56, at least 57, at least 58, at least 59, or at least 60consecutive nucleotides of a sequence selected from the group consistingof SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 34; and the transcribableDNA sequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 13, SEQ ID NO: 14, and SEQ ID NO: 35.

In another aspect, a first expression cassette comprises a firsttranscribable DNA sequence encoding a non-coding RNA targeting a GA3oxidase gene(s) for suppression in corn, such as a GA3 oxidase_1 gene ora GA3 oxidase_2 gene. In another aspect, a first transcribable DNAsequence encoding a non-coding RNA targets both the GA3 oxidase_1 geneand the GA3 oxidase_2 gene for suppression. In addition to targeting amature mRNA sequence (including either or both of the untranslated orexonic sequences), a non-coding RNA molecule can also target theintronic sequences of a GA3 oxidase gene or transcript.

For suppression of a GA3 oxidase_1 gene, a first transcribable DNAsequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 28, SEQ ID NO: 29, and SEQ ID NO: 36.

For suppression of a GA3 oxidase_2 gene, a first transcribable DNAsequence comprises a sequence that is at least 80%, at least 81%, atleast 82%, at least 83%, at least 84%, at least 85%, at least 86%, atleast 87%, at least 88%, at least 89%, at least 90%, at least 91%, atleast 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalor complementary to at least 15, at least 16, at least 17, at least 18,at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 31, at least 32, at least 33, at least 34, atleast 35, at least 36, at least 37, at least 38, at least 39, at least40, at least 41, at least 42, at least 43, at least 44, at least 45, atleast 46, at least 47, at least 48, at least 49, at least 50, at least51, at least 52, at least 53, at least 54, at least 55, at least 56, atleast 57, at least 58, at least 59, or at least 60 consecutivenucleotides of a sequence selected from the group consisting of SEQ IDNO: 31, SEQ ID NO: 32, and SEQ ID NO: 37.

For suppression of a GA3 oxidase_1 gene and a GA3 oxidase_2 gene, atranscribable DNA sequence comprises a sequence that is at least 80%, atleast 81%, at least 82%, at least 83%, at least 84%, at least 85%, atleast 86%, at least 87%, at least 88%, at least 89%, at least 90%, atleast 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical or complementary to at least 15, at least 16, at least17, at least 18, at least 19, at least 20, at least 21, at least 22, atleast 23, at least 24, at least 25, at least 26, at least 27, at least28, at least 29, at least 30, at least 31, at least 32, at least 33, atleast 34, at least 35, at least 36, at least 37, at least 38, at least39, at least 40, at least 41, at least 42, at least 43, at least 44, atleast 45, at least 46, at least 47, at least 48, at least 49, at least50, at least 51, at least 52, at least 53, at least 54, at least 55, atleast 56, at least 57, at least 58, at least 59, or at least 60consecutive nucleotides of a sequence selected from the group consistingof SEQ ID NO: 28, SEQ ID NO: 29, and SEQ ID NO: 36; and thetranscribable DNA sequence comprises a sequence that is at least 80%, atleast 81%, at least 82%, at least 83%, at least 84%, at least 85%, atleast 86%, at least 87%, at least 88%, at least 89%, at least 90%, atleast 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical or complementary to at least 15, at least 16, at least17, at least 18, at least 19, at least 20, at least 21, at least 22, atleast 23, at least 24, at least 25, at least 26, at least 27, at least28, at least 29, at least 30, at least 31, at least 32, at least 33, atleast 34, at least 35, at least 36, at least 37, at least 38, at least39, at least 40, at least 41, at least 42, at least 43, at least 44, atleast 45, at least 46, at least 47, at least 48, at least 49, at least50, at least 51, at least 52, at least 53, at least 54, at least 55, atleast 56, at least 57, at least 58, at least 59, or at least 60consecutive nucleotides of a sequence selected from the group consistingof SEQ ID NO: 31, SEQ ID NO: 32 and SEQ ID NO: 37.

In an aspect, a mutant allele of an endogenous GA20 oxidase_3 locuscomprises a DNA segment inserted into the endogenous GA20 oxidase_3locus, where the DNA segment encodes an antisense RNA sequence that isat least 70%, at least 80%, at least 90%, at least 91%, at least 92%, atleast 93%, at least 94%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, or 100% complementary to at least 20, at least30, at least 40, at least 50, at least 100, at least 200, at least 300,at least 400, at least 500, at least 600, at least 700, at least 800, atleast 900, or at least 1000 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and where the mutant allele of theendogenous GA20 oxidase_3 locus produces an RNA transcript comprisingthe antisense RNA sequence.

In an aspect, a mutant allele of an endogenous GA20 oxidase_5 locuscomprises a DNA segment inserted into the endogenous GA20 oxidase_5locus, where the DNA segment encodes an antisense RNA sequence that isat least 70%, at least 80%, at least 90%, at least 91%, at least 92%, atleast 93%, at least 94%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, or 100% complementary to at least 20, at least30, at least 40, at least 50, at least 100, at least 200, at least 300,at least 400, at least 500, at least 600, at least 700, at least 800, atleast 900, or at least 1000 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and where the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence.

In an aspect, a mutant allele of the endogenous GA20 oxidase_3 locussuppresses the expression of a wild-type allele of the endogenous GA20oxidase_3 locus, a wild-type allele of the endogenous GA20 oxidase_5locus, or both. In an aspect, a mutant allele of the endogenous GA20oxidase_5 locus suppresses the expression of a wild-type allele of theendogenous GA20 oxidase_3 locus, a wild-type allele of the endogenousGA20 oxidase_5 locus, or both. In an aspect, a mutant allele comprises adeletion of at least one portion of an endogenous GA20 oxidase_3 locus.

Without being bound by any scientific theory, if a genomic regionbetween the neighboring Zm.GA20ox5 and Zm.SAMT genes (including possiblyall or part of those genes) is deleted, then the endogenous Zm.SAMT genepromoter can drive expression of an antisense RNA transcript through allor part of the Zm.GA20ox5 gene that can hybridize to a separate RNAtranscript expressed from one or both of the copies or alleles of theZm.GA20ox5 and/or Zm.GA20ox3 gene(s). Thus, a mutant allele having adeletion between the Zm.GA20ox5 and Zm.SAMT genes can behave as adominant negative mutation or allele by causing suppression or silencingof one or both (wild-type and/or mutant) copies or alleles of theendogenous Zm.GA20ox5 gene, in addition to possible further suppressionor silencing of one or both copies or alleles of the endogenousZm.GA20ox3 gene.

According to aspects of the present disclosure, a mutant or editedallele of the endogenous GA20 oxidase_5 (GA20ox5) gene or locus isprovided comprising a deletion between the neighboring Zm.GA20ox5 andZm.SAMT genes, such that an antisense RNA molecule that is complementaryto all or part of the coding sequence of the GA20ox5 gene may betranscribed under the control of the endogenous Zm.SAMT gene promoter.It is contemplated that the antisense RNA molecule transcribed from themutant or edited allele of the endogenous GA20 oxidase_5 gene or locusmay affect the expression level(s) of the GA20 oxidase_5 and/orendogenous GA20 oxidase_3 gene(s) through different mechanisms, such asnonsense mediated decay, non-stop decay, no-go decay, DNA or histonemethylation or other epigenetic changes, inhibition or decreasedefficiency of transcription and/or translation, ribosomal interference,interference with mRNA processing or splicing, and/or ubiquitin-mediatedprotein degradation via the proteasome. See, e.g., Nickless, A. et al.,“Control of gene expression through the nonsense-mediated RNA decaypathway”, Cell Biosci 7:26 (2017); Karamyshev, A. et al., “Lost inTranslation: Ribosome-Associated mRNA and Protein Quality Controls”,Frontiers in Genetics 9:431 (2018); Inada, T., “Quality controls inducedby aberrant translation”, Nucleic Acids Res 48:3 (2020); andSzadeczky-Kardoss, I. et al., “The nonstop decay and the RNA silencingsystems operate cooperatively in plants”, Nucleic Acids Res 46:9 (2018),the entire contents and disclosures of which are incorporated herein byreference. Each of these different mechanisms may act alternatively orin addition to RNA interference (RNAi), transcriptional gene silencing(PGS) and/or post transcriptional gene silencing (PTGS) mechanisms. See,e.g., Wilson, R. C. et al., “Molecular Mechanisms of RNA Interference”,Annu Rev Biophysics 42:217-39 (2013); and Guo, Q. et al., “RNA Silencingin Plants: Mechanism, Technologies and Applications in HorticultureCrops”, Current Genomics 17:476-489 (2016), the entire contents anddisclosures of which is incorporated herein by reference. Some of theabove mechanisms may reduce expression of the edited allele itself,while others may also reduce the expression of other copy/-ies orallele(s) of the endogenous GA20 oxidase_5 and/or GA20 oxidase_3locus/loci or gene(s). Indeed, it is envisioned that the editedendogenous GA20 oxidase_5 locus, gene or allele may not only reduce oreliminate its own expression and/or activity level, but may also have adominant or semi-dominant effect(s) on the other copy/-ies or allele(s)of the endogenous GA20 oxidase_5 and/or GA20 oxidase_3 locus/loci orgene(s). Such dominant or semi-dominant effect(s) on the GA20 oxidase_5and/or GA20 oxidase_3 gene(s) may operate through non-canonicalsuppression mechanisms that do not involve RNAi and/or formation oftargeted small RNAs at a significant or detectable level.

As used herein, an “intergenic region” or “intergenic sequence” refersto a genomic region or a polynucleotide sequence located in betweentranscribed regions of two neighboring genes. For example, theendogenous Zm.GA20ox5 gene and its neighboring gene in the corn or maizegenome, the s-adenosyl methyl transferase (SAMT) or Zm.SAMT gene,contains an intergenic region between the 3′ UTR of the Zm. GA20ox5 geneand the 3′ UTR of the Zm.SAMT gene.

In the corn genome, the Zm.GA20ox5 gene located next to the Zm.SAMTgene. These two genes are separated by an intergenic region of about 550bp, with the Zm.SAMT gene positioned downstream and oriented in theopposite orientation relative to the Zm.GA20ox5 gene. A referencegenomic sequence of the region encompassing the Zm.GA20ox5 and Zm.SAMTgenes is provided in SEQ ID NOs. 226 and 227. SEQ ID NO. 226 representsthe sequence of the sense strand of the Zm.GA20ox5 gene encompassingboth Zm.GA20ox5 and Zm.SAMT genes. SEQ ID NO: 226 partially overlapswith SEQ ID NO: 222 and has a shorter Zm.GA20ox5 upstream sequence and alonger Zm.GA20ox5 downstream sequence compared to the SEQ ID NO: 222.SEQ ID NO. 227 represents the sequence of the sense strand of theZm.SAMT gene (i.e., the antisense strand of the Zm.GA20ox5 gene)encompassing both Zm.GA20ox5 and Zm.SAMT genes.

In an aspect, a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the transcription termination sequenceof the endogenous Zm.SAMT gene, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene.

In an aspect, a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of the intergenic region between theendogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutantallele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene.

In an aspect, a mutant allele of the endogenous GA20 oxidase_5 locus,wherein the mutant allele comprises a genome modification comprising adeletion of at least a portion of one or more of the following: 5′ UTR,1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′UTR, and any portion thereof, and the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any portionthereof, of the endogenous Zm.SAMT gene.

In an aspect, a mutant allele comprises a genome modification whichresults in the transcription of an antisense strand of at least an exon,an intron, or an untranslated region (UTR) of an endogenous GA20oxidase_5 gene, or any portion thereof.

In an aspect, a mutant allele comprises the Zm.SAMT gene promoter, or afunctional part thereof, operably linked to at least one transcribableantisense sequence of at least an exon, intron or untranslated region(UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof.

In an aspect, a mutant allele comprises a sequence selected from thegroup consisting of SEQ ID NOs: 304-322.

In an aspect, a mutant allele comprises a first sequence and a secondsequence; wherein the first sequence comprises one or more of the 5′UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)exon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3rd intron, 4^(th)exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.SAMT gene; wherein the first sequence and the secondsequence are contiguous or separated only by an intervening sequence offewer than 555 nucleotides. In an aspect, a mutant allele comprises agenomic deletion relative to a wild type allele of the endogenous GA20oxidase_5 locus, wherein the genomic deletion is flanked by a firstsequence and a second sequence; wherein the first sequence comprises oneor more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)intron, 3^(rd) exon, 3′ UTR, and any complementary sequence thereof, andany portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;and wherein the second sequence comprises one or more of the 5′ UTR,1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th) intron,6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′UTR, and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.SAMT gene. In an aspect, a mutant allelecomprises a genomic sequence comprising a first sequence and a secondsequence; wherein the first sequence comprises at least 15, at least 20,at least 25, at least 30, at least 35, at least 40, at least 45, atleast 50, at least 100, at least 200, at least 300, at least 400, atleast 500, at least 750, or at least 1000 consecutive nucleotides of oneor more of SEQ ID NOs: 228-235 and 276-283; wherein the second sequencecomprises at least 15, at least 20, at least 25, at least 30, at least35, at least 40, at least 45, at least 50, at least 100, at least 200,at least 300, at least 400, at least 500, at least 750, or at least 1000consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50, at least 100, at least 250,at least 500, or at least 1000 consecutive nucleotides in length, and/orfewer than 9000 consecutive nucleotides in length. In an aspect, a firstsequence comprises one or more of SEQ ID NOs: 228-235 and 276-283, orany portion thereof, and a second sequence comprises one or more of SEQID NOs: 235-276, or any portion thereof. In an aspect, a first sequencecomprises one or more of SEQ ID NOs: 226-235 and 276-283, or any portionthereof, and a second sequence comprises one or more of SEQ ID NOs: 226,227, and 235-276, or any portion thereof. In an aspect, a first sequencecomprises at least 15, at least 20, at least 25, at least 30, at least35, at least 40, at least 45, at least 50, at least 100, at least 200,at least 300, at least 400, at least 500, at least 750, or at least 1000consecutive nucleotides of one or more of SEQ ID NOs: 226-235 and276-283, and a second sequence comprises at least 15, at least 20, atleast 25, at least 30, at least 35, at least 40, at least 45, at least50, at least 100, at least 200, at least 300, at least 400, at least500, at least 750, or at least 1000 consecutive nucleotides of one ormore of SEQ ID NOs: 226, 227, and 235-276.

In an aspect, a genome modification further comprises the deletion of atleast a portion of the transcription termination sequence of anendogenous GA20 oxidase_5 gene. In an aspect, a genome modificationcomprises a deletion of one or both of the transcription terminationsequences of an endogenous GA20 oxidase_5 gene and an endogenous Zm.SAMTgene. In an aspect, a genome modification comprises a deletion of atleast 10, at least 15, at least 20, at least 25, at least 30, at least35, at least 40, at least 45, at least 50, at least 100, at least 200,at least 300, at least 400, at least 500, at least 750, or at least 1000consecutive nucleotides of the intergenic region between the endogenousGA20 oxidase_5 and SAMT genes. In an aspect, a genome modificationcomprises a deletion of the entire intergenic region between theendogenous GA20 oxidase_5 and SAMT genes. In an aspect, a genomemodification comprises a deletion of one or more sequence elementsselected from the group consisting of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portionof the foregoing, of an endogenous GA20 oxidase_5 gene. In an aspect, agenome modification comprises a deletion of one or more sequenceelements selected from the group consisting of the 5′ UTR, 1^(st) exon,1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron,4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon,6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and anyportion of the foregoing, of the endogenous Zm.SAMT locus. In an aspect,a genome modification results in the production of an RNA moleculecomprising an antisense sequence from a genomic segment of selected fromthe group consisting of an exon, a portion of an exon, an intron, aportion of an intron, a 5′ or 3′ untranslated region (UTR), a portion ofan UTR, and any combination of the foregoing, of the endogenous GA20oxidase_5 locus. In an aspect, a genome modification comprises two ormore, three or more, four or more, five or more, or six or morenon-contiguous deletions.

In an aspect, a genomic deletion comprises a deletion of the intergenicregion between the endogenous Zm.GA20 oxidase_5 and Zm.SAMT genes. In anaspect, a genomic deletion has a length of at least 50, at least 100, atleast 250, at least 500, at least 750, at least 1000, at least 2500, orat least 5000 nucleotides. In an aspect, a genomic deletion has a lengthof at most 7500, at most 7000, at most 6000, at most 5000, at most 4000,at most 3000, at most 2500, at most 2000, at most 1000, or at most 500nucleotides. In an aspect, a genomic deletion corresponds to a deletionof one or more genomic regions comprising a sequence selected from thegroup consisting of SEQ ID NOs: 228-283. In an aspect a genome deletionresults in the production of an RNA transcript comprising an antisensesequence from a genomic segment of the endogenous GA20 oxidase_5 locusselected from the group consisting of an exon, portion of an exon, anintron, portion of an intron, an untranslated region (UTR), portion ofan UTR, and any combination of the foregoing.

In an aspect, a mutant allele comprises the endogenous Zm.SAMT genepromoter, or a portion thereof, operably linked to a transcribable DNAsequence encoding a RNA molecule that causes suppression of one or bothof the endogenous GA20 oxidase_3 gene and the endogenous GA20 oxidase_5gene. In an aspect, a mutant allele comprises the endogenous Zm.SAMTgene promoter, or a portion thereof, operably linked to a transcribableDNA sequence encoding a RNA molecule comprising an antisense sequencethat is at least 80%, at least 85%, at least 90%, at least 91%, at least92%, at least 93%, at least 94%, at least 95%, at least 96%, at least97%, at least 98%, at least 99%, or 100% complementary to all or part ofthe endogenous GA20 oxidase_3 or GA20 oxidase_5 gene. In an aspect, atranscribable DNA sequence is at least 80%, at least 85%, at least 90%,at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, or 100%complementary to an RNA transcript sequence, or a portion thereof,encoded by an endogenous GA20 oxidase_3 or GA20 oxidase_5 gene. In anaspect, a transcribable DNA sequence is at least 80% complementary to atleast 15, at least 16, at least 17, at least 18, at least 19, at least20, at least 21, at least 22, at least 23, at least 24, at least 25, atleast 26, at least 27, at least 28, at least 29, at least 30, at least40, at least 50, at least 60, at least 70, at least 80, at least 90, atleast 100, or at least 200 consecutive nucleotides of one or more of SEQID NOs: 218-220, 222-224, 226, and 228-255. In an aspect, atranscribable DNA sequence is at least 80% complementary to at least 15,at least 16, at least 17, at least 18, at least 19, at least 20, atleast 21, at least 22, at least 23, at least 24, at least 25, at least26, at least 27, at least 28, at least 29, at least 30, at least 40, atleast 50, at least 60, at least 70, at least 80, at least 90, at least100, or at least 200 consecutive nucleotides of one or more of SEQ IDNOs: 222-224 and 228-235.

In an aspect, a DNA segment comprises a nucleotide sequence originatingfrom the endogenous GA20 oxidase_3 locus. In an aspect, a DNA segmentcorresponds to an inverted genomic fragment of the endogenous GA20oxidase_3 locus. In an aspect, a DNA segment comprises a nucleotidesequence originating from an endogenous GA20 oxidase_5 locus. In anaspect, a DNA segment is inserted near or adjacent to a correspondingendogenous DNA segment of the endogenous GA20 oxidase_3 locus. In anaspect, the sense strand of a DNA segment comprises a sequence at least70%, at least 80%, at least 90%, at least 91%, at least 92%, at least93%, at least 94%, at least 95%, at least 96%, at least 97%, at least98%, at least 99%, or 100% complementary to an exon sequence of theendogenous GA20 oxidase_3 or GA20 oxidase_5 locus. In an aspect, thesense strand of a DNA segment comprises a sequence at least 70%, atleast 80%, at least 90%, at least 91%, at least 92%, at least 93%, atleast 94%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, or 100% complementary to an untranslated region (UTR)sequence of the endogenous GA20 oxidase_3 or GA20 oxidase_5 locus. In anaspect, the sense strand of a DNA segment comprises a sequence at least70%, at least 80%, at least 90%, at least 91%, at least 92%, at least93%, at least 94%, at least 95%, at least 96%, at least 97%, at least98%, at least 99%, or 100% complementary to an exon sequence and anintron sequence of the endogenous GA20 oxidase_3 or GA20 oxidase_5locus, the exon sequence and the intron sequence being contiguous withinthe endogenous locus. In an aspect, a DNA segment comprises a sequencehaving at least at least 70%, at least 80%, at least 90%, at least 91%,at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, or 100% identity to one or moreof SEQ ID NOs: 194, 195, 207, 209, 211, 213, and 217.

In an aspect, a corresponding endogenous sequence of an RNA transcriptis at least 85%, at least 90%, at least 91%, at least 92%, at least 93%,at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, or 100% identical to at least 20, at least 30, at least 40,at least 50, at least 100, at least 200, at least 300, at least 400, atleast 500, at least 600, at least 700, at least 800, at least 900, or atleast 1000 consecutive nucleotides of one or more of SEQ ID NOs: 182-184and 186-188. In an aspect, an antisense RNA sequence forms a stem-loopstructure with the corresponding endogenous sequence of the RNAtranscript. In an aspect, an RNA transcript further comprises one ormore sequence elements of the endogenous GA20 oxidase_5 locus selectedfrom the group consisting of 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion thereof.

In an aspect, an RNA transcript sequence comprises a sequence that is atleast 85%, at least 90%, at least 91%, at least 92%, at least 93%, atleast 94%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, or 100% identical to at least 20, at least 30, at least 40,at least 50, at least 100, at least 200, at least 300, at least 400, atleast 500, at least 600, at least 700, at least 800, at least 900, or atleast 1000 consecutive nucleotides of one or more of SEQ ID NOs:218-220, 222-224, 226, and 228-255. In an aspect, an RNA transcriptsequence comprises a sequence that is at least 85%, at least 90%, atleast 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, or 100% identicalto at least 20, at least 30, at least 40, at least 50, at least 100, atleast 200, at least 300, at least 400, at least 500, at least 600, atleast 700, at least 800, at least 900, or at least 1000 consecutivenucleotides of one or more of SEQ ID NOs: 222-224 and 228-235.

In an aspect, an inserted DNA segment is located upstream (e.g. on the5′ side) of a corresponding endogenous DNA segment. In an aspect, aninserted DNA segment is located downstream (e.g. on the 3′ side) of acorresponding endogenous DNA segment. In an aspect, a DNA segmentcomprises a length of at least 10, at least 15, at least 20, at least25, at least 30, at least 40, at least 50, at least 75, at least 100, atleast 200, at least 300, at least 400, at least 500, at least 600, atleast 700, at least 800, or at least 900 nucleotides. In an aspect, aDNA segment comprises a length of at most 2000, at most 1500, at most1000, at most 900, at most 800, at most 700, at most 600, at most 500,at most 400, at most 300, at most 200, at most 100, at most 75, at most50, or at most 25 nucleotides.

In an aspect, an inserted DNA segment and the corresponding endogenousDNA segment of a mutant allele are separated by an intervening DNAsequence. In an aspect, an intervening DNA sequence comprises a lengthof at least 1 nucleotide. In an aspect, an intervening DNA sequencecomprises a length of at least 2, at least 3, at least 4, at least 5, atleast 10, at least 20, at least 30, at least 40, at least 50, at least75, at least 100, at least 200, at least 300, at least 400, at least500, at least 600, at least 700, at least 800, at least 900, at least1000, at least 1500, at least 2000, at least 2500, at least 3000, or atleast 40000 consecutive nucleotides. In an aspect, an intervening DNAsequence comprises at most 5000, at most 4000, at most 3000, at most2000, at most 1500, at most 1000, at most 750, at most 500, at most 250,at most 100, at most 75, at most 50, at most 25, at most 10, or at most5 consecutive nucleotides.

In an aspect, an intervening DNA sequence encodes an intervening RNAsequence between an antisense RNA sequence and a correspondingendogenous sequence of an RNA transcript. In an aspect, an RNAtranscript forms a stem-loop structure with an intervening RNA sequenceforming the loop portion of the stem-loop structure. In an aspect, astem-loop secondary structure comprises a near-perfect-complement stemwith mismatches. In an aspect, a near-perfect-complement stem comprisesfewer than 10, fewer than 9, fewer than 8, fewer than 7, fewer than 6,fewer than 5, fewer than 4, fewer than 3, or fewer than 2 mismatches. Inan aspect, a stem-loop secondary structure comprises a perfectcomplement stem with zero mismatches. In an aspect, an intervening DNAsequence comprises an intron sequence. In an aspect, an intervening DNAsequence does not comprise an intron sequence.

In an aspect, an intervening DNA sequence comprises a native sequence ofthe endogenous GA20 oxidase_3 locus. In an aspect, an intervening DNAsequence comprises an exogenous sequence inserted into the endogenousGA20 oxidase_3 locus. In an aspect, an intervening DNA sequencecomprises a native sequence of the endogenous GA20 oxidase_5 locus. Inan aspect, an intervening DNA sequence comprises an exogenous sequenceinserted into the endogenous GA20 oxidase_5 locus. In an aspect, a DNAsegment is inserted within a region selected from the group consistingof 5′ untranslated region (UTR), 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon and 3′ UTR of the endogenous GA20oxidase_3 locus, and a combination thereof. In an aspect a DNA segmentis inserted at a genomic site recognized by a targeted editing techniqueto create a double-stranded break (DSB). In an aspect, a DNA segmentcomprises a nucleotide sequence originating from an endogenous GA20oxidase_3 locus. In an aspect, a DNA segment corresponds to an invertedgenomic fragment of the endogenous GA20 oxidase_3 locus. In an aspect, aDNA segment comprises a nucleotide sequence originating from theendogenous GA20 oxidase_5 locus. In an aspect, a DNA segment correspondsto an inverted genomic fragment of the endogenous GA20 oxidase_5 locus.In an aspect, a DNA segment is inserted near or adjacent to acorresponding endogenous DNA segment of the endogenous GA20 oxidase_5locus.

In an aspect, this disclosure provides a corn plant where the level ofone or more active GAs in at least one internode tissue of the stem orstalk of the modified corn plant is lower than the same internode tissueof an unmodified control plant.

Any method known in the art for suppression of a target gene can be usedto suppress GA oxidase or brachytic gene(s) according to aspects of thepresent disclosure including expression of antisense RNAs, doublestranded RNAs (dsRNAs) or inverted repeat RNA sequences, or viaco-suppression or RNA interference (RNAi) through expression of smallinterfering RNAs (siRNAs), short hairpin RNAs (shRNAs), trans-actingsiRNAs (ta-siRNAs), or micro RNAs (miRNAs). Collectively, antisenseRNAs, dsRNAs, inverted repeat RNA sequences, siRNAs, shRNAs, ta-siRNAs,and miRNAs are referred to herein as “non-coding RNAs.” Furthermore,sense and/or antisense RNA molecules can be used that target thenon-coding genomic sequences or regions within or near a gene to causesilencing of the gene. Accordingly, any of these methods can be used forthe targeted suppression of an endogenous GA oxidase gene(s) or br2 in atissue-specific or tissue-preferred manner. See, e.g., U.S. PatentApplication Publication Nos. 2009/0070898, 2011/0296555, and2011/0035839, the contents and disclosures of which are incorporatedherein by reference.

In an aspect, an RNA molecule is an antisense RNA.

In an aspect, at least a portion of an antisense RNA sequence is atleast 70%, at least 80%, at least 90%, at least 91%, at least 92%, atleast 93%, at least 94%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, or 100% complementary to a correspondingendogenous sequence of an RNA transcript. In an aspect, an antisense RNAsequence hybridizes to a corresponding endogenous sequence of an RNAtranscript. In an aspect, an antisense RNA sequence encoded by aninserted DNA segment hybridizes to a corresponding endogenous sequenceof an RNA transcript encoded by a corresponding endogenous DNA segment.In an aspect, an antisense RNA sequence forms a stem-loop structure witha corresponding endogenous sequence of an RNA transcript.

In an aspect, a mutant allele produces a RNA molecule comprising anantisense sequence that is at least 80%, at least 85%, at least 90%, atleast 91%, at least 92%, at least 93%, at least 94%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, or 100%complementary to a RNA transcript sequence, or a portion thereof,encoded by the endogenous GA20 oxidase_5 gene. In an aspect, an antisense sequence of an RNA molecule is at least 80%, at least 85%, atleast 90%, at least 91%, at least 92%, at least 93%, at least 94%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, or100% complementary to at least 15, at least 16, at least 17, at least18, at least 19, at least 20, at least 21, at least 22, at least 23, atleast 24, at least 25, at least 26, at least 27, at least 28, at least29, at least 30, at least 40, at least 50, at least 75, at least 100, orat least 200 consecutive nucleotides of one or more of SEQ ID NOs:218-220, 222-224, 226, and 228-255. In an aspect, an antisense sequenceof an RNA molecule is at least 80%, at least 85%, at least 90%, at least91%, at least 92%, at least 93%, at least 94%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, or 100% complementary toat least 15, at least 16, at least 17, at least 18, at least 19, atleast 20, at least 21, at least 22, at least 23, at least 24, at least25, at least 26, at least 27, at least 28, at least 29, at least 30, atleast 40, at least 50, at least 75, at least 100, or at least 200consecutive nucleotides of one or more of SEQ ID NOs: 222-224 and228-235.

In an aspect, an antisense sequence can hybridize with an RNA transcriptencoded by a wild-type allele of one or both of the endogenous GA20oxidase_3 gene and the endogenous GA20 oxidase_5 gene. In an aspect, anantisense sequence can hybridize with a sense RNA transcript encoded byan endogenous GA20 oxidase_5 gene. In an aspect, an antisense sequencecan hybridize with a sense RNA transcript encoded by the mutant alleleof the endogenous GA20 oxidase_5 gene. In an aspect, hybridizationbetween an antisense RNA and a sense RNA can cause suppression of awild-type or mutant allele of the endogenous GA20 oxidase_3 gene, awild-type or mutant allele of the endogenous GA20 oxidase_5 gene, or awild-type or mutant allele of both genes.

In an aspect, a sense RNA transcript encoded by the mutant allele of theendogenous GA20 oxidase_5 gene is shortened or truncated relative to awild-type allele of the endogenous GA20 oxidase_5 gene.

In an aspect, a mutant allele can suppress the expression of a wild-typeallele of the endogenous GA20 oxidase_3 locus, a wild-type allele of theendogenous GA20 oxidase_5 locus, or both.

In an aspect, an expression level(s) of one or more endogenous GA20oxidase and/or GA3 oxidase gene(s) is/are reduced or eliminated in thecorn plant, thereby suppressing the endogenous GA20 oxidase and/or GA3oxidase gene(s).

According to an aspect, a corn plant is provided having the expressionlevel(s) of one or more GA20 oxidase gene(s) reduced in at least oneplant tissue by at least 5%, at least 10%, at least 20%, at least 25%,at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, atleast 75%, at least 80%, at least 90%, or 100%, as compared to a controlcorn plant.

According to an aspect, a corn plant is provided having the expressionlevel(s) of one or more GA3 oxidase gene(s) reduced in at least oneplant tissue by at least 5%, at least 10%, at least 20%, at least 25%,at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, atleast 75%, at least 80%, at least 90%, or 100%, as compared to a controlcorn plant.

According to an aspect, a corn plant is provided having the expressionlevel(s) of one or more GA20 oxidase gene(s) reduced in at least oneplant tissue by 5%-20%, 5%-25%, 5%-30%, 5%-40%, 5%-50%, 5%-60%, 5%-70%,5%-75%, 5%-80%, 5%-90%, 5%-100%, 75%-100%, 50%-100%, 50%-90%, 50%-75%,25%-75%, 30%-80%, or 10%-75%, as compared to a control corn plant.

According to an aspect, a corn plant is provided having the expressionlevel(s) of one or more GA3 oxidase gene(s) reduced in at least oneplant tissue by 5%-20%, 5%-25%, 5%-30%, 5%-40%, 5%-50%, 5%-60%, 5%-70%,5%-75%, 5%-80%, 5%-90%, 5%-100%, 75%-100%, 50%-100%, 50%-90%, 50%-75%,25%-75%, 30%-80%, or 10%-75%, as compared to a control corn plant.

According to an aspect, the at least one tissue of a corn plant having areduced expression level of a GA20 oxidase and/or GA3 oxidase gene(s)includes one or more active GA producing tissue(s) of the plant, such asthe vascular and/or leaf tissue(s) of the plant, during one or morevegetative stage(s) of development.

In an aspect, suppression of an endogenous GA20 oxidase gene or a GA3oxidase gene is tissue-specific (e.g., only in leaf and/or vasculartissue). Suppression of a GA20 oxidase gene can be constitutive and/orvascular or leaf tissue specific or preferred. In other aspects,suppression of a GA20 oxidase gene or a GA3 oxidase gene is constitutiveand not tissue-specific. According to an aspect, expression of anendogenous GA20 oxidase gene and/or a GA3 oxidase gene is reduced in oneor more tissue types (e.g., in leaf and/or vascular tissue(s)) of amodified or transgenic plant as compared to the same tissue(s) of acontrol plant.

In an aspect, at least 10% of the corn plants in a field comprise amutation in a GA20 oxidase locus as compared to a wildtype GA20 oxidaselocus. In an aspect, at least 20% of the corn plants in a field comprisea mutation in a GA20 oxidase locus as compared to a wildtype GA20oxidase locus. In an aspect, at least 30% of the corn plants in a fieldcomprise a mutation in a GA20 oxidase locus as compared to a wildtypeGA20 oxidase locus. In an aspect, at least 40% of the corn plants in afield comprise a mutation in a GA20 oxidase locus as compared to awildtype GA20 oxidase locus. In an aspect, at least 50% of the cornplants in a field comprise a mutation in a GA20 oxidase locus ascompared to a wildtype GA20 oxidase locus. In an aspect, at least 60% ofthe corn plants in a field comprise a mutation in a GA20 oxidase locusas compared to a wildtype GA20 oxidase locus. In an aspect, at least 70%of the corn plants in a field comprise a mutation in a GA20 oxidaselocus as compared to a wildtype GA20 oxidase locus. In an aspect, atleast 80% of the corn plants in a field comprise a mutation in a GA20oxidase locus as compared to a wildtype GA20 oxidase locus. In anaspect, at least 90% of the corn plants in a field comprise a mutationin a GA20 oxidase locus as compared to a wildtype GA20 oxidase locus. Inan aspect, 100% of the corn plants in a field comprise a mutation in aGA20 oxidase locus as compared to a wildtype GA20 oxidase locus.

In an aspect, between 1% and 100% of the corn plants in a field comprisea mutation in a GA20 oxidase locus as compared to a wildtype GA20oxidase locus. In an aspect, between 10% and 100% of the corn plants ina field comprise a mutation in a GA20 oxidase locus as compared to awildtype GA20 oxidase locus. In an aspect, between 20% and 100% of thecorn plants in a field comprise a mutation in a GA20 oxidase locus ascompared to a wildtype GA20 oxidase locus. In an aspect, between 30% and100% of the corn plants in a field comprise a mutation in a GA20 oxidaselocus as compared to a wildtype GA20 oxidase locus. In an aspect,between 40% and 100% of the corn plants in a field comprise a mutationin a GA20 oxidase locus as compared to a wildtype GA20 oxidase locus. Inan aspect, between 50% and 100% of the corn plants in a field comprise amutation in a GA20 oxidase locus as compared to a wildtype GA20 oxidaselocus. In an aspect, between 60% and 100% of the corn plants in a fieldcomprise a mutation in a GA20 oxidase locus as compared to a wildtypeGA20 oxidase locus. In an aspect, between 70% and 100% of the cornplants in a field comprise a mutation in a GA20 oxidase locus ascompared to a wildtype GA20 oxidase locus. In an aspect, between 80% and100% of the corn plants in a field comprise a mutation in a GA20 oxidaselocus as compared to a wildtype GA20 oxidase locus. In an aspect,between 90% and 100% of the corn plants in a field comprise a mutationin a GA20 oxidase locus as compared to a wildtype GA20 oxidase locus.

In an aspect, at least 10% of the corn plants in a field comprise amutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 20% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 30% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 40% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 50% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 60% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 70% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 80% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, at least 90% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, 100% of the corn plants in a field comprise amutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus.

In an aspect, between 1% and 100% of the corn plants in a field comprisea mutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, between 10% and 100% of the corn plants in a fieldcomprise a mutation in a GA3 oxidase locus as compared to a wildtype GA3oxidase locus. In an aspect, between 20% and 100% of the corn plants ina field comprise a mutation in a GA3 oxidase locus as compared to awildtype GA3 oxidase locus. In an aspect, between 30% and 100% of thecorn plants in a field comprise a mutation in a GA3 oxidase locus ascompared to a wildtype GA3 oxidase locus. In an aspect, between 40% and100% of the corn plants in a field comprise a mutation in a GA3 oxidaselocus as compared to a wildtype GA3 oxidase locus. In an aspect, between50% and 100% of the corn plants in a field comprise a mutation in a GA3oxidase locus as compared to a wildtype GA3 oxidase locus. In an aspect,between 60% and 100% of the corn plants in a field comprise a mutationin a GA3 oxidase locus as compared to a wildtype GA3 oxidase locus. Inan aspect, between 70% and 100% of the corn plants in a field comprise amutation in a GA3 oxidase locus as compared to a wildtype GA3 oxidaselocus. In an aspect, between 80% and 100% of the corn plants in a fieldcomprise a mutation in a GA3 oxidase locus as compared to a wildtype GA3oxidase locus. In an aspect, between 90% and 100% of the corn plants ina field comprise a mutation in a GA3 oxidase locus as compared to awildtype GA3 oxidase locus.

In an aspect, at least 10% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA20oxidase gene or an mRNA transcribed therefrom. In an aspect, at least20% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA20 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 30% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA20 oxidase gene or an mRNA transcribed therefrom. Inan aspect, at least 40% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA20oxidase gene or an mRNA transcribed therefrom. In an aspect, at least50% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA20 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 60% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA20 oxidase gene or an mRNA transcribed therefrom. Inan aspect, at least 70% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA20oxidase gene or an mRNA transcribed therefrom. In an aspect, at least80% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA20 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 90% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA20 oxidase gene or an mRNA transcribed therefrom. Inan aspect, 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA20 oxidase geneor an mRNA transcribed therefrom.

In an aspect, between 1% and 100% of the corn plants in a field comprisea heterologous polynucleotide capable of suppressing expression of aGA20 oxidase gene or an mRNA transcribed therefrom. In an aspect,between 10% and 100% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA20oxidase gene or an mRNA transcribed therefrom. In an aspect, between 20%and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA20 oxidase geneor an mRNA transcribed therefrom. In an aspect, between 30% and 100% ofthe corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA20 oxidase gene or an mRNAtranscribed therefrom. In an aspect, between 40% and 100% of the cornplants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a GA20 oxidase gene or an mRNA transcribedtherefrom. In an aspect, between 50% and 100% of the corn plants in afield comprise a heterologous polynucleotide capable of suppressingexpression of a GA20 oxidase gene or an mRNA transcribed therefrom. Inan aspect, between 60% and 100% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA20oxidase gene or an mRNA transcribed therefrom. In an aspect, between 70%and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA20 oxidase geneor an mRNA transcribed therefrom. In an aspect, between 80% and 100% ofthe corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA20 oxidase gene or an mRNAtranscribed therefrom. In an aspect, between 90% and 100% of the cornplants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a GA20 oxidase gene or an mRNA transcribedtherefrom.

In an aspect, at least 10% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA3oxidase gene or an mRNA transcribed therefrom. In an aspect, at least20% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA3 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 30% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA3 oxidase gene or an mRNA transcribed therefrom. In anaspect, at least 40% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA3oxidase gene or an mRNA transcribed therefrom. In an aspect, at least50% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA3 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 60% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA3 oxidase gene or an mRNA transcribed therefrom. In anaspect, at least 70% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA3oxidase gene or an mRNA transcribed therefrom. In an aspect, at least80% of the corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA3 oxidase gene or an mRNAtranscribed therefrom. In an aspect, at least 90% of the corn plants ina field comprise a heterologous polynucleotide capable of suppressingexpression of a GA3 oxidase gene or an mRNA transcribed therefrom. In anaspect, 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA3 oxidase geneor an mRNA transcribed therefrom.

In an aspect, between 1% and 100% of the corn plants in a field comprisea heterologous polynucleotide capable of suppressing expression of a GA3oxidase gene or an mRNA transcribed therefrom. In an aspect, between 10%and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA3 oxidase geneor an mRNA transcribed therefrom. In an aspect, between 20% and 100% ofthe corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA3 oxidase gene or an mRNAtranscribed therefrom. In an aspect, between 30% and 100% of the cornplants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a GA3 oxidase gene or an mRNA transcribedtherefrom. In an aspect, between 40% and 100% of the corn plants in afield comprise a heterologous polynucleotide capable of suppressingexpression of a GA3 oxidase gene or an mRNA transcribed therefrom. In anaspect, between 50% and 100% of the corn plants in a field comprise aheterologous polynucleotide capable of suppressing expression of a GA3oxidase gene or an mRNA transcribed therefrom. In an aspect, between 60%and 100% of the corn plants in a field comprise a heterologouspolynucleotide capable of suppressing expression of a GA3 oxidase geneor an mRNA transcribed therefrom. In an aspect, between 70% and 100% ofthe corn plants in a field comprise a heterologous polynucleotidecapable of suppressing expression of a GA3 oxidase gene or an mRNAtranscribed therefrom. In an aspect, between 80% and 100% of the cornplants in a field comprise a heterologous polynucleotide capable ofsuppressing expression of a GA3 oxidase gene or an mRNA transcribedtherefrom. In an aspect, between 90% and 100% of the corn plants in afield comprise a heterologous polynucleotide capable of suppressingexpression of a GA3 oxidase gene or an mRNA transcribed therefrom.

Without being bound by theory, it is proposed that ectopic expression oroverexpression of GA2 oxidase transgene(s) may be effective in achievinga short stature, semi-dwarf phenotype with increased resistance tolodging, but without reproductive off-types in the ear. It is furtherproposed, without being limited by theory, that restricting theexpression of GA2 oxidase gene(s) to certain active GA-producingtissues, such as the vascular and/or leaf tissues of the plant, may besufficient to produce a short-stature plant with increased lodgingresistance, but without significant off-types in reproductive tissues.Expression of a GA2 oxidase transgene in a tissue-specific ortissue-preferred manner may be sufficient and effective at producingplants with the short stature phenotype, while avoiding potentialoff-types in reproductive tissues that were previously observed with GAmutants in corn (e.g., by avoiding or limiting the expression of the GA2oxidase gene(s) in those reproductive tissues). For example, the GA2oxidase transgene(s) may be expressed using a vascular promoter, such asa rice tungro bacilliform virus (RTBV) promoter, that drives expressionin vascular tissues of plants. The expression pattern of the RTBVpromoter is enriched in vascular tissues of corn plants relative tonon-vascular tissues, which is sufficient to produce a semi-dwarfphenotype in corn plants when operably linked to a transcribable DNAsequence encoding a GA2 oxidase gene(s). Lowering of active GA levels intissue(s) of a corn plant that produce active GAs may reduce plantheight and increase lodging resistance, and off-types may be avoided inthose plants if active GA levels are not also significantly impacted orlowered in reproductive tissues, such as the developing female organ orear of the plant. If active GA levels could be reduced in the stalk,stem, or internode(s) of corn plants without significantly affecting GAlevels in reproductive tissues (e.g., the female or male reproductiveorgans or inflorescences), then corn plants having reduced plant heightand increased lodging resistance could be created without off-types inthe reproductive tissues of the plant.

Thus, recombinant DNA constructs and transgenic plants are providedherein comprising a transcribable DNA sequence encoding a GA2 oxidasemRNA and protein operably linked to a plant expressible promoter, whichmay be a tissue-specific or tissue-preferred promoter. Such atissue-specific or tissue-preferred promoter may drive expression of itsassociated GA2 oxidase coding sequence in one or more activeGA-producing tissue(s) of the plant to reduce the level of active GAsproduced in those tissue(s). Such a tissue-specific or tissue-preferredpromoter may drive expression of its associated GA2 oxidase transgene orcoding sequence during one or more vegetative stage(s) of development.Such a tissue-specific or tissue-preferred promoter may also have littleor no expression in one or more cell(s) or tissue(s) of the developingfemale organ or ear of the plant to avoid the possibility of off-typesin those reproductive tissues. According to some embodiments, thetissue-specific or tissue-preferred promoter is a vascular promoter,such as the RTBV promoter. The sequence of the RTBV promoter is providedherein as SEQ ID NO: 656, and a truncated version of the RTBV promoteris further provided herein as SEQ ID NO: 657.

Active or bioactive gibberellic acids (i.e., “active gibberellins” or“active GAs”) are known in the art for a given plant species, asdistinguished from inactive GAs. For example, active GAs in corn andhigher plants include the following: GA1, GA3, GA4, and GA7. Thus, an“active GA-producing tissue” is a plant tissue that produces one or moreactive GAs.

In addition to suppressing GA20 oxidase genes in active GA-producingtissues of the plant with a vascular tissue promoter, it is furtherproposed that GA2 oxidase transgenes may also be expressed with variousconstitutive promoters to cause the short, semi-dwarf stature phenotypesin corn, without any visible off-types in the ear. Thus, it is furtherproposed that expression of one or more GA2 oxidase transgenes could becarried out using a constitutive promoter to create a short stature,lodging-resistant corn plant without any significant or observablereproductive off-types in the plant.

Without being limited by theory, it is proposed that short stature,semi-dwarf phenotypes in corn plants may result from a sufficient levelof expression of a GA2 oxidase transgene(s) in active GA-producingtissue(s) of the plant, and restricting the pattern of expression toavoid reproductive ear tissues may not be necessary to avoidreproductive off-types in the developing ear. It is proposed that thesemi-dwarf phenotype with GA2 oxidase overexpression can be the resultof shortening the stem internodes of the plant. Without being bound bytheory, it is proposed that expression of GA2 oxidase transgene(s) intissue(s) and/or cell(s) of the plant where active GAs are produced, andnot necessarily in stem or internode tissue(s), may be sufficient toproduce semi-dwarf plants, even though the short stature trait is due toshortening of the stem internodes. Given that GAs can migrate throughthe vasculature of the plant, it is proposed that manipulating GAoxidase genes in plant tissue(s) where active GAs are produced mayresult in a short stature, semi-dwarf plant, even though this may belargely achieved by reducing the level of active GAs produced innon-stem tissues (i.e., away from the site of action in the stem wherereduced internode elongation leads to the semi-dwarf phenotype).However, without being bound by theory, expression of a GA2 oxidasetransgene at low levels, and/or in a limited number of plant tissues,may be insufficient to cause a significant short stature, semi-dwarfphenotype.

The plant hormone gibberellin plays an important role in a number ofplant developmental processes including germination, cell elongation,flowering, embryogenesis and seed development. Certain biosyntheticenzymes (e.g., GA20 oxidase and GA3 oxidase) and catabolic enzymes(e.g., GA2 oxidase) in the GA pathway are critical to affecting activeGA levels in plant tissues. While the biosynthetic enzymes can increasethe level of active GAs, the catabolic enzymes can reduce the level(s)of active GAs in plants or plant cells. Thus, it is proposed thatoverexpression or ectopic expression of a GA2 oxidase transgene in aconstitutive or tissue-specific or tissue-preferred manner may producecorn plants having a short stature phenotype and increased lodgingresistance, with possible increased yield, but without off-types in theear. Thus, according to some embodiments, constructs and transgenes areprovided comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein operably linked to a constitutive or tissue-specific ortissue-preferred promoter, such as a vascular or leaf promoter.According to some embodiments, the tissue-specific or tissue-preferredpromoter is a vascular promoter, such as the RTBV promoter. However,other types of tissue-specific or tissue preferred promoters maypotentially be used for GA2 oxidase expression in active GA-producingtissues of a corn plant to produce a semi-dwarf phenotype withoutsignificant off-types.

According to some embodiments, a modified or transgenic plant isprovided having a GA2 oxidase gene expression level that is increased inat least one plant tissue by at least 5%, at least 10%, at least 20%, atleast 25%, at least 30%, at least 40%, at least 50%, at least 60%, atleast 70%, at least 75%, at least 80%, at least 90%, or 100%, ascompared to a control plant. According to some embodiments, a modifiedor transgenic plant is provided having a GA2 oxidase gene expressionlevel that is increased in at least one plant tissue by 5%-20%, 5%-25%,5%-30%, 5%-40%, 5%-50%, 5%-60%, 5%-70%, 5%-75%, 5%-80%, 5%-90%, 5%-100%,75%-100%, 50%-100%, 50%-90%, 50%-75%, 25%-75%, 30%-80%, or 10%-75%, ascompared to a control plant. According to these embodiments, the atleast one tissue of a modified or transgenic plant having an increasedexpression level of a GA2 oxidase gene(s) includes one or more active GAproducing tissue(s) of the plant, such as the vascular and/or leaftissue(s) of the plant, during one or more vegetative stage(s) ofdevelopment.

In some embodiments, transgenic expression of a GA2 oxidase transgene isconstitutive or tissue-specific (e.g., only in leaf and/or vasculartissue). For example, expression of a GA2 oxidase transgene may bevascular or leaf tissue specific or preferred. In other embodiments,expression of a GA2 oxidase transgene is constitutive and nottissue-specific. According to some embodiments, expression of a GA2oxidase transgene is increased in one or more tissue types (e.g., inleaf and/or vascular tissue(s)) of a modified or transgenic plant ascompared to the same tissue(s) of a control plant.

According to embodiments of the present disclosure, a recombinant DNAmolecule, construct or vector is provided comprising an expressioncassette comprising a GA2 oxidase coding sequence or transcribable DNAsequence that is operably linked to a plant-expressible constitutive ortissue-specific or tissue-preferred promoter. The expression cassettemay comprise a transcribable DNA sequence having a percent identity toall or part of a GA2 oxidase gene or coding sequence. A transgene havinga coding sequence with a lower percent identity to all or part of a GA2oxidase gene may encode a protein having or retaining a GA catabolicactivity in a corn plant or plant cell similar to GA2 oxidase genes ingeneral.

A single GA2 oxidase transgene or expression cassette may be present ina construct, molecule or vector, or multiple GA2 oxidase transgenes orexpression cassettes may be arranged serially in tandem or arranged intandem segments or repeats, in a construct, molecule or vector, whichmay also be interrupted by one or more spacer sequence(s). The sequenceof each transgene or expression cassette may encode a GA2 oxidase mRNAand protein. A transcribable DNA sequence or coding sequence of a GA2oxidase transgene may encode a protein having at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5% or 100%identity to all or part of a GA2 oxidase gene sequence.

According to embodiments of the present disclosure, a recombinant DNAmolecule, construct or vector is provided comprising a transcribable DNAsequence encoding a GA2 oxidase. According to some embodiments, arecombinant DNA molecule, vector or construct is provided comprising atranscribable DNA sequence encoding a GA2 oxidase mRNA and protein in aplant cell, and wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter, such as a constitutive ortissue-specific or tissue-preferred promoter. According to embodimentsof the present disclosure, suitable tissue-specific or tissue preferredpromoters for expression of a GA2 oxidase may include those promotersthat drive or cause expression of its associated suppression element orsequence at least in the vascular and/or leaf tissue(s) of a corn plant.Expression of the GA2 oxidase with a tissue-specific or tissue-preferredpromoter may also occur in other tissues of the corn plant outside ofthe vascular and leaf tissues, but active GA levels in the developingreproductive tissues of the plant (particularly in the femalereproductive organ or ear) are preferably not significantly reduced orimpacted (relative to wild type or control plants), such thatdevelopment of the female organ or ear may proceed normally in thetransgenic plant without off-types in the ear and a loss in yieldpotential. According to many embodiments, the plant-expressible promotermay preferably drive expression constitutively or in at least a portionof the vascular and/or leaf tissues of the plant. However, sometissue-specific and tissue-preferred promoters driving expression of aGA2 oxidase transgene in a plant may not produce a significant shortstature or anti-lodging phenotypes due to the spatial-temporal patternof expression of the promoter during plant development, and/or theamount or strength of expression of the promoter being too low or weak.A sufficient level of expression of a transcribable DNA sequenceencoding a GA2 oxidase may be necessary to produce a short stature,semi-dwarf phenotype that resists lodging, since lower levels ofexpression may be insufficient to lower active GA levels in the plant toa sufficient extent to cause a significant phenotype. Thus,tissue-specific and tissue-preferred promoters that drive, etc., amoderate or strong level of expression of their associated transcribableDNA sequence in active GA-producing tissue(s) of a plant may bepreferred. Furthermore, such tissue-specific and tissue-preferred shoulddrive, etc., expression of their associated transcribable DNA sequenceduring one or more vegetative stage(s) of plant development when theplant is growing and/or elongating including one or more of thefollowing vegetative stage(s): V_(E), V1, V2, V3, V4, V5, V6, V7, V8,V9, V10, V11, V12, V13, V14, Vn, V_(T), such as expression at leastduring V3-V12, V4-V12, V5-V12, V6-V12, V7-V12, V8-V12, V3-V14, V5-V14,V6-V14, V7-V14, V8-V14, V9-V14, V10-V14, etc., or during any other rangeof vegetative stages when growth and/or elongation of the plant isoccurring.

Any vascular promoters known in the art may potentially be used as thetissue-specific or tissue-preferred promoter. Examples of vascularpromoters include the RTBV promoter (see, e.g., SEQ ID NO: 656), a knownsucrose synthase gene promoter, such as a corn sucrose synthase-1 (Sus1or Sh1) promoter (see, e.g., SEQ ID NO: 658), a corn Sh1 gene paralogpromoter, a barley sucrose synthase promoter (Ss1) promoter, a ricesucrose synthase-1 (RSs1) promoter (see, e.g., SEQ ID NO: 659), or arice sucrose synthase-2 (RSs2) promoter (see, e.g., SEQ ID NO: 660), aknown sucrose transporter gene promoter, such as a rice sucrosetransporter promoter (SUT1) (see, e.g., SEQ ID NO: 661), or variousknown viral promoters, such as a Commelina yellow mottle virus (CoYMV)promoter, a wheat dwarf geminivirus (WDV) large intergenic region (LIR)promoter, a maize streak geminivirus (MSV) coat protein (CP) promoter,or a rice yellow stripe 1 (YS1)-like or OsYSL2 promoter (SEQ ID NO:662), and any functional sequence portion or truncation of any of theforegoing promoters with a similar pattern of expression, such as atruncated RTBV promoter (see, e.g., SEQ ID NO: 657). Any other vascularpromoters known in the art may also be used, including promotersequences from related genes (e.g., sucrose synthase, sucrosetransporter, and viral gene promoter sequences) from the same ordifferent plant species, microbe or virus that have a similar pattern ofexpression. Further provided are promoter sequences with a high degreeof homology to any of the foregoing. For example, a vascular promotermay comprise a DNA sequence that is at least at least 70%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5% or 100% identical to one or moreof SEQ ID NOs: 656, 657, 658, 659, 660, 661, and 662, any functionalsequence portion or truncation thereof, and/or any sequencecomplementary to any of the foregoing sequences. Examples of vascularpromoters may further include other known, engineered and/orlater-identified promoter sequences shown to have a pattern ofexpression in vascular tissue(s) of a corn plant.

Any leaf promoters known in the art may potentially be used as thetissue-specific or tissue-preferred promoter. Examples of leaf promotersinclude a corn pyruvate phosphate dikinase or PPDK promoter (see, e.g.,SEQ ID NO: 663), a corn fructose 1,6 bisphosphate aldolase or FDApromoter (see, e.g., SEQ ID NO: 664), and a rice Nadh-Gogat promoter(see, e.g., SEQ ID NO: 665), and any functional sequence portion ortruncation of any of the foregoing promoters with a similar pattern ofexpression. Other examples of leaf promoters from monocot plant genesinclude a ribulose biphosphate carboxylase (RuBisCO) or RuBisCO smallsubunit (RBCS) promoter, a chlorophyll a/b binding protein genepromoter, a phosphoenolpyruvate carboxylase (PEPC) promoter, and a Mybgene promoter, and any functional sequence portion or truncation of anyof these promoters with a similar pattern of expression. Any other leafpromoters known in the art may also be used, including promotersequences from related genes from the same or different plant species,microbe or virus that have a similar pattern of expression. Furtherprovided are promoter sequences with a high degree of homology to any ofthe foregoing. For example, a leaf promoter may comprise a DNA sequencethat is at least at least 70%, at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5% or 100% identical to one or more of SEQ ID NOs: 663, 664,and 665, any functional sequence portion or truncation thereof, and/orany sequence complementary to any of the foregoing sequences. Examplesof leaf promoters may further include other known, engineered and/orlater-identified promoter sequences shown to have a pattern ofexpression in leaf tissue(s) of a corn plant.

Any constitutive promoters known in the art may potentially be used.Examples of constitutive promoters that may be used in corn plantsinclude, for example, various actin gene promoters, such as a rice Actin1 promoter (see, e.g., U.S. Pat. No. 5,641,876; see also SEQ ID NO: 666or SEQ ID NO: 667) and a rice Actin 2 promoter (see, e.g., U.S. Pat. No.6,429,357; see also, e.g., SEQ ID NO: 668 or SEQ ID NO: 669), a CaMV 35Sor 19S promoter (see, e.g., U.S. Pat. No. 5,352,605; see also, e.g., SEQID NO: 670 for CaMV 35S), a maize ubiquitin promoter (see, e.g., U.S.Pat. No. 5,510,474), a Coix lacryma-jobi polyubiquitin promoter (see,e.g., SEQ ID NO: 671), a rice or maize Gos2 promoter (see, e.g., Pateret al., The Plant Journal, 2(6): 837-44 1992; see also, e.g., SEQ ID NO:672 for the rice Gos2 promoter), a FMV 35S promoter (see, e.g., U.S.Pat. No. 6,372,211), a dual enhanced CMV promoter (see, e.g., U.S. Pat.No. 5,322,938), a MMV promoter (see, e.g., U.S. Pat. No. 6,420,547; seealso, e.g., SEQ ID NO: 673), a PCLSV promoter (see, e.g., U.S. Pat. No.5,850,019; see also, e.g., SEQ ID NO: 674), an Emu promoter (see, e.g.,Last et al., Theor. Appl. Genet. 81:581 (1991); and Mcelroy et al., Mol.Gen. Genet. 231:150 (1991)), a tubulin promoter from maize, rice orother species, a nopaline synthase (nos) promoter, an octopine synthase(ocs) promoter, a mannopine synthase (mas) promoter, or a plant alcoholdehydrogenase (e.g., maize Adhl) promoter, any other promoters includingviral promoters known or later-identified in the art to provideconstitutive expression in a corn plant, any other constitutivepromoters known in the art that may be used in corn plants, and anyfunctional sequence portion or truncation of any of the foregoingpromoters.

Any other constitutive promoters known in the art may also be used,including promoter sequences from related genes from the same ordifferent plant species, microbe or virus that have a similar pattern ofexpression. Further provided are promoter sequences with a high degreeof homology to any of the foregoing. For example, a constitutivepromoter may comprise a DNA sequence that is at least at least 70%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5% or 100% identicalto one or more of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673,and 674, any functional sequence portion or truncation thereof, and/orany sequence complementary to any of the foregoing sequences. Examplesof constitutive promoters may further include other known, engineeredand/or later-identified promoter sequences shown to have a constitutivepattern of expression in a corn plant. Furthermore, any known orlater-identified constitutive promoter may also be used.

According to embodiments of the present disclosure, a recombinant DNAmolecule, construct or vector is provided comprising a transcribable DNAsequence encoding a GA2 oxidase protein from a monocot or cereal plant,such as a corn plant, or encoding a GA2 oxidase protein having at leasta certain percent homology to a GA2 oxidase protein from a monocot orcereal plant, such as a corn plant. A family of at least thirteen GA2oxidase genes have been identified in corn (Zea mays) including Zm.GA2oxidase_1, Zm.GA2 oxidase_2, Zm.GA2 oxidase_3, Zm.GA2 oxidase_4, Zm.GA2oxidase_5, Zm.GA2 oxidase_6, Zm.GA2 oxidase_7, Zm.GA2 oxidase_8, Zm.GA2oxidase_9, Zm.GA2 oxidase_10, Zm.GA2 oxidase_11, Zm.GA2 oxidase_12, andZm.GA2 oxidase_13. The DNA and protein sequences by SEQ ID NO for eachof these GA2 oxidase genes are provided in Table 3.

TABLE 3 DNA and protein sequences for GA2 oxidase genes in corn. CodingSequence GA2 oxidase Gene (CDS) Protein Zm.GA2 oxidase_1 SEQ ID NO: 324SEQ ID NO: 325 Zm.GA2 oxidase_2 SEQ ID NO: 326 SEQ ID NO: 327 Zm.GA2oxidase_3 SEQ ID NO: 328 SEQ ID NO: 329 Zm.GA2 oxidase_4 SEQ ID NO: 330SEQ ID NO: 331 Zm.GA2 oxidase_5 SEQ ID NO: 332 SEQ ID NO: 333 Zm.GA2oxidase_6 SEQ ID NO: 334 SEQ ID NO: 335 Zm.GA2 oxidase_7 SEQ ID NO: 336SEQ ID NO: 337 Zm.GA2 oxidase_8 SEQ ID NO: 338 SEQ ID NO: 339 Zm.GA2oxidase_9 SEQ ID NO: 340 SEQ ID NO: 341 Zm.GA2 oxidase_10 SEQ ID NO: 342SEQ ID NO: 343 Zm.GA2 oxidase_11 SEQ ID NO: 344 SEQ ID NO: 345 Zm.GA2oxidase_12 SEQ ID NO: 346 SEQ ID NO: 347 Zm.GA2 oxidase_13 SEQ ID NO:348 SEQ ID NO: 349

GA2 oxidase genes from other monocot or cereal plant species may also beused, such as rice, barley, wheat and sorghum. According to embodimentsof the present disclosure, a recombinant DNA molecule, construct orvector is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein from a monocot or cereal plant other than corn, orencoding a GA2 oxidase protein having at least a certain percenthomology to a GA2 oxidase protein from a monocot or cereal plant otherthan corn. A family of at least ten GA2 oxidase genes have beenidentified in rice (Oryza sativa) plants including Os.GA2 oxidase_1,Os.GA2 oxidase_2, Os.GA2 oxidase_3, Os.GA2 oxidase_4, Os.GA2 oxidase_5,Os.GA2 oxidase_6, Os.GA2 oxidase_7, Os.GA2 oxidase_8, Os.GA2 oxidase_9,and Os.GA2 oxidase_10. The DNA and protein sequences by SEQ ID NO foreach of these GA2 oxidase genes are provided in Table 4.

TABLE 4 DNA and protein sequences for GA2 oxidase genes in rice. CodingSequence GA2 oxidase Gene (CDS) Protein Os.GA2 oxidase_1 SEQ ID NO: 350SEQ ID NO: 351 Os.GA2 oxidase_2 SEQ ID NO: 352 SEQ ID NO: 353 Os.GA2oxidase_3 SEQ ID NO: 354 SEQ ID NO: 355 Os.GA2 oxidase_4 SEQ ID NO: 356SEQ ID NO: 357 Os.GA2 oxidase_5 SEQ ID NO: 358 SEQ ID NO: 359 Os.GA2oxidase_6 SEQ ID NO: 360 SEQ ID NO: 361 Os.GA2 oxidase_7 SEQ ID NO: 362SEQ ID NO: 363 Os.GA2 oxidase_8 SEQ ID NO: 364 SEQ ID NO: 365 Os.GA2oxidase_9 SEQ ID NO: 366 SEQ ID NO: 367 Os.GA2 oxidase_10 SEQ ID NO: 368SEQ ID NO: 369

A family of at least eight GA2 oxidase genes have been identified inbarley (Hordeum vulgare) plants including Hv.GA2 oxidase_1, Hv.GA2oxidase_2, Hv.GA2 oxidase_3, Hv.GA2 oxidase_4, Hv.GA2 oxidase_5, Hv.GA2oxidase_6, Hv.GA2 oxidase_7, and Hv.GA2 oxidase_8. The DNA and proteinsequences by SEQ ID NO for each of these GA2 oxidase genes are providedin Table 5.

TABLE 5 DNA and protein sequences for GA2 oxidase genes in barley.Coding Sequence GA2 oxidase Gene (CDS) Protein Hv.GA2 oxidase_1 SEQ IDNO: 370 SEQ ID NO: 371 Hv.GA2 oxidase_2 SEQ ID NO: 372 SEQ ID NO: 373Hv.GA2 oxidase_3 SEQ ID NO: 374 SEQ ID NO: 375 Hv.GA2 oxidase_4 SEQ IDNO: 376 SEQ ID NO: 377 Hv.GA2 oxidase_5 SEQ ID NO: 378 SEQ ID NO: 379Hv.GA2 oxidase_6 SEQ ID NO: 380 SEQ ID NO: 381 Hv.GA2 oxidase_7 SEQ IDNO: 382 SEQ ID NO: 383 Hv.GA2 oxidase_8 SEQ ID NO: 384 SEQ ID NO: 385

A family of at least sixteen GA2 oxidase genes have been identified insorghum (Sorghum bicolor) plants including Hv.GA2 oxidase_1, Sb.GA2oxidase_2, Sb.GA2 oxidase_3, Sb.GA2 oxidase_4, Sb.GA2 oxidase_5, Sb.GA2oxidase_6, Sb.GA2 oxidase_7, Sb.GA2 oxidase_8, Sb.GA2 oxidase_9, Sb.GA2oxidase_10, Sb.GA2 oxidase_11, Sb.GA2 oxidase_12, Sb.GA2 oxidase_13,Sb.GA2 oxidase_14, Sb.GA2 oxidase_15, and Sb.GA2 oxidase_16. The DNA andprotein sequences by SEQ ID NO for each of these GA2 oxidase genes areprovided in Table 6.

TABLE 6 DNA and protein sequences for GA2 oxidase genes in sorghum.Coding Sequence GA2 oxidase Gene (CDS) Protein Sb.GA2 oxidase_1 SEQ IDNO: 386 SEQ ID NO: 387 Sb.GA2 oxidase_2 SEQ ID NO: 388 SEQ ID NO: 389Sb.GA2 oxidase_3 SEQ ID NO: 390 SEQ ID NO: 391 Sb.GA2 oxidase_4 SEQ IDNO: 392 SEQ ID NO: 393 Sb.GA2 oxidase_5 SEQ ID NO: 394 SEQ ID NO: 395Sb.GA2 oxidase_6 SEQ ID NO: 396 SEQ ID NO: 397 Sb.GA2 oxidase_7 SEQ IDNO: 398 SEQ ID NO: 399 Sb.GA2 oxidase_8 SEQ ID NO: 400 SEQ ID NO: 401Sb.GA2 oxidase_9 SEQ ID NO: 402 SEQ ID NO: 403 Sb.GA2 oxidase_10 SEQ IDNO: 404 SEQ ID NO: 405 Sb.GA2 oxidase_11 SEQ ID NO: 406 SEQ ID NO: 407Sb.GA2 oxidase_12 SEQ ID NO: 408 SEQ ID NO: 409 Sb.GA2 oxidase_13 SEQ IDNO: 410 SEQ ID NO: 411 Sb.GA2 oxidase_14 SEQ ID NO: 412 SEQ ID NO: 413Sb.GA2 oxidase_15 SEQ ID NO: 414 SEQ ID NO: 415 Sb.GA2 oxidase_16 SEQ IDNO: 416 SEQ ID NO: 417

A family of at least fifteen GA2 oxidase genes have been identified inwheat (Triticum aestivum) including Ta.GA2 oxidase_1, Ta.GA2 oxidase_2,Ta.GA2 oxidase_3, Ta.GA2 oxidase_4, Ta.GA2 oxidase_5, Ta.GA2 oxidase_6,Ta.GA2 oxidase_7, Ta.GA2 oxidase_8, Ta.GA2 oxidase_9, Ta.GA2 oxidase_10,Ta.GA2 oxidase_11, Ta.GA2 oxidase_12, Ta.GA2 oxidase_13, Ta.GA2oxidase_14, and Ta.GA2 oxidase_15. The DNA and protein sequences by SEQID NO for each of these GA2 oxidase genes from wheat are provided inTable 7.

TABLE 7 DNA and protein sequences for GA2 oxidase genes in wheat. CodingSequence GA2 oxidase Gene (CDS) Protein Ta.GA2 oxidase_1 SEQ ID NO: 418SEQ ID NO: 419 Ta.GA2 oxidase_2 SEQ ID NO: 420 SEQ ID NO: 421 Ta.GA2oxidase_3 SEQ ID NO: 422 SEQ ID NO: 423 Ta.GA2 oxidase_4 SEQ ID NO: 424SEQ ID NO: 425 Ta.GA2 oxidase_5 SEQ ID NO: 426 SEQ ID NO: 427 Ta.GA2oxidase_6 SEQ ID NO: 428 SEQ ID NO: 429 Ta.GA2 oxidase_7 SEQ ID NO: 430SEQ ID NO: 431 Ta.GA2 oxidase_8 SEQ ID NO: 432 SEQ ID NO: 433 Ta.GA2oxidase_9 SEQ ID NO: 434 SEQ ID NO: 435 Ta.GA2 oxidase_10 SEQ ID NO: 436SEQ ID NO: 437 Ta.GA2 oxidase_11 SEQ ID NO: 438 SEQ ID NO: 439 Ta.GA2oxidase_12 SEQ ID NO: 440 SEQ ID NO: 441 Ta.GA2 oxidase_13 SEQ ID NO:442 SEQ ID NO: 443 Ta.GA2 oxidase_14 SEQ ID NO: 444 SEQ ID NO: 445Ta.GA2 oxidase_15 SEQ ID NO: 446 SEQ ID NO: 447

In addition to the corn sequences listed in Table 3, a family of atleast eleven GA2 oxidase genes have been identified in another corn (Zeamays) germplasm line including Zm2.GA2 oxidase_1, Zm2.GA2 oxidase_2,Zm2.GA2 oxidase_3, Zm2.GA2 oxidase_4, Zm2.GA2 oxidase_5, Zm2.GA2oxidase_6, Zm2.GA2 oxidase_7, Zm2.GA2 oxidase_8, Zm2.GA2 oxidase_9,Zm2.GA2 oxidase_10, and Zm2.GA2 oxidase_11. The DNA and proteinsequences by SEQ ID NO for each of these GA2 oxidase genes are providedin Table 8.

TABLE 8 Additional DNA and protein sequences for GA2 oxidase genes incorn. Coding Sequence GA2 oxidase Gene (CDS) Protein Zm2.GA2 oxidase_1SEQ ID NO: 448 SEQ ID NO: 449 Zm2.GA2 oxidase_2 SEQ ID NO: 450 SEQ IDNO: 451 Zm2.GA2 oxidase_3 SEQ ID NO: 452 SEQ ID NO: 453 Zm2.GA2oxidase_4 SEQ ID NO: 454 SEQ ID NO: 455 Zm2.GA2 oxidase_5 SEQ ID NO: 456SEQ ID NO: 457 Zm2.GA2 oxidase_6 SEQ ID NO: 458 SEQ ID NO: 459 Zm2.GA2oxidase_7 SEQ ID NO: 460 SEQ ID NO: 461 Zm2.GA2 oxidase_8 SEQ ID NO: 462SEQ ID NO: 463 Zm2.GA2 oxidase_9 SEQ ID NO: 464 SEQ ID NO: 465 Zm2.GA2oxidase_10 SEQ ID NO: 466 SEQ ID NO: 467 Zm2.GA2 oxidase_11 SEQ ID NO:468 SEQ ID NO: 469

According to embodiments of the present disclosure, a recombinant DNAmolecule, construct or vector is provided comprising a transcribable DNAsequence encoding a GA2 oxidase protein from a dicot plant, such as asoybean, cotton, canola, Arabidopsis, moss (Physcomitrella patens),common bean (Phaseolus vulgaris), cottonwood (Populus trichocarpa),barrel clover (Medicago truncatula), pea (Pisum sativum), spinach(Spinacia oleracea) or whorled honey flower (Paris polyphylla) plant, orencoding a GA2 oxidase protein having at least a certain percenthomology to a GA2 oxidase protein from a monocot or cereal plant, suchas a corn plant.

A family of at least sixteen GA2 oxidase genes have been identified insoybean (Glycine max) including Gm.GA2 oxidase_1, Gm.GA2 oxidase_2,Gm.GA2 oxidase_3, Gm.GA2 oxidase_4, Gm.GA2 oxidase_5, Gm.GA2 oxidase_6,Gm.GA2 oxidase_7, Gm.GA2 oxidase_8, Gm.GA2 oxidase_9, Gm.GA2 oxidase_10,Gm.GA2 oxidase_11, Gm.GA2 oxidase_12, Gm.GA2 oxidase_13, Gm.GA2oxidase_14, Gm.GA2 oxidase_15, and Gm.GA2 oxidase_16. The DNA andprotein sequences by SEQ ID NO for each of these GA2 oxidase genes fromsoybean are provided in Table 9.

TABLE 9 Additional DNA and protein sequences for GA2 oxidase genes insoybean. Coding Sequence GA2 oxidase Gene (CDS) Protein Gm.GA2 oxidase_1SEQ ID NO: 470 SEQ ID NO: 471 Gm.GA2 oxidase_2 SEQ ID NO: 472 SEQ ID NO:473 Gm.GA2 oxidase_3 SEQ ID NO: 474 SEQ ID NO: 475 Gm.GA2 oxidase_4 SEQID NO: 476 SEQ ID NO: 477 Gm.GA2 oxidase_5 SEQ ID NO: 478 SEQ ID NO: 479Gm.GA2 oxidase_6 SEQ ID NO: 480 SEQ ID NO: 481 Gm.GA2 oxidase_7 SEQ IDNO: 482 SEQ ID NO: 483 Gm.GA2 oxidase_8 SEQ ID NO: 484 SEQ ID NO: 485Gm.GA2 oxidase_9 SEQ ID NO: 486 SEQ ID NO: 487 Gm.GA2 oxidase_10 SEQ IDNO: 488 SEQ ID NO: 489 Gm.GA2 oxidase_11 SEQ ID NO: 490 SEQ ID NO: 491Gm.GA2 oxidase_12 SEQ ID NO: 492 SEQ ID NO: 493 Gm.GA2 oxidase_13 SEQ IDNO: 494 SEQ ID NO: 495 Gm.GA2 oxidase_14 SEQ ID NO: 496 SEQ ID NO: 497Gm.GA2 oxidase_15 SEQ ID NO: 498 SEQ ID NO: 499 Gm.GA2 oxidase_16 SEQ IDNO: 500 SEQ ID NO: 501

A family of at least fifteen related GA2 oxidase genes have beenidentified in cotton (Gossypium hirsutum) including Gh.GA2 oxidase_1,Gh.GA2 oxidase_2, Gh.GA2 oxidase_3, Gh.GA2 oxidase_4, Gh.GA2 oxidase_5,Gh.GA2 oxidase_6, Gh.GA2 oxidase_7, Gh.GA2 oxidase_8, Gh.GA2 oxidase_9,Gh.GA2 oxidase_10, Gh.GA2 oxidase_11, Gh.GA2 oxidase_12, Gh.GA2oxidase_13, Gh.GA2 oxidase_14, and Gh.GA2 oxidase_15. The DNA andprotein sequences by SEQ ID NO for each of these GA2 oxidase genes fromcotton are provided in Table 10.

TABLE 10 DNA and protein sequences for GA2 oxidase genes in cotton.Coding Sequence GA2 oxidase Gene (CDS) Protein Gh.GA2 oxidase_1 SEQ IDNO: 502 SEQ ID NO: 503 Gh.GA2 oxidase_2 SEQ ID NO: 504 SEQ ID NO: 505Gh.GA2 oxidase_3 SEQ ID NO: 506 SEQ ID NO: 507 Gh.GA2 oxidase_4 SEQ IDNO: 508 SEQ ID NO: 509 Gh.GA2 oxidase_5 SEQ ID NO: 510 SEQ ID NO: 511Gh.GA2 oxidase_6 SEQ ID NO: 512 SEQ ID NO: 513 Gh.GA2 oxidase_7 SEQ IDNO: 514 SEQ ID NO: 515 Gh.GA2 oxidase_8 SEQ ID NO: 516 SEQ ID NO: 517Gh.GA2 oxidase_9 SEQ ID NO: 518 SEQ ID NO: 519 Gh.GA2 oxidase_10 SEQ IDNO: 520 SEQ ID NO: 521 Gh.GA2 oxidase_11 SEQ ID NO: 522 SEQ ID NO: 523Gh.GA2 oxidase_12 SEQ ID NO: 524 SEQ ID NO: 525 Gh.GA2 oxidase_13 SEQ IDNO: 526 SEQ ID NO: 527 Gh.GA2 oxidase_14 SEQ ID NO: 528 SEQ ID NO: 529Gh.GA2 oxidase_15 SEQ ID NO: 530 SEQ ID NO: 531

A family of at least fifteen GA2 oxidase genes have been identified incanola (Brassica napus) including Bn.GA2 oxidase_1, Bn.GA2 oxidase_2,Bn.GA2 oxidase_3, Bn.GA2 oxidase_4, Bn.GA2 oxidase_5, Bn.GA2 oxidase_6,Bn.GA2 oxidase_7, Bn.GA2 oxidase_8, Bn.GA2 oxidase_9, Bn.GA2 oxidase_10,Bn.GA2 oxidase_11, Bn.GA2 oxidase_12, Bn.GA2 oxidase_13, Bn.GA2oxidase_14, and Bn.GA2 oxidase_15. The DNA and protein sequences by SEQID NO for each of these GA2 oxidase genes from canola are provided inTable 11.

TABLE 11 DNA and protein sequences for GA2 oxidase genes in canola.Coding Sequence GA2 oxidase Gene (CDS) Protein Bn.GA2 oxidase_1 SEQ IDNO: 532 SEQ ID NO: 533 Bn.GA2 oxidase_2 SEQ ID NO: 534 SEQ ID NO: 535Bn.GA2 oxidase_3 SEQ ID NO: 536 SEQ ID NO: 537 Bn.GA2 oxidase_4 SEQ IDNO: 538 SEQ ID NO: 539 Bn.GA2 oxidase_5 SEQ ID NO: 540 SEQ ID NO: 541Bn.GA2 oxidase_6 SEQ ID NO: 542 SEQ ID NO: 543 Bn.GA2 oxidase_7 SEQ IDNO: 544 SEQ ID NO: 545 Bn.GA2 oxidase_8 SEQ ID NO: 546 SEQ ID NO: 547Bn.GA2 oxidase_9 SEQ ID NO: 548 SEQ ID NO: 549 Bn.GA2 oxidase_10 SEQ IDNO: 550 SEQ ID NO: 551 Bn.GA2 oxidase_11 SEQ ID NO: 552 SEQ ID NO: 553Bn.GA2 oxidase_12 SEQ ID NO: 554 SEQ ID NO: 555 Bn.GA2 oxidase_13 SEQ IDNO: 556 SEQ ID NO: 557 Bn.GA2 oxidase_14 SEQ ID NO: 558 SEQ ID NO: 559Bn.GA2 oxidase_15 SEQ ID NO: 560 SEQ ID NO: 561

A family of at least seven GA2 oxidase genes have been identified inthale cress (Arabidopsis thaliana) including At.GA2 oxidase_1, At.GA2oxidase_2, At.GA2 oxidase_3, At.GA2 oxidase_4, At.GA2 oxidase_6, andAt.GA2 oxidase_7, and At.GA2 oxidase_8. The DNA and protein sequences bySEQ ID NO for each of these GA2 oxidase genes from Arabidopsis areprovided in Table 12.

TABLE 12 DNA and protein sequences for GA2 oxidase genes in thale cress.Coding Sequence GA2 oxidase Gene (CDS) Protein At.GA2 oxidase_1 SEQ IDNO: 562 SEQ ID NO: 563 At.GA2 oxidase_2 SEQ ID NO: 564 SEQ ID NO: 565At.GA2 oxidase_3 SEQ ID NO: 566 SEQ ID NO: 567 At.GA2 oxidase_4 SEQ IDNO: 568 SEQ ID NO: 569 At.GA2 oxidase_6 SEQ ID NO: 570 SEQ ID NO: 571At.GA2 oxidase_7 SEQ ID NO: 572 SEQ ID NO: 573 At.GA2 oxidase_8 SEQ IDNO: 574 SEQ ID NO: 575

A family of at least seven GA2 oxidase genes have been identified inmoss (Physcomitrella patens) including Pp.GA2 oxidase_1, Pp.GA2oxidase_2, Pp.GA2 oxidase_3, Pp.GA2 oxidase_4, Pp.GA2 oxidase_5, Pp.GA2oxidase_6, and Pp.GA2 oxidase_7. The DNA and protein sequences by SEQ IDNO for each of these GA2 oxidase genes from moss are provided in Table13.

TABLE 13 DNA and protein sequences for GA2 oxidase genes in moss. CodingSequence GA2 oxidase Gene (CDS) Protein Pp.GA2 oxidase_1 SEQ ID NO: 576SEQ ID NO: 577 Pp.GA2 oxidase_2 SEQ ID NO: 578 SEQ ID NO: 579 Pp.GA2oxidase_3 SEQ ID NO: 580 SEQ ID NO: 581 Pp.GA2 oxidase_4 SEQ ID NO: 582SEQ ID NO: 583 Pp.GA2 oxidase_5 SEQ ID NO: 584 SEQ ID NO: 585 Pp.GA2oxidase_6 SEQ ID NO: 586 SEQ ID NO: 587 Pp.GA2 oxidase_7 SEQ ID NO: 588SEQ ID NO: 589

A family of at least nine GA2 oxidase genes have been identified inbarrel clover (Medicago truncatula) including Mt.GA2 oxidase_1, Mt.GA2oxidase_2, Mt.GA2 oxidase_3, Mt.GA2 oxidase_4, Mt.GA2 oxidase_5, Mt.GA2oxidase_6, Mt.GA2 oxidase_7, Mt.GA2 oxidase_8, and Mt.GA2 oxidase_9. TheDNA and protein sequences by SEQ ID NO for each of these GA2 oxidasegenes from Medicago are provided in Table 14.

TABLE 14 DNA and protein sequences for GA2 oxidase genes in M.truncatula. Coding Sequence GA2 oxidase Gene (CDS) Protein Mt.GA2oxidase_1 SEQ ID NO: 590 SEQ ID NO: 591 Mt.GA2 oxidase_2 SEQ ID NO: 592SEQ ID NO: 593 Mt.GA2 oxidase_3 SEQ ID NO: 594 SEQ ID NO: 595 Mt.GA2oxidase_4 SEQ ID NO: 596 SEQ ID NO: 597 Mt.GA2 oxidase_5 SEQ ID NO: 598SEQ ID NO: 599 Mt.GA2 oxidase_6 SEQ ID NO: 600 SEQ ID NO: 601 Mt.GA2oxidase_7 SEQ ID NO: 602 SEQ ID NO: 603 Mt.GA2 oxidase_8 SEQ ID NO: 604SEQ ID NO: 605 Mt.GA2 oxidase_9 SEQ ID NO: 606 SEQ ID NO: 607

A family of at least four related GA2 oxidase genes have been identifiedin whorled honey flower (Paris polyphylla) including Ppo.GA2 oxidase_1,Ppo.GA2 oxidase_2, Ppo.GA2 oxidase_3, and Ppo.GA2 oxidase_4. The DNA andprotein sequences by SEQ ID NO for each of these GA2 oxidase genes fromhoney flower are provided in Table 15.

TABLE 15 DNA and protein sequences for GA2 oxidase genes in P.polyphylla. Coding Sequence GA2 oxidase Gene (CDS) Protein Ppo.GA2oxidase_1 SEQ ID NO: 608 SEQ ID NO: 609 Ppo.GA2 oxidase_2 SEQ ID NO: 610SEQ ID NO: 611 Ppo.GA2 oxidase_3 SEQ ID NO: 612 SEQ ID NO: 613 Ppo.GA2oxidase_4 SEQ ID NO: 614 SEQ ID NO: 615

A family of at least eight GA2 oxidase genes have been identified incommon bean (Phaseolus vulgaris) including Pv.GA2 oxidase_1, Pv.GA2oxidase_2, Pv.GA2 oxidase_3, Pv.GA2 oxidase_4, Pv.GA2 oxidase_5, Pv.GA2oxidase_6, Pv.GA2 oxidase_7, and Pv.GA2 oxidase_8. The DNA and proteinsequences by SEQ ID NO for each of these GA2 oxidase genes from commonbean are provided in Table 16.

TABLE 16 DNA and protein sequences for GA2 oxidase genes in common bean.Coding Sequence GA2 oxidase Gene (CDS) Protein Pv.GA2 oxidase_1 SEQ IDNO: 616 SEQ ID NO: 617 Pv.GA2 oxidase_2 SEQ ID NO: 618 SEQ ID NO: 619Pv.GA2 oxidase_3 SEQ ID NO: 620 SEQ ID NO: 621 Pv.GA2 oxidase_4 SEQ IDNO: 622 SEQ ID NO: 623 Pv.GA2 oxidase_5 SEQ ID NO: 624 SEQ ID NO: 625Pv.GA2 oxidase_6 SEQ ID NO: 626 SEQ ID NO: 627 Pv.GA2 oxidase_7 SEQ IDNO: 628 SEQ ID NO: 629 Pv.GA2 oxidase_8 SEQ ID NO: 630 SEQ ID NO: 631

A family of at least seven related GA2 oxidase genes have beenidentified in cottonwood (Populus trichocarpa) including Pt.GA2oxidase_1, Pt.GA2 oxidase_2, Pt.GA2 oxidase_3, Pt.GA2 oxidase_4, Pt.GA2oxidase_5, Pt.GA2 oxidase_6, and Pt.GA2 oxidase_7. The DNA and proteinsequences by SEQ ID NO for each of these GA2 oxidase genes fromcottonwood are provided in Table 17.

TABLE 17 DNA and protein sequences for GA2 oxidase genes in cottonwood.Coding Sequence GA2 oxidase Gene (CDS) Protein Pt.GA2 oxidase_1 SEQ IDNO: 632 SEQ ID NO: 633 Pt.GA2 oxidase_2 SEQ ID NO: 634 SEQ ID NO: 635Pt.GA2 oxidase_3 SEQ ID NO: 636 SEQ ID NO: 637 Pt.GA2 oxidase_4 SEQ IDNO: 638 SEQ ID NO: 639 Pt.GA2 oxidase_5 SEQ ID NO: 640 SEQ ID NO: 641Pt.GA2 oxidase_6 SEQ ID NO: 642 SEQ ID NO: 643 Pt.GA2 oxidase_7 SEQ IDNO: 644 SEQ ID NO: 645

A family of at least two GA2 oxidase genes have been identified in pea(Pisum sativum) including Ps.GA2 oxidase_1 and Ps.GA2 oxidase_2. The DNAand protein sequences by SEQ ID NO for these GA2 oxidase genes from peaare provided in Table 18.

TABLE 18 DNA and protein sequences for GA2 oxidase genes in pea. CodingSequence GA2 oxidase Gene (CDS) Protein Ps.GA2 oxidase_1 SEQ ID NO: 646SEQ ID NO: 647 Ps.GA2 oxidase_2 SEQ ID NO: 648 SEQ ID NO: 649

A family of at least three related GA2 oxidase genes have beenidentified in spinach (Spinacia oleracea) including So.GA2 oxidase_1,So.GA2 oxidase_2, and So.GA2 oxidase_3. The DNA and protein sequences bySEQ ID NO for each of these GA2 oxidase genes from spinach are providedin Table 19.

TABLE 19 DNA and protein sequences for GA2 oxidase genes in spinach.Coding Sequence GA2 oxidase Gene (CDS) Protein So.GA2 oxidase_1 SEQ IDNO: 650 SEQ ID NO: 651 So.GA2 oxidase_2 SEQ ID NO: 652 SEQ ID NO: 653So.GA2 oxidase_3 SEQ ID NO: 654 SEQ ID NO: 655

According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA construct is, orcomprises a sequence that is, at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 325, 327,329, 331, 333, 335, 337, 339, 341, 343, 345, 347, and/or 349. Accordingto some embodiments, a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 324, 326, 328, 330, 332, 334, 336, 338,340, 342, 344, 346, and/or 348.

According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 351, 353, 355, 357, 359, 361, 363, 365, 367 and/or 397. Accordingto some embodiments, a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 350, 352, 354, 356, 358, 360, 362, 364,366 and/or 368. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 371, 373, 375, 377, 379, 381, 383 and/or 385.According to some embodiments, a transcribable DNA sequence of arecombinant DNA molecule, vector or construct is or comprises a sequencethat is at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 370, 372, 374, 376, 378, 380,382 and/or 384. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 387, 389, 391, 393, 395, 397, 399, 401, 403, 405,407, 409, 411, 413, 415 and/or 417. According to some embodiments, atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410,412, 414 and/or 416. According to some embodiments, a GA2 oxidaseprotein encoded by a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 419, 421, 423, 425, 427, 429, 431, 433,435, 437, 439, 441, 443, 445 and/or 447. According to some embodiments,a transcribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442,444 and/or 446. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 449, 451, 453, 455, 457, 459, 461, 463, 465, 467and/or 469. According to some embodiments, a transcribable DNA sequenceof a recombinant DNA molecule, vector or construct is or comprises asequence that is at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%,or 100% identical to one or more of SEQ ID NOs: 448, 450, 452, 454, 456,458, 460, 462, 464, 466 and/or 468.

According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to a protein sequence froma dicot or leguminous plant. According to some embodiments, a GA2oxidase protein encoded by a transcribable DNA sequence of a recombinantDNA molecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 471, 473, 475, 477, 479, 481, 483, 485,487, 489, 491, 493, 495, 497, 499 and/or 501. According to someembodiments, a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 470, 472, 474, 476, 478, 480, 482, 484, 486, 488,490, 492, 494, 496, 498 and/or 500. According to some embodiments, a GA2oxidase protein encoded by a transcribable DNA sequence of a recombinantDNA molecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 503, 505, 507, 509, 511, 513, 515, 517,519, 521, 523, 525, 527, 529 and/or 531. According to some embodiments,a transcribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526,528 and/or 530. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 533, 535, 537, 539, 541, 543, 545, 547, 549, 551,553, 555, 557, 559 and/or 561. According to some embodiments, atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556,558 and/or 560. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 563, 565, 567, 569, 571, 573 and/or 575. Accordingto some embodiments, a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 562, 564, 566, 568, 570, 572 and/or 574.According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 577, 579, 581, 583, 585, 587 and/or 589. According to someembodiments, a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 576, 578, 580, 582, 584, 586 and/or 588. Accordingto some embodiments, a GA2 oxidase protein encoded by a transcribableDNA sequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 591, 593,595, 597, 599, 601, 603, 605 and/or 607. According to some embodiments,a transcribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 590, 592, 594, 596, 598, 600, 602, 604 and/or 606. According tosome embodiments, a GA2 oxidase protein encoded by a transcribable DNAsequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 609, 611,613 and/or 615. According to some embodiments, a transcribable DNAsequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 608, 610,612 and/or 614. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 617, 619, 621, 623, 625, 627, 629 and/or 631.According to some embodiments, a transcribable DNA sequence of arecombinant DNA molecule, vector or construct is or comprises a sequencethat is at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 616, 618, 620, 622, 624, 626,628 and/or 630. According to some embodiments, a GA2 oxidase proteinencoded by a transcribable DNA sequence of a recombinant DNA molecule,vector or construct is or comprises a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 633, 635, 637, 639, 641, 643 and/or 645. Accordingto some embodiments, a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 632, 634, 636, 638, 640, 642 and/or 644.According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 647 and/or 649. According to some embodiments, a transcribable DNAsequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 646 and/or648. According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 651, 653 and/or 655. According to some embodiments, a transcribableDNA sequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 650, 652and/or 654.

According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 325, 327, 329, 331, 333, 335, 337, 339, 341, 343, 345, 347, 349,351, 353, 355, 357, 359, 361, 363, 365, 367, 369, 371, 373, 375, 377,379, 381, 383, 385, 387, 389, 391, 393, 395, 397, 399, 401, 403, 405,407, 409, 411, 413, 415, 417, 419, 421, 423, 425, 427, 429, 431, 433,435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461,463, 465, 467 and/or 469. According to some embodiments, a transcribableDNA sequence of a recombinant DNA molecule, vector or construct is orcomprises a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 324, 326,328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354,356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382,384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410,412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438,440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466and/or 468.

According to some embodiments, a GA2 oxidase protein encoded by atranscribable DNA sequence of a recombinant DNA molecule, vector orconstruct is or comprises a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495,497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523,525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551,553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579,581, 583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607,609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635,637, 639, 641, 643, 645, 647, 649, 651, 653 and/or 655. According tosome embodiments, a transcribable DNA sequence of a recombinant DNAmolecule, vector or construct is or comprises a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 470, 472, 474, 476, 478, 480, 482, 484,486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512,514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540,542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568,570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596,598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624,626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652and/or 654.

According to embodiments of the present invention, the level(s) of oneor more active GAs may be reduced in the stalk or stem of a corn plantby ectopically expressing a catabolic GA2 oxidase gene to produce theshort stature phenotype and resistance to lodging in transgenic plants,but without off-types in the reproductive or ear tissues of the plant.

According to embodiments of the present invention, expression of a GA2oxidase transgene may be driven by a variety of differentplant-expressible promoter types including constitutive andtissue-specific or tissue-preferred promoters, such as a vascular orleaf promoter. According to present embodiments, a recombinant DNAmolecule, vector or construct for expression of a GA2 oxidase transgenein a plant is provided comprising a transcribable DNA sequence encodinga protein that is at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to a GA2 oxidase protein sequence providedherein, wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter, such as a constitutive, vascular or leafpromoter. According to some embodiments, a recombinant DNA molecule,vector or construct is provided comprising a transcribable DNA sequenceencoding a GA2 oxidase protein that comprises or consists of a sequencethat is at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 325, 327, 329, 331, 333, 335,337, 339, 341, 343, 345, 347 and/or 349, wherein the transcribable DNAsequence is operably linked to a plant-expressible promoter. Accordingto some of these embodiments, the transcribable DNA sequence of therecombinant DNA molecule, vector or construct comprises or consists of asequence that is at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%,or 100% identical to one or more of SEQ ID NOs: 324, 326, 328, 330, 332,334, 336, 338, 340, 342, 344, 346, and/or 348. According to someembodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, and/or348, wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some embodiments, thetranscribable DNA sequence encodes a GA2 oxidase protein. According tosome embodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 351, 353, 355, 357, 359, 361, 363, 365,367 and/or 369, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some of theseembodiments, the transcribable DNA sequence of the recombinant DNAmolecule, vector or construct comprises or consists of a sequence thatis at least 80%, at least 85%, at least 90%, at least 95%, at least 96%,at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 350, 352, 354, 356, 358, 360,362, 364, 366 and/or 368. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 350, 352, 354, 356, 358, 360, 362, 364, 366 and/or 368, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some embodiments, the transcribable DNA sequenceencodes a GA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 371, 373, 375, 377, 379, 381, 383, and/or385, wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some of these embodiments, thetranscribable DNA sequence of the recombinant DNA molecule, vector orconstruct comprises or consists of a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 370, 372, 374, 376, 378, 380, 382, and/or 384.According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 370, 372, 374, 376, 378, 380, 382, and/or 384, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some embodiments, the transcribable DNA sequenceencodes a GA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 387, 389, 391, 393, 395, 397, 399, 401,403, 405, 407, 409, 411, 413, 415, and/or 417, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some of these embodiments, the transcribable DNA sequenceof the recombinant DNA molecule, vector or construct comprises orconsists of a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 386, 388,390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, and/or416. According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410,412, 414, and/or 416, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 419, 421, 423, 425, 427, 429, 431, 433,435, 437, 439, 441, 443, 445, and/or 447, wherein the transcribable DNAsequence is operably linked to a plant-expressible promoter. Accordingto some of these embodiments, the transcribable DNA sequence of therecombinant DNA molecule, vector or construct comprises or consists of asequence that is at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%,or 100% identical to one or more of SEQ ID NOs: 418, 420, 421, 424, 426,428, 430, 432, 434, 436, 438, 440, 442, 444, and/or 446. According tosome embodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 418, 420, 421, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442,444, and/or 446, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 449, 451, 453, 455, 457, 459, 461, 463,465, 467, and/or 469, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some of theseembodiments, the transcribable DNA sequence of the recombinant DNAmolecule, vector or construct comprises or consists of a sequence thatis at least 80%, at least 85%, at least 90%, at least 95%, at least 96%,at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 448, 450, 452, 454, 456, 458,460, 462, 464, 466, and/or 468. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, and/or 468,wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some embodiments, thetranscribable DNA sequence encodes a GA2 oxidase protein. According tosome embodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 471, 473, 475, 477, 479, 481, 483, 485,487, 489, 491, 493, 495, 497, 499, and/or 501, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some of these embodiments, the transcribable DNA sequenceof the recombinant DNA molecule, vector or construct comprises orconsists of a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 470, 472,474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, and/or500. According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494,496, 498, and/or 500, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 503, 505, 507, 509, 511, 513, 515, 517,519, 521, 523, 525, 527, 529, and/or 531, wherein the transcribable DNAsequence is operably linked to a plant-expressible promoter. Accordingto some of these embodiments, the transcribable DNA sequence of therecombinant DNA molecule, vector or construct comprises or consists of asequence that is at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%,or 100% identical to one or more of SEQ ID NOs: 502, 504, 506, 508, 510,512, 514, 516, 518, 520, 522, 524, 526, 528, and/or 530. According tosome embodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526,528, and/or 530, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 533, 535, 537, 539, 541, 543, 545, 547,549, 551, 553, 555, 557, 559, and/or 561, wherein the transcribable DNAsequence is operably linked to a plant-expressible promoter. Accordingto some of these embodiments, the transcribable DNA sequence of therecombinant DNA molecule, vector or construct comprises or consists of asequence that is at least 80%, at least 85%, at least 90%, at least 95%,at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%,or 100% identical to one or more of SEQ ID NOs: 532, 534, 536, 538, 540,542, 544, 546, 548, 550, 552, 554, 556, 558, and/or 560. According tosome embodiments, the plant expressible promoter is a vascular promoter.According to some embodiments, the plant expressible promoter is avascular promoter comprising a sequence that is at least 70%, at least75%, at least 80%, at least 85%, at least 90%, at least 95%, at least96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or 100%identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660, 661,and/or 662, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a RTBV promoter(e.g., a promoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV(SEQ ID NO: 657) sequence) or a promoter comprising a sequence that isat least 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. Accordingto some embodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556,558, and/or 560, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 563, 565, 567, 569, 571, 573, and/or 575,wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some of these embodiments, thetranscribable DNA sequence of the recombinant DNA molecule, vector orconstruct comprises or consists of a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 562, 564, 566, 568, 570, 572, and/or 574. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 562, 564, 566, 568, 570, 572, and/or 574, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some embodiments, the transcribable DNA sequence encodes aGA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 577, 579, 581, 583, 585, 587, and/or 589,wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some of these embodiments, thetranscribable DNA sequence of the recombinant DNA molecule, vector orconstruct comprises or consists of a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 576, 578, 580, 582, 584, 586, and/or 588. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 576, 578, 580, 582, 584, 586, and/or 588, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some embodiments, the transcribable DNA sequence encodes aGA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 591, 593, 595, 597, 599, 601, 603, 605,and/or 607, wherein the transcribable DNA sequence is operably linked toa plant-expressible promoter. According to some of these embodiments,the transcribable DNA sequence of the recombinant DNA molecule, vectoror construct comprises or consists of a sequence that is at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 590, 592, 594, 596, 598, 600, 602, 604, and/or 608.According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 590, 592, 594, 596, 598, 600, 602, 604, and/or 608, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some embodiments, the transcribable DNA sequenceencodes a GA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 609, 611, 613, and/or 615, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some of these embodiments, the transcribable DNAsequence of the recombinant DNA molecule, vector or construct comprisesor consists of a sequence that is at least 80%, at least 85%, at least90%, at least 95%, at least 96%, at least 97%, at least 98%, at least99%, at least 99.5%, or 100% identical to one or more of SEQ ID NOs:608, 610, 612, and/or 614. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 608, 610, 612, and/or 614, wherein the transcribable DNA sequenceis operably linked to a plant-expressible promoter. According to someembodiments, the transcribable DNA sequence encodes a GA2 oxidaseprotein. According to some embodiments, the plant expressible promoteris a vascular promoter. According to some embodiments, the plantexpressible promoter is a vascular promoter comprising a sequence thatis at least 70%, at least \75%, at least 80%, at least 85%, at least90%, at least 95%, at least 96%, at least 97%, at least 98%, at least99%, at least 99.5%, or 100% identical to one or more of SEQ ID NOs:656, 657, 658, 659, 660, 661, and/or 662, or a functional portion of anyof the foregoing. According to some embodiments, the plant expressiblepromoter is a RTBV promoter (e.g., a promoter comprising the RTBV (SEQID NO: 656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 617, 619, 621, 623, 625, 627, 629, and/or631, wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some of these embodiments, thetranscribable DNA sequence of the recombinant DNA molecule, vector orconstruct comprises or consists of a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 616, 618, 620, 622, 624, 626, 628, and/or 630.According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 616, 618, 620, 622, 624, 626, 628, and/or 630, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some embodiments, the transcribable DNA sequenceencodes a GA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least \75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 633, 635, 637, 639, 641, 643, and/or 645,wherein the transcribable DNA sequence is operably linked to aplant-expressible promoter. According to some of these embodiments, thetranscribable DNA sequence of the recombinant DNA molecule, vector orconstruct comprises or consists of a sequence that is at least 80%, atleast 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 632, 634, 636, 638, 640, 642, and/or 644. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 632, 634, 636, 638, 640, 642, and/or 644, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some embodiments, the transcribable DNA sequence encodes aGA2 oxidase protein. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least \75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 647 and/or 649, wherein the transcribableDNA sequence is operably linked to a plant-expressible promoter.According to some of these embodiments, the transcribable DNA sequenceof the recombinant DNA molecule, vector or construct comprises orconsists of a sequence that is at least 80%, at least 85%, at least 90%,at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 646 and/or648. According to some embodiments, the plant expressible promoter is avascular promoter. According to some embodiments, the plant expressiblepromoter is a vascular promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to one or more of SEQ ID NOs: 656, 657, 658,659, 660, 661, and/or 662, or a functional portion of any of theforegoing. According to some embodiments, the plant expressible promoteris a RTBV promoter (e.g., a promoter comprising the RTBV (SEQ ID NO:656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 646 and/or 648, wherein the transcribable DNA sequence is operablylinked to a plant-expressible promoter. According to some embodiments,the transcribable DNA sequence encodes a GA2 oxidase protein. Accordingto some embodiments, the plant expressible promoter is a vascularpromoter. According to some embodiments, the plant expressible promoteris a vascular promoter comprising a sequence that is at least 70%, atleast \75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 656, 657, 658, 659, 660,661, and/or 662, or a functional portion of any of the foregoing.According to some embodiments, the plant expressible promoter is a RTBVpromoter (e.g., a promoter comprising the RTBV (SEQ ID NO: 656) ortruncated RTBV (SEQ ID NO: 657) sequence) or a promoter comprising asequence that is at least 70%, at least 75%, at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to SEQ ID NO: 656 and/or657. According to some embodiments, the plant expressible promoter is aleaf promoter. According to some embodiments, the plant expressiblepromoter is a leaf promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 663, 664, and/or 665, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a constitutive promoter.According to some embodiments, the plant expressible promoter is aconstitutive promoter comprising a sequence that is at least 70%, atleast 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence encoding aGA2 oxidase protein that comprises or consists of a sequence that is atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 651, 653, and/or 655, wherein thetranscribable DNA sequence is operably linked to a plant-expressiblepromoter. According to some of these embodiments, the transcribable DNAsequence of the recombinant DNA molecule, vector or construct comprisesor consists of a sequence that is at least 80%, at least 85%, at least90%, at least 95%, at least 96%, at least 97%, at least 98%, at least99%, at least 99.5%, or 100% identical to one or more of SEQ ID NOs:650, 652, and/or 654. According to some embodiments, the plantexpressible promoter is a vascular promoter. According to someembodiments, the plant expressible promoter is a vascular promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 656, 657, 658, 659, 660, 661, and/or 662, or afunctional portion of any of the foregoing. According to someembodiments, the plant expressible promoter is a RTBV promoter (e.g., apromoter comprising the RTBV (SEQ ID NO: 656) or truncated RTBV (SEQ IDNO: 657) sequence) or a promoter comprising a sequence that is at least70%, at least 75%, at least 80%, at least 85%, at least 90%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or 100% identical to SEQ ID NO: 656 and/or 657. According to someembodiments, the plant expressible promoter is a leaf promoter.According to some embodiments, the plant expressible promoter is a leafpromoter comprising a sequence that is at least 70%, at least 75%, atleast 80%, at least 85%, at least 90%, at least 95%, at least 96%, atleast 97%, at least 98%, at least 99%, at least 99.5%, or 100% identicalto one or more of SEQ ID NOs: 663, 664, and/or 665, or a functionalportion of any of the foregoing. According to some embodiments, theplant expressible promoter is a constitutive promoter. According to someembodiments, the plant expressible promoter is a constitutive promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to one ormore of SEQ ID NOs: 666, 667, 668, 669, 670, 671, 672, 673, and/or 674,or a functional portion of any of the foregoing.

According to some embodiments, a recombinant DNA molecule, vector orconstruct is provided comprising a transcribable DNA sequence thatcomprises or consists of a sequence that is at least 80%, at least 85%,at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, atleast 99%, at least 99.5%, or 100% identical to one or more of SEQ IDNOs: 650, 652, and/or 654, wherein the transcribable DNA sequence isoperably linked to a plant-expressible promoter. According to someembodiments, the transcribable DNA sequence encodes a GA2 oxidaseprotein. According to some embodiments, the plant expressible promoteris a vascular promoter. According to some embodiments, the plantexpressible promoter is a vascular promoter comprising a sequence thatis at least 70%, at least \75%, at least 80%, at least 85%, at least90%, at least 95%, at least 96%, at least 97%, at least 98%, at least99%, at least 99.5%, or 100% identical to one or more of SEQ ID NOs:656, 657, 658, 659, 660, 661, and/or 662, or a functional portion of anyof the foregoing. According to some embodiments, the plant expressiblepromoter is a RTBV promoter (e.g., a promoter comprising the RTBV (SEQID NO: 656) or truncated RTBV (SEQ ID NO: 657) sequence) or a promotercomprising a sequence that is at least 70%, at least 75%, at least 80%,at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, atleast 98%, at least 99%, at least 99.5%, or 100% identical to SEQ ID NO:656 and/or 657. According to some embodiments, the plant expressiblepromoter is a leaf promoter. According to some embodiments, the plantexpressible promoter is a leaf promoter comprising a sequence that is atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 95%, at least 96%, at least 97%, at least 98%, at least 99%, atleast 99.5%, or 100% identical to one or more of SEQ ID NOs: 663, 664,and/or 665, or a functional portion of any of the foregoing. Accordingto some embodiments, the plant expressible promoter is a constitutivepromoter. According to some embodiments, the plant expressible promoteris a constitutive promoter comprising a sequence that is at least 70%,at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, atleast 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, or100% identical to one or more of SEQ ID NOs: 666, 667, 668, 669, 670,671, 672, 673, and/or 674, or a functional portion of any of theforegoing.

According to many embodiments, a modified or transgenic corn plant isprovided comprising and/or transformed with a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinas provided herein. According to some embodiments, a modified ortransgenic corn plant is provided that is transformed with a recombinantDNA construct comprising a transcribable DNA sequence encoding a GA2oxidase mRNA and protein, wherein the transcribable DNA sequence isoperably linked to a plant-expressible promoter, wherein the GA2 oxidasemRNA and/or protein is identical to an endogenous GA2 oxidase protein,and wherein the expression level of the GA2 oxidase mRNA and/or proteinis increased in one or more plant tissue(s) of the modified ortransgenic plant as compared to a wild type or control plant, such asincreased in one or more vascular and/or leaf tissue(s) of the modifiedor transgenic plant, such as by at least 5%, at least 10%, at least 20%,at least 25%, at least 30%, at least 40%, at least 50%, at least 60%, atleast 70%, at least 75%, at least 80%, at least 90%, or 100%, ascompared to a wild type or control plant.

According to present embodiments, a modified or transgenic corn plant isprovided comprising a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein as providedherein, wherein the level of one or more active GAs, such as GA1, GA3,GA4, and/or GA7, is reduced or lowered in one or more plant tissue(s),such as one or more stem, internode, vascular and/or leaf tissue(s) orone or more stem and/or internode tissue(s), of the modified ortransgenic plant, such as by at least 5%, at least 10%, at least 20%, atleast 25%, at least 30%, at least 40%, at least 50%, at least 60%, atleast 70%, at least 75%, at least 80%, at least 90%, or 100%, ascompared to a wild type or control plant.

According to many embodiments, a modified or transgenic plant isprovided that is transformed with a recombinant DNA construct comprisinga transcribable DNA sequence encoding a GA2 oxidase protein as providedherein, wherein the transcribable DNA sequence is operably linked to aconstitutive promoter or a tissue-specific or tissue-preferred promoter,such as a vascular promoter or a leaf promoter, and wherein the modifiedor transgenic plant has one or more of the following traits: asemi-dwarf or reduced plant height or stature, decreased stem internodelength, increased lodging resistance, and/or increased stem or stalkdiameter. Such a modified or transgenic plant may not have anysignificant reproductive off-types. A modified or transgenic plant mayhave one or more of the following additional traits: reduced green snap,deeper roots, increased leaf area, earlier canopy closure, higherstomatal conductance, lower ear height, increased foliar water content,improved drought tolerance, increased nitrogen use efficiency, increasedwater use efficiency, reduced anthocyanin content and anthocyanin areain leaves under normal and/or nitrogen or water limiting stressconditions, increased ear weight, increased kernel number, increasedkernel weight, increased yield, and/or increased harvest index.According to many embodiments, the level of one or more active GAs, suchas GA1, GA3, GA4, and/or GA7, is/are reduced or lowered in one or moreplant tissue(s), such as one or more stem, internode, vascular and/orleaf tissue(s), or one or more stem and/or internode tissue(s), of themodified or transgenic plant, such as by at least 5%, at least 10%, atleast 20%, at least 25%, at least 30%, at least 40%, at least 50%, atleast 60%, at least 70%, at least 75%, at least 80%, at least 90%, or100%, as compared to a wild type or control plant.

A recombinant DNA molecule, construct or vector of the presentdisclosure may comprise a transcribable DNA sequence encoding a GA2oxidase as provided herein, wherein the transcribable DNA sequence isoperatively linked to a plant-expressible promoter, such as aconstitutive or vascular and/or leaf promoter. In addition to itsassociated promoter, a transcribable DNA sequence encoding a GA2 oxidasemay also be operatively linked to one or more additional regulatoryelement(s), such as an enhancer(s), leader, transcription start site(TSS), linker, 5′ and 3′ untranslated region(s) (UTRs), intron(s),polyadenylation signal, termination region or sequence, etc., that aresuitable, necessary or preferred for strengthening, regulating orallowing expression of the transcribable DNA sequence in a corn plantcell. Such additional regulatory element(s) may be optional and/or usedto enhance or optimize expression of the transgene or transcribable DNAsequence. As provided herein, an “enhancer” may be distinguished from a“promoter” in that an enhancer typically lacks a transcription startsite, TATA box, or equivalent sequence and is thus insufficient alone todrive transcription. As used herein, a “leader” may be defined generallyas the DNA sequence of the 5′-UTR of a gene (or transgene) between thetranscription start site (TSS) and 5′ end of the transcribable DNAsequence or protein coding sequence start site of the transgene.

Transgenic plants expressing a GA2 oxidase transgene may have an earliercanopy closure (e.g., approximately one day earlier, or 12-48 hours,12-36 hours, 18-36 hours, or about 24 hours earlier canopy closure) thana wild type or control plant. Although transgenic plants expressing aGA2 oxidase transgene may have a lower ear height than a wild type orcontrol plant, the height of the ear may generally be at least 18 inchesabove the ground. Transgenic plants expressing a GA2 oxidase may havegreater biomass and/or leaf area during one or more late vegetativestages (e.g., V8-V12) than a wild type or control plant. Transgenicplants expressing a GA2 oxidase may have deeper roots during latervegetative stages when grown in the field, than a wild type or controlplant, which may be due to an increased root front velocity. Thesetransgenic plants may reach a depth 90 cm below ground sooner (e.g.,5-25 days sooner, 5-20 days sooner, 5-15 days sooner, 10-25 days sooner,or 15-25 days sooner, or about 5, 10, 15, 20 Or 25 days sooner) than awild type or control plant, which may occur by or prior to thevegetative to reproductive transition of the plant (e.g., by V16/R1 atabout 50 days after planting as opposed to about 70 days after plantingfor control plants).

According to some embodiments, a recombinant DNA construct or vector maycomprise two or more expression elements or cassettes that may bestacked together in a construct or vector either in tandem in a singleexpression cassette or separately in two or more expression cassettes. Arecombinant DNA construct or vector may comprise either a singleexpression cassette comprising a transcribable DNA sequence that encodesa GA2 oxidase mRNA and protein or two or more expression cassettescomprising two or more transcribable DNA sequences that encode two ormore GA2 oxidase mRNAs and proteins, including at least a first GA2oxidase mRNA and protein and a second GA2 oxidase mRNA and protein,wherein the two or more transcribable DNA sequences, GA2 oxidase mRNAsand/or GA2 oxidase proteins are the same or different, and wherein eachtranscribable DNA sequence is operably linked to a plant-expressiblepromoter. The plant-expressible promoter may be a constitutive promoter,or a tissue-specific or tissue-preferred promoter, as provided herein.If two or more transcribable DNA sequences are present in a recombinantDNA construct or vector or a modified or transgenic plant, plant part,cell, or explant, each transcribable DNA sequence may be operably linkedto the same or different plant-expressible promoters.

According to other embodiments, a recombinant DNA construct or vectormay comprise two or more expression cassettes including a firstexpression cassette and a second expression cassette, wherein the firstexpression cassette comprises a first transcribable DNA sequenceoperably linked to a first plant-expressible promoter, and the secondexpression cassette comprises a second transcribable DNA sequenceoperably linked to a second plant-expressible promoter, wherein thefirst transcribable DNA sequence encodes a first GA2 oxidase and thesecond transcribable DNA sequence encodes a second GA2 oxidase. Thefirst and second plant-expressible promoters may each be a constitutivepromoter, or a tissue-specific or tissue-preferred promoter, as providedherein, and the first and second plant-expressible promoters may be thesame or different promoters.

According to other embodiments, two or more constructs, expressioncassettes or transgenes encoding one or more GA2 oxidase proteins may becombined in a modified plant by crossing two or more plants together inone or more generations to produce a modified plant having a desiredcombination of the constructs, expression cassettes or transgenes.According to these embodiments, a first modified plant comprising afirst construct, expression cassette or transgene encoding a first GA2oxidase protein may be crossed to a second modified plant comprising asecond construct, expression cassette or transgene encoding a second GA2oxidase protein, such that a modified progeny plant may be madecomprising the first construct, expression cassette or transgene and thesecond construct, expression cassette or transgene. Alternatively, amodified plant comprising two or more constructs, expression cassettesor transgenes encoding two or more GA2 oxidase proteins may be made by(i) co-transforming a first construct, expression cassette or transgeneand a second construct, expression cassette or transgene (each encodinga GA2 oxidase protein) in the same or different transformation moleculesor vectors, (ii) transforming a modified plant with a second construct,expression cassette or transgene in a transformation molecule or vector,wherein the modified plant already comprises a first construct,expression cassette or transgene, or (iii) transforming a plant with afirst construct, expression cassette or transgene in a firsttransformation molecule or vector, and then transforming the plant witha second construct, expression cassette or transgene in a secondtransformation molecule or vector.

According to embodiments of the present disclosure, modified plants areprovided comprising two or more constructs comprising GA2 oxidasetransgene(s) including a first recombinant DNA construct and a secondrecombinant DNA construct, wherein the first recombinant DNA constructcomprises a first transcribable DNA sequence encoding a first GA2oxidase mRNA and protein, and the second recombinant DNA constructcomprises a second transcribable DNA sequence encoding a second GA2oxidase mRNA and protein. The first and second recombinant DNAconstructs may be stacked in a single vector and transformed into aplant as a single event, or present in separate vectors or constructsthat may be transformed as separate events. According to someembodiments, the first and second GA2 oxidase transgenes may be the sameor different GA oxidase gene(s).

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter fertilization or silking of at least 50% of said plurality of cornplants, wherein fewer than or equal to 50% of said corn plants havelodged at the time of harvest, and wherein at least one corn plant ofsaid plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter at least 50% of said corn plants have reached R3 stage, whereinfewer than or equal to 50% of said corn plants have lodged at the timeof harvest, and wherein at least one corn plant of said plurality ofcorn plants comprises a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter fertilization or silking of at least 50% of said plurality of cornplants, wherein the average kernel moisture content is less than orequal to 30%, and wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter at least 50% of said corn plants have reached R3 stage, whereinaverage kernel moisture content is less than or equal to 30%, andwherein fewer than or equal to 50% of said corn plants have lodged atthe time of harvest, and wherein at least one corn plant of saidplurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter at fertilization or silking of at least 50% of said plurality ofcorn plants, wherein the average yield of said field is at least 170bushels per acre, wherein fewer than or equal to 50% of said corn plantshave lodged at the time of harvest, and wherein at least one corn plantof said plurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 50 daysafter at least 50% of said corn plants have reached R3 stage, whereinthe average yield of said field is at least 170 bushels per acre,wherein fewer than or equal to 50% of said corn plants have lodged atthe time of harvest, and wherein at least one corn plant of saidplurality of corn plants comprises a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.

In some embodiments, this disclosure provides a method comprisingharvesting a plurality of corn plants from a field at least 1 day afterthe average kernel moisture content of at least 50% of said plurality ofcorn plants is between 10% and 30%, wherein fewer than or equal to 50%of said corn plants have lodged at the time of harvest, and wherein atleast one corn plant of said plurality of corn plants comprises arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter.

In an aspect, at least 10% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, at least 20% of the cornplants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, atleast 30% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, at least 40% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, at least 50% of the cornplants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, atleast 60% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, at least 70% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, at least 80% of the cornplants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, atleast 90% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, 100% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter.

In an aspect, between 1% and 100% of the corn plants in a field comprisea recombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, between 10% and 100% of thecorn plants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, between20% and 100% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, between 30% and 100% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, between 40% and 100% of thecorn plants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, between50% and 100% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, between 60% and 100% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter. In an aspect, between 70% and 100% of thecorn plants in a field comprise a recombinant DNA construct comprising atranscribable DNA sequence encoding a GA2 oxidase protein and aplant-expressible promoter, wherein the transcribable DNA sequence isoperably linked to the plant-expressible promoter. In an aspect, between80% and 100% of the corn plants in a field comprise a recombinant DNAconstruct comprising a transcribable DNA sequence encoding a GA2 oxidaseprotein and a plant-expressible promoter, wherein the transcribable DNAsequence is operably linked to the plant-expressible promoter. In anaspect, between 90% and 100% of the corn plants in a field comprise arecombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter.

A corn field is considered to be “harvested” when at least one ear hasbeen removed from most, all, or a majority of the corn plants in thefield. A corn plant(s) is/are considered to be “harvested” when at leastone ear has been removed from the corn plant(s), or at least from mostor a majority of the corn plants.

As used herein, a “field” refers to an outdoor location that is suitablefor growing corn, and a “corn field” refers to a field that has beenplanted with a plurality or population of corn plants. The field orlocation can be irrigated or non-irrigated. A corn field can comprise aland area planted with corn seed and/or at least one corn plant or aplurality of corn plants, which can be at one or more stages ofdevelopment. According to some aspects, a plurality of corn plants in afield can be at a homogeneous or the same (or nearly homogeneous ornearly the same) stage of development, such that the plurality of cornplants have approximately the same height. In an aspect, a corn plantprovided herein is planted in a field.

In another aspect, a corn plant provided herein is not planted in thefield, but is planted indoors, such as in a greenhouse, and/or in acontainer holding a growth medium or soil.

A corn field can comprise one or more rows of corn plants of the same ordifferent lengths. As used herein, a “row” comprises a plurality of cornplants in a linear or near linear arrangement. In an aspect, a rowcomprises at least two corn plants. Without being limiting, a row ofcorn plants is planted in a line, and if a corn field comprises two ormore rows, they are typically planted parallel to each other. A cornfield can comprise one or more rows of corn plants where the rows are ofthe same or different lengths. Without being limiting, a corn fieldcomprises at least 1 row of corn plants. In another aspect, a corn fieldcomprises at least 10 rows of corn plants. In another aspect, a cornfield comprises at least 50 rows of corn plants. In another aspect, acorn field comprises at least 500 rows of corn plants. In anotheraspect, a corn field comprises at least 1,000 rows of corn plants. Inanother aspect, a corn field comprises at least 5,000 rows of cornplants. In another aspect, a corn field comprises at least 10,000 rowsof corn plants.

In an aspect, a corn field comprises rows that are spaced at least 5inches apart. In another aspect, a corn field comprises rows that arespaced at least 10 inches apart. In a further aspect, a corn fieldcomprises rows that are spaced at least 15 inches apart. In an aspect, acorn field comprises rows of corn plants that are spaced at least 20inches apart. In another aspect, a corn field comprises rows of cornthat are spaced at least 25 inches apart. In another aspect, a cornfield comprises rows of corn that are spaced at least 30 inches apart.According to some aspects, a corn field can comprise two or morepluralities of corn plants with the pluralities of corn plants beingplanted with different corn varieties, at different times, at differentdensities, in different arrangements (e.g., in rows or scattered orrandom placement), and/or at different row spacings and/or row lengths,such that the pluralities of corn plants have different heights,spacings, etc., at different time points during the growing season,although each plurality of corn plants can be relatively uniform withrespect to plant height and other growth metrics.

In an aspect, a field comprises a single plot. In another aspect, afield comprises multiple plots. In another aspect, one or more edges ofa field are bordered by a fence. In another aspect, one or more edges ofa field are unfenced. In another aspect, one or more edges of a fieldare bordered by hedges. In an aspect, a field comprises a physicallycontiguous space. In another aspect, the field comprises a physicallynon-contiguous space. In still another aspect, the field comprises abiologically contiguous space. As used herein, a “biologicallycontiguous space” refers to a space where the pollen can move from onesection of a field to another. In an aspect, a biologically contiguousfield is physically contiguous. In another aspect, a biologicallycontiguous field is physically non-contiguous (e.g., plots within thefield or a single plot within the field can be separated by a structure,without being limiting, such as a road, creek, irrigation ditch, trail,hedgerow, fence, irrigation pipes, fallow field, empty field, ornon-corn plants).

In an aspect, a field comprises at least 0.5 acres. In an aspect, afield comprises at least 1 acre. In another aspect, a field comprises atleast 5 acres. In another aspect, a field comprises at least 10 acres.In another aspect, a field comprises at least 15 acres. In anotheraspect, a field comprises at least 20 acres. In another aspect, a fieldcomprises at least 25 acres. In another aspect, a field comprises atleast 30 acres. In another aspect, a field comprises at least 35 acres.In another aspect, a field comprises at least 40 acres. In anotheraspect, a field comprises at least 45 acres. In another aspect, a fieldcomprises at least 50 acres. In another aspect, a field comprises atleast 75 acres. In another aspect, a field comprises at least 100 acres.In another aspect, a field comprises at least 150 acres. In anotheraspect, a field comprises at least 200 acres. In another aspect, a fieldcomprises at least 250 acres. In another aspect, a field comprises atleast 300 acres. In another aspect, a field comprises at least 350acres. In another aspect, a field comprises at least 400 acres. Inanother aspect, a field comprises at least 450 acres. In another aspect,a field comprises at least 500 acres. In another aspect, a fieldcomprises at least 750 acres. In another aspect, a field comprises atleast 1000 acres. In another aspect, a field comprises at least 1500acres. In another aspect, a field comprises at least 2000 acres. Inanother aspect, a field comprises at least 2500 acres. In anotheraspect, a field comprises at least 3000 acres. In another aspect, afield comprises at least 4000 acres. In another aspect, a fieldcomprises at least 5000 acres. In another aspect, a field comprises atleast 10,000 acres.

In an aspect, a field comprises between 0.5 acres and 10,000 acres. Inanother aspect, a field comprises between 1 acre and 10,000 acres. Inanother aspect, a field comprises between 5 acres and 10,000 acres. Inanother aspect, a field comprises between 10 acres and 10,000 acres. Inanother aspect, a field comprises between 15 acres and 10,000 acres. Inanother aspect, a field comprises between 20 acres and 10,000 acres. Inanother aspect, a field comprises between 25 acres and 10,000 acres. Inanother aspect, a field comprises between 30 acres and 10,000 acres. Inanother aspect, a field comprises between 35 acres and 10,000 acres. Inanother aspect, a field comprises between 40 acres and 10,000 acres. Inanother aspect, a field comprises between 45 acres and 10,000 acres. Inanother aspect, a field comprises between 50 acres and 10,000 acres. Inanother aspect, a field comprises between 75 acres and 10,000 acres. Inanother aspect, a field comprises between 100 acres and 10,000 acres. Inanother aspect, a field comprises between 150 acres and 10,000 acres. Inanother aspect, a field comprises between 200 acres and 10,000 acres. Inanother aspect, a field comprises between 250 acres and 10,000 acres. Inanother aspect, a field comprises between 300 acres and 10,000 acres. Inanother aspect, a field comprises between 350 acres and 10,000 acres. Inanother aspect, a field comprises between 400 acres and 10,000 acres. Inanother aspect, a field comprises between 450 acres and 10,000 acres. Inanother aspect, a field comprises between 500 acres and 10,000 acres. Inanother aspect, a field comprises between 750 acres and 10,000 acres. Inanother aspect, a field comprises between 1000 acres and 10,000 acres.In another aspect, a field comprises between 1500 acres and 10,000acres. In another aspect, a field comprises between 2000 acres and10,000 acres. In another aspect, a field comprises between 2500 acresand 10,000 acres. In another aspect, a field comprises between 3000acres and 10,000 acres. In another aspect, a field comprises between4000 acres and 10,000 acres. In another aspect, a field comprisesbetween 5000 acres and 10,000 acres. In another aspect, a fieldcomprises between 1 acre and 5000 acres. In another aspect, a fieldcomprises between 1 acre and 2500 acres. In another aspect, a fieldcomprises between 1 acre and 1000 acres. In another aspect, a fieldcomprises between 1 acre and 500 acres. In another aspect, a fieldcomprises between 1 acre and 250 acres. In another aspect, a fieldcomprises between 1 acre and 100 acres. In another aspect, a fieldcomprises between 1 acre and 75 acres. In another aspect, a fieldcomprises between 1 acre and 50 acres. In another aspect, a fieldcomprises between 1 acre and 25 acres. In another aspect, a fieldcomprises between 1 acre and 10 acres.

In an aspect, a corn field can further comprise plants other than cornplants including, without being limiting, cotton, alfalfa, sunflowers,sorghum, wheat, barley, oat, rice, rye, soybean, vegetables (e.g.,potato, tomato, carrot), grass (e.g., bluegrass, Triticale), and weeds.

In an aspect, a corn field comprises a density of at least 10,000 cornplants per acre. In another aspect, a corn field comprises a density ofat least 15,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 20,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 22,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 24,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 26,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 28,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 30,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 32,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 34,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 36,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 38,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 40,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 42,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 44,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 46,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 48,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 50,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 52,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 54,000 corn plants per acre. In another aspect, a corn fieldcomprises a density of at least 56,000 corn plants per acre. In anotheraspect, a corn field comprises a density of at least 58,000 corn plantsper acre. In another aspect, a corn field comprises a density of atleast 60,000 corn plants per acre.

In an aspect, a corn field comprises a density of between 10,000 and50,000 corn plants per acre. In an aspect, a corn field comprises adensity of between 10,000 and 40,000 corn plants per acre. In an aspect,a corn field comprises a density of between 10,000 and 30,000 cornplants per acre. In an aspect, a corn field comprises a density ofbetween 10,000 and 25,000 corn plants per acre. In an aspect, a cornfield comprises a density of between 10,000 and 20,000 corn plants peracre. In an aspect, a corn field comprises a density of between 20,000corn plants and 60,000 corn plants per acre. In an aspect, a corn fieldcomprises a density of between 20,000 corn plants and 58,000 corn plantsper acre. In an aspect, a corn field comprises a density of between20,000 corn plants and 55,000 corn plants per acre. In an aspect, a cornfield comprises a density of between 20,000 corn plants and 50,000 cornplants per acre. In an aspect, a corn field comprises a density ofbetween 20,000 corn plants and 45,000 corn plants per acre. In anaspect, a corn field comprises a density of between 20,000 corn plantsand 42,000 corn plants per acre. In an aspect, a corn field comprises adensity of between 20,000 corn plants and 40,000 corn plants per acre.In an aspect, a corn field comprises a density of between 20,000 cornplants and 38,000 corn plants per acre. In an aspect, a corn fieldcomprises a density of between 20,000 corn plants and 36,000 corn plantsper acre. In an aspect, a corn field comprises a density of between20,000 corn plants and 34,000 corn plants per acre. In an aspect, a cornfield comprises a density of between 20,000 corn plants and 32,000 cornplants per acre. In an aspect, a corn field comprises a density ofbetween 20,000 corn plants and 30,000 corn plants per acre. In anaspect, a corn field comprises a density of between 24,000 corn plantsand 58,000 corn plants per acre. In an aspect, a corn field comprises adensity of between 38,000 corn plants and 60,000 corn plants per acre.In an aspect, a corn field comprises a density of between 38,000 cornplants and 50,000 corn plants per acre.

In an aspect, a corn field comprises at least 10 corn plants. In anotheraspect, a corn field comprises at least 10 corn plants per acre. In anaspect, a corn field comprises at least 100 corn plants. In anotheraspect, a corn field comprises at least 100 corn plants per acre. In anaspect, a corn field comprises at least 500 corn plants. In anotheraspect, a corn field comprises at least 500 corn plants per acre. In anaspect, a corn field comprises at least 1000 corn plants. In anotheraspect, a corn field comprises at least 1000 corn plants per acre. In anaspect, a corn field comprises at least 5000 corn plants. In anotheraspect, a corn field comprises at least 5000 corn plants per acre. In anaspect, a corn field comprises at least 10,000 corn plants. In anaspect, a corn field comprises at least 10,000 corn plants per acre. Inan aspect, a corn field comprises at least 12,000 corn plants. In anaspect, a corn field comprises at least 12,000 corn plants per acre. Inan aspect, a corn field comprises at least 15,000 corn plants. In anaspect, a corn field comprises at least 15,000 corn plants per acre. Inan aspect, a corn field comprises at least 18,000 corn plants. In anaspect, a corn field comprises at least 18,000 corn plants per acre. Inan aspect, a corn field comprises at least 20,000 corn plants. In anaspect, a corn field comprises at least 20,000 corn plants per acre. Inan aspect, a corn field comprises at least 22,000 corn plants. In anaspect, a corn field comprises at least 22,000 corn plants per acre. Inan aspect, a corn field comprises at least 24,000 corn plants. In anaspect, a corn field comprises at least 24,000 corn plants per acre. Inan aspect, a corn field comprises at least 26,000 corn plants. In anaspect, a corn field comprises at least 26,000 corn plants per acre. Inan aspect, a corn field comprises at least 28,000 corn plants. In anaspect, a corn field comprises at least 28,000 corn plants per acre. Inan aspect, a corn field comprises at least 30,000 corn plants. In anaspect, a corn field comprises at least 30,000 corn plants per acre. Inan aspect, a corn field comprises at least 32,000 corn plants. In anaspect, a corn field comprises at least 32,000 corn plants per acre. Inan aspect, a corn field comprises at least 34,000 corn plants. In anaspect, a corn field comprises at least 34,000 corn plants per acre. Inan aspect, a corn field comprises at least 36,000 corn plants. In anaspect, a corn field comprises at least 36,000 corn plants per acre. Inan aspect, a corn field comprises at least 38,000 corn plants. In anaspect, a corn field comprises at least 38,000 corn plants per acre. Inan aspect, a corn field comprises at least 40,000 corn plants. In anaspect, a corn field comprises at least 40,000 corn plants per acre. Inan aspect, a corn field comprises at least 42,000 corn plants. In anaspect, a corn field comprises at least 42,000 corn plants per acre. Inan aspect, a corn field comprises at least 44,000 corn plants. In anaspect, a corn field comprises at least 44,000 corn plants per acre. Inan aspect, a corn field comprises at least 46,000 corn plants. In anaspect, a corn field comprises at least 46,000 corn plants per acre. Inan aspect, a corn field comprises at least 48,000 corn plants. In anaspect, a corn field comprises at least 48,000 corn plants per acre. Inan aspect, a corn field comprises at least 50,000 corn plants. In anaspect, a corn field comprises at least 50,000 corn plants per acre. Inan aspect, a corn field comprises at least 52,000 corn plants. In anaspect, a corn field comprises at least 52,000 corn plants per acre. Inan aspect, a corn field comprises at least 54,000 corn plants. In anaspect, a corn field comprises at least 54,000 corn plants per acre. Inan aspect, a corn field comprises at least 56,000 corn plants. In anaspect, a corn field comprises at least 56,000 corn plants per acre. Inan aspect, a corn field comprises at least 58,000 corn plants. In anaspect, a corn field comprises at least 58,000 corn plants per acre. Inan aspect, a corn field comprises at least 60,000 corn plants. In anaspect, a corn field comprises at least 60,000 corn plants per acre.

In an aspect, a corn field comprises between 10,000 corn plants per acreand 50,000 corn plants per acre. In an aspect, a corn field comprisesbetween 10,000 corn plants per acre and 40,000 corn plants per acre. Inan aspect, a corn field comprises between 10,000 corn plants per acreand 30,000 corn plants per acre. In an aspect, a corn field comprisesbetween 10,000 corn plants per acre and 25,000 corn plants per acre. Inan aspect, a corn field comprises between 10,000 corn plants per acreand 20,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 60,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 58,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 55,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 50,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 45,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 42,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 40,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 38,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 36,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 34,000 corn plants per acre. In an aspect, a corn field comprisesbetween 20,000 corn plants per acre and 32,000 corn plants per acre. Inan aspect, a corn field comprises between 20,000 corn plants per acreand 30,000 corn plants per acre. In an aspect, a corn field comprisesbetween 24,000 corn plants per acre and 58,000 corn plants per acre. Inan aspect, a corn field comprises between 38,000 corn plants per acreand 60,000 corn plants per acre. In an aspect, a corn field comprisesbetween 38,000 corn plants per acre and 50,000 corn plants per acre.

In an aspect, a corn field comprises at least 0.5 acres. In anotheraspect, a corn field comprises at least 1 acre. In another aspect, acorn field comprises at least 3 acres. In another aspect, a corn fieldcomprises at least 5 acres. In another aspect, a corn field comprises atleast 10 acres. In another aspect, a corn field comprises at least 15acres. In another aspect, a corn field comprises at least 20 acres. Inanother aspect, a corn field comprises at least 25 acres. In anotheraspect, a corn field comprises at least 50 acres. In another aspect, acorn field comprises at least 75 acres. In another aspect, a corn fieldcomprises at least 100 acres. In another aspect, a corn field comprisesat least 150 acres. In another aspect, a corn field comprises at least200 acres. In another aspect, a corn field comprises at least 250 acres.In another aspect, a corn field comprises at least 300 acres. In anotheraspect, a corn field comprises at least 350 acres. In another aspect, acorn field comprises at least 400 acres. In another aspect, a corn fieldcomprises at least 500 acres. In another aspect, a corn field comprisesat least 750 acres. In another aspect, a corn field comprises at least1000 acres. In another aspect, a corn field comprises at least 2500acres. In another aspect, a corn field comprises at least 5 acres. Inanother aspect, a corn field comprises at least 5000 acres.

In an aspect, a corn field comprises between 0.5 acres and 5000 acres.In another aspect, a corn field comprises between 1 acre and 5000 acres.In another aspect, a corn field comprises between 5 acres and 5000acres. In another aspect, a corn field comprises between 10 acres and5000 acres. In another aspect, a corn field comprises between 25 acresand 5000 acres. In another aspect, a corn field comprises between 50acres and 5000 acres. In another aspect, a corn field comprises between100 acres and 5000 acres. In another aspect, a corn field comprisesbetween 200 acres and 5000 acres. In another aspect, a corn fieldcomprises between 500 acres and 5000 acres. In another aspect, a cornfield comprises between 1000 acres and 5000 acres. In another aspect, acorn field comprises between 1 acre and 500 acres. In another aspect, acorn field comprises between 1 acre and 400 acres. In another aspect, acorn field comprises between 1 acre and 300 acres. In another aspect, acorn field comprises between 1 acre and 250 acres. In another aspect, acorn field comprises between 1 acre and 200 acres. In another aspect, acorn field comprises between 1 acre and 150 acres. In another aspect, acorn field comprises between 1 acre and 100 acres. In another aspect, acorn field comprises between 1 acre and 75 acres. In another aspect, acorn field comprises between 1 acre and 50 acres. In another aspect, acorn field comprises between 1 acre and 25 acres. In another aspect, acorn field comprises between 10 acres and 25 acres. In another aspect, acorn field comprises between 10 acres and 50 acres. In another aspect, acorn field comprises between 10 acres and 100 acres. In another aspect,a corn field comprises between 10 acres and 250 acres. In anotheraspect, a corn field comprises between 10 acres and 500 acres. Inanother aspect, a corn field comprises between 100 acres and 250 acres.In another aspect, a corn field comprises between 100 acres and 500acres.

As used herein, the term “yield” refers to the amount of harvested plantmaterial or grain, such as kernels or seeds, but may also or insteadinclude the amount of biomass harvested (including for example, stalk,leaves, and/or kernels), from the plant(s). Harvested grain can be usedin a variety of applications including food processing, animal feed,etc., and biomass may be used for a variety of applications includingsileage, biofuel, etc., as known in the art. In an aspect, yield ismeasured as the amount of biomass or sileage harvested from theplant(s). In another aspect, yield is measured in bushels per acre. Inanother aspect, yield is measured in average number of kernels per ear.In still another aspect, yield is measured in grams per dry kernel. Instill another aspect, yield is measured in terms of average kernelweight and the average number of kernels per ear. In still anotheraspect, yield is measured in Standard Seed Units (SSU) per acre. One SSUfor corn is equivalent to 80,000 corn seed kernels. The number ofStandard Seed Units (SSUs) is appropriate for seed production since itquantifies the number of plants that can potentially be grown from thequantity of seeds, whereas yield takes into account both seed number andseed size.

In an aspect, the average yield of a corn field comprises at least 100bushels per acre. In an aspect, the average yield of a corn fieldcomprises at least 120 bushels per acre. In an aspect, the average yieldof a corn field comprises at least 130 bushels per acre. In an aspect,the average yield of a corn field comprises at least 140 bushels peracre. In an aspect, the average yield of a corn field comprises at least150 bushels per acre. In an aspect, the average yield of a corn fieldcomprises at least 160 bushels per acre. In an aspect, the average yieldof a corn field comprises at least 170 bushels per acre. In an aspect,the average yield of a corn field comprises at least 180 bushels peracre. In an aspect, the average yield of a corn field comprises at least190 bushels per acre. In an aspect, the average yield of a corn fieldcomprises at least 200 bushels per acre. In an aspect, the average yieldof a corn field comprises at least 210 bushels per acre. In an aspect,the average yield of a corn field comprises at least 220 bushels peracre. In an aspect, the average yield of a corn field comprises at least230 bushels per acre. In an aspect, the average yield of a corn fieldcomprises at least 240 bushels per acre. In an aspect, the average yieldof a corn field comprises at least 250 bushels per acre. In an aspect,the average yield of a corn field comprises at least 260 bushels peracre.

In an aspect, the average yield of a corn field comprises between 100bushels per acre and 260 bushels per acre. In an aspect, the averageyield of a corn field comprises between 120 bushels per acre and 260bushels per acre. In an aspect, the average yield of a corn fieldcomprises between 140 bushels per acre and 260 bushels per acre. In anaspect, the average yield of a corn field comprises between 160 bushelsper acre and 260 bushels per acre. In an aspect, the average yield of acorn field comprises between 180 bushels per acre and 260 bushels peracre. In an aspect, the average yield of a corn field comprises between200 bushels per acre and 260 bushels per acre. In an aspect, the averageyield of a corn field comprises between 220 bushels per acre and 260bushels per acre. In an aspect, the average yield of a corn fieldcomprises between 240 bushels per acre and 260 bushels per acre. In anaspect, the average yield of a corn field comprises between 100 bushelsper acre and 200 bushels per acre. In an aspect, the average yield of acorn field comprises between 150 bushels per acre and 250 bushels peracre. In an aspect, the average yield of a corn field comprises between150 bushels per acre and 200 bushels per acre.

In an aspect, the average yield of a corn field harvested at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days at, least 90 days, at least 100 days, or at least110 days after fertilization or silking is within 5% of the averageyield of a corn field comprising plants of the same genetic backgroundharvested between 20 and 30 days after fertilization or silking. In anaspect, the average yield of a corn field harvested at least 30 days, atleast 40 days, at least 50 days, at least 60 days, at least 70 days, atleast 80 days at, least 90 days, at least 100 days, or at least 110 daysafter fertilization or silking is within 10% of the average yield of acorn field comprising plants of the same genetic background harvestedbetween 20 and 30 days after fertilization or silking. In an aspect, theaverage yield of a corn field harvested at least 30 days, at least 40days, at least 50 days, at least 60 days, at least 70 days, at least 80days, at least 90 days, at least 100 days, or at least 110 days afterfertilization or silking is within 15% of the average yield of a cornfield comprising plants of the same genetic background harvested between20 and 30 days after fertilization or silking. In an aspect, the averageyield of a corn field harvested at least 30 days, at least 40 days, atleast 50 days, at least 60 days, at least 70 days, at least 80 days, atleast 90 days, at least 100 days, or at least 110 days afterfertilization or silking is within 20% of the average yield of a cornfield comprising plants of the same genetic background harvested between20 and 30 days after fertilization or silking. In an aspect, the averageyield of a corn field comprising plants of the same genetic backgroundharvested at least 30 days, at least 40 days, at least 50 days, at least60 days, at least 70 days, at least 80 days, at least 90 days, at least100 days, or at least 110 days after fertilization or silking is within25% of the average yield of a corn field comprising plants of the samegenetic background harvested between 20 and 30 days after fertilizationor silking. In an aspect, the average yield of a corn field comprisingplants of the same genetic background harvested at least 30 days, atleast 40 days, at least 50 days, at least 60 days, at least 70 days, atleast 80 days, at least 90 days, at least 100 days, or at least 110 daysafter fertilization or silking is within 30% of the average yield of acorn field comprising plants of the same genetic background harvestedbetween 20 and 30 days after fertilization or silking.

Kernel moisture content can be measured by any means typically used inthe art. Non-limiting examples for measuring kernel moisture contentinclude the use of an electronic grain moisture tester (e.g., infraredmonitors); direct measurement of water content via a chemical reaction(e.g., the Karl Fischer method); and drying whole kernel samples andmeasuring weight loss during drying.

In an aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of less than or equal to30%. In an aspect, a method provided herein comprises harvesting cornplants comprising an average kernel moisture content of less than orequal to 29%. In an aspect, a method provided herein comprisesharvesting corn plants comprising an average kernel moisture content ofless than or equal to 28%. In an aspect, a method provided hereincomprises harvesting corn plants comprising an average kernel moisturecontent of less than or equal to 27%. In an aspect, a method providedherein comprises harvesting corn plants comprising an average kernelmoisture content of less than or equal to 26%. In an aspect, a methodprovided herein comprises harvesting corn plants comprising an averagekernel moisture content of less than or equal to 25%. In an aspect, amethod provided herein comprises harvesting corn plants comprising anaverage kernel moisture content of less than or equal to 24%. In anaspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of less than or equal to23%. In an aspect, a method provided herein comprises harvesting cornplants comprising an average kernel moisture content of less than orequal to 22%. In an aspect, a method provided herein comprisesharvesting corn plants comprising an average kernel moisture content ofless than or equal to 21%. In an aspect, a method provided hereincomprises harvesting corn plants comprising an average kernel moisturecontent of less than or equal to 20%. In an aspect, a method providedherein comprises harvesting corn plants comprising an average kernelmoisture content of less than or equal to 19%. In an aspect, a methodprovided herein comprises harvesting corn plants comprising an averagekernel moisture content of less than or equal to 18%. In an aspect, amethod provided herein comprises harvesting corn plants comprising anaverage kernel moisture content of less than or equal to 17%. In anaspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of less than or equal to16%. In an aspect, a method provided herein comprises harvesting cornplants comprising an average kernel moisture content of less than orequal to 15%. In an aspect, a method provided herein comprisesharvesting corn plants comprising an average kernel moisture content ofless than or equal to 14%. In an aspect, a method provided hereincomprises harvesting corn plants comprising an average kernel moisturecontent of less than or equal to 13%. In an aspect, a method providedherein comprises harvesting corn plants comprising an average kernelmoisture content of less than or equal to 12%. In an aspect, a methodprovided herein comprises harvesting corn plants comprising an averagekernel moisture content of less than or equal to 10%. Each of the aboveaverage kernel moisture content ranges may also apply to the kernelmoisture content of a corn plant, such as the kernel moisture content ofa corn plant of a plurality of corn plants.

In an aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 10% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 11% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 12% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 13% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 14% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 15% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 16% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 17% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 18% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 19% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 20% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 21% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 22% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 23% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 24% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 25% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 26% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 27% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 28% and 30%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 29% and 30%.Each of the above average kernel moisture content ranges may also applyto the kernel moisture content of a corn plant of the plurality of cornplants.

In an aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 10% and 25%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 10% and 20%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 10% and 15%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 10% and 13%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 13% and 25%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 13% and 20%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 13% and 15%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 15% and 25%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 15% and 20%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average kernel moisture content of between 20% and 25%.Each of the above average kernel moisture content ranges may also applyto the kernel moisture content of a corn plant of the plurality of cornplants.

In an aspect, methods are provided comprising harvesting a plurality ofcorn plants in a field at least 1 day, at least 2 days, at least 3 days,at least 4 days, at least 5 days, at least 6 days, at least 7 days, atleast 8 days, at least 9 days, at least 10 days, at least 11 days, atleast 12 days, at least 13 days, at least 14 days, at least 15 days, atleast 20 days, at least 25 days, at least 30 days, at least 35 days, atleast 40 days, at least 45 days, at least 50 days, at least 55 days, atleast 60 days, at least 65 days, at least 70 days, at least 75 days, atleast 80 days, at least 90 days, at least 95 days, at least 100 days, orat least 1 week, at least 2 weeks, at least 3 weeks, at least 4 weeks,at least 5 weeks, at least 6 weeks, at least 7 weeks, at least 8 weeks,at least 9 weeks, at least 10 weeks, at least 11 weeks, at least 12weeks, at least 13 weeks, at least 14 weeks, or at least 15 weeks aftera desired or acceptable kernel moisture content or average kernelmoisture content is reached (without being limiting, for example,between 10% and 30%, between 10% and 25%, between 13% and 25%, between15% and 25%, between 10% and 20%, or between 15% and 30% or any otherspecific moisture content percentage within any of such moisture contentranges or as provided herein), which may comprise measuring the moisturecontent of one or more ears or kernels of a corn plant of the pluralityof corn plants in the field (or an average kernel moisture content fortwo or more corn plants of the plurality of corn plants), and harvestingthe plurality of corn plants if the desired or acceptable kernelmoisture content or desired or acceptable average kernel moisturecontent is reached.

As used herein, an “acceptable” or “desired” kernel moisture content canbe any specific kernel moisture content percentage, or any kernelmoisture content within a range of kernel moisture content percentages,provided herein. An “average kernel moisture content” for a plurality ofcorn plants is the average moisture content of kernels from two or morecorn plants, such as from two or more corn plants of a plurality of cornplants. An “average kernel moisture content” for a single corn plant isthe average moisture content of two or more kernels from a corn plant.

Leaf moisture content or stalk moisture content can also be measuredusing techniques standard in the art.

In an aspect, a method provided herein comprises harvesting corn plantscomprising an average stalk moisture content and/or an average leafmoisture content of equal to or less than 30%. In an aspect, a methodprovided herein comprises harvesting corn plants comprising an averagestalk moisture content and/or an average leaf moisture content of equalto or less than 25%. In an aspect, a method provided herein comprisesharvesting corn plants comprising an average stalk moisture contentand/or an average leaf moisture content of equal to or less than 20%. Inan aspect, a method provided herein comprises harvesting corn plantscomprising an average stalk moisture content and/or an average leafmoisture content of equal to or less than 15%. In an aspect, a methodprovided herein comprises harvesting corn plants comprising an averagestalk moisture content and/or an average leaf moisture content of equalto or less than 10%. Each of the above average stalk and/or leafmoisture content ranges may also apply to the stalk or leaf moisturecontent of a corn plant of the plurality of corn plants.

In another aspect, a method provided herein comprises harvesting cornplants comprising an average stalk moisture content and/or an averageleaf moisture content of between 10% and 30%. In another aspect, amethod provided herein comprises harvesting corn plants comprising anaverage stalk moisture content and/or an average leaf moisture contentof between 15% and 30%. In another aspect, a method provided hereincomprises harvesting corn plants comprising an average stalk moisturecontent and/or an average leaf moisture content of between 15% and 25%or a stalk or leaf moisture content of a corn plant, such as a cornplant of a plurality of corn plants, of between 15% and 25%.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field where fewer than or equal to 95% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where fewer than or equal to 90% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where fewer than orequal to 80% of the corn plants have lodged at the time of harvest. Inan aspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field where fewer than or equal to 70% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where fewer than or equal to 60% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where fewer than orequal to 50% of the corn plants have lodged at the time of harvest. Inan aspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field where fewer than or equal to 45% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where fewer than or equal to 40% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where fewer than orequal to 35% of the corn plants have lodged at the time of harvest. Inan aspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field where fewer than or equal to 30% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where fewer than or equal to 25% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where fewer than orequal to 20% of the corn plants have lodged at the time of harvest. Inan aspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field where fewer than or equal to 15% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where fewer than or equal to 10% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where fewer than orequal to 5% of the corn plants have lodged at the time of harvest. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a field where fewer than or equal to 1% of the cornplants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where 0% of the corn plants have lodged at the time of harvest.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a field where between 0% and 100% of the corn plantshave lodged at the time of harvest. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a fieldwhere between 0% and 90% of the corn plants have lodged at the time ofharvest. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a field where between 0% and 80% of thecorn plants have lodged at the time of harvest. In an aspect, a methodprovided herein comprises harvesting a plurality of corn plants from afield where between 0% and 70% of the corn plants have lodged at thetime of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 0% and60% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 0% and 50% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 0% and40% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 0% and 30% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 0% and25% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 0% and 20% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 0% and15% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 0% and 10% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 10% and50% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 10% and 20% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 10% and30% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 20% and 50% of the corn plants have lodged atthe time of harvest. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a field where between 30% and50% of the corn plants have lodged at the time of harvest. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a field where between 40% and 50% of the corn plants have lodged atthe time of harvest.

The height of a corn plant can be determined based on a variety ofanatomical locations on a corn plant. In an aspect, the height of a cornplant is measured as the distance between the top of the soil or groundand the ligule or collar of the uppermost fully-expanded leaf of a cornplant. As used herein, a “fully-expanded leaf” is a leaf where the leafblade is exposed, and both the ligule and auricle are visible at theblade/sheath boundary. In another aspect, the height of a corn plant ismeasured as the distance between the top of the soil or ground and theupper leaf surface of the leaf farthest from the soil or ground. In afurther aspect, the height of a corn plant is measured as the distancebetween the top of the soil or ground and the arch of the highest cornleaf that is at least 50% developed. In still a further aspect, theheight of a corn plant is measured as the distance between the top ofthe soil or ground and the anatomical part of the corn plant that isfarthest from the top of the soil or ground. Exemplary, non-limitingmethods of measuring plant height include comparing photographs of cornplants to a height reference, or physically measuring individual cornplants with a suitable ruler. If not otherwise stated, the height of acorn plant for the present disclosure is measured as the distancebetween the top of the soil or ground and the collar of the uppermostfully-expanded leaf of a corn plant. If not otherwise stated, alldescriptions herein with regard to the plant height of a population ofplants can refer to either the average plant height among the populationof plants or, if stated, the percentage(s) of plants among thepopulation of plants.

Short stature corn plants typically have improved standability andreduced lodging as compared to taller corn plants. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.9 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.8 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.7 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.6 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.5 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.4 meters at the time of harvest. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.3 meters at the time of harvest.

In an aspect, the average height of corn plants in a corn field providedherein is less than or equal to 1.9 meters at R1 stage, R2 stage, R3stage, R4 stage, or R5 stage or later. In an aspect, the average heightof corn plants in a corn field provided herein is less than or equal to1.8 meters at R1 stage, R2 stage, R3 stage, R4 stage, or R5 stage orlater. In an aspect, the average height of corn plants in a corn fieldprovided herein is less than or equal to 1.7 meters at R1 stage, R2stage, R3 stage, R4 stage, or R5 stage or later. In an aspect, theaverage height of corn plants in a corn field provided herein is lessthan or equal to 1.6 meters at R1 stage, R2 stage, R3 stage, R4 stage,or R5 stage or later. In an aspect, the average height of corn plants ina corn field provided herein is less than or equal to 1.5 meters at R1stage, R2 stage, R3 stage, R4 stage, or R5 stage or later. In an aspect,the average height of corn plants in a corn field provided herein isless than or equal to 1.4 meters at R1 stage, R2 stage, R3 stage, R4stage, or R5 stage or later. In an aspect, the average height of cornplants in a corn field provided herein is less than or equal to 1.3meters at R1 stage, R2 stage, R3 stage, R4 stage, or R5 stage or later.In an aspect, the average height of corn plants in a corn field providedherein is less than or equal to 1.2 meters at R1 stage, R2 stage, R3stage, R4 stage, or R5 stage or later. In an aspect, the average heightof corn plants in a corn field provided herein is less than or equal to1.1 meters at R1 stage, R2 stage, R3 stage, R4 stage, or R5 stage orlater.

In an aspect, the average height of corn plants in a corn field providedherein is between 1.1 meters and 1.9 meters at the time of harvest. Inan aspect, the average height of corn plants in a corn field providedherein is between 1.3 meters and 1.8 meters at the time of harvest. Inan aspect, the average height of corn plants in a corn field providedherein is between 1.3 meters and 1.7 meters at the time of harvest. Inan aspect, the average height of corn plants in a corn field providedherein is between 1.4 meters and 1.7 meters at the time of harvest. Inan aspect, the average height of corn plants in a corn field providedherein is between 1.5 meters and 1.7 meters at the time of harvest. Inan aspect, the average height of corn plants in a corn field providedherein is between 1.6 meters and 1.7 meters at the time of harvest.

Corn leaves consist of four main anatomical parts: a proximal sheath, aligule, an auricle, and a distal blade. The sheath wraps around the stemand younger leaves, while the blade is flattened in the mediolateralaxis (midrib to margin). The ligule and auricle are found at theblade/sheath boundary; the ligule is an adaxial (upper) membranousstructure that acts as a collar around the stem, and the auricle is aprojection on the lower surface of the blade base that connects theblade to the sheath. Stages of corn plant growth are divided intovegetative (V) stages and reproductive (R) stages. Upon germination, acorn plant is said to be in VE stage (emergence). Once the first leafcollar (e.g., the ligule) is visible, the corn plant is in the V1 stage.The emergence of the second leaf collar signifies V2 stage; theemergence of the third leaf collar signifies the V3 stage; and so onuntil the tassel emerges. For example, if twelve leaf collars arevisible, the plant is a V12 stage plant. Once the bottom-most branch ofthe tassel emerges the plant is in VT stage, which is the finalvegetative stage. The reproductive stage of growth occurs after thevegetative stage. The number of vegetative stages prior to VT stage canvary by environment and corn line. The first reproductive stage (R1;silking stage; “silking”) occurs when silk is visible outside the huskleaves surrounding an ear of corn. R2 (blistering stage) occurs whencorn kernels are white on the outside and are filled with a clear liquidinside. R3 (milk stage) occurs when the kernels are yellow on theoutside and are filled with a milky white fluid inside. R4 (dough stage)occurs when the kernels are filled with a thick, or pasty, fluid. Insome corn lines the cob will also turn pink or red at this stage. R5(dent stage) occurs when a majority of the kernels are at leastpartially dented. The final reproductive stage, R6 (physiologicalmaturity), occurs when the kernels have attained their maximum dryweight.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field at least 20 days after at least 50% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 30 days after at least 50% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 40 days after at least 50% of corn plants in the corn fieldhave reached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, at least 100 days, or at least110 days after at least 50%, at least 60%, at least 70%, at least 80%,at least 90% or 100% of corn plants in the corn field have reached R3stage. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 55 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field at least 60 days after at least 50% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 75 days after at least 50% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 90 days after at least 50% of corn plants in the corn fieldhave reached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 120days after at least 50% of corn plants in the corn field have reached R3stage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field between 20 days and 120 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 30 days and 120 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 40 days and 120 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 120 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 90 days after at least50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 80 days after at least50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 70 days after at least50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 120 days after atleast 50% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 90 days after at least50% of corn plants in the corn field have reached R3 stage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field at least 20 days after at least 75% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 30 days after at least 75% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 40 days after at least 75% of corn plants in the corn fieldhave reached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, at least 100 days, or at least110 days after at least 75% of corn plants in the corn field havereached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 55 daysafter at least 75% of corn plants in the corn field have reached R3stage. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 60 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field at least 75 days after at least 75% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 90 days after at least 75% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 120 days after at least 75% of corn plants in the corn fieldhave reached R3 stage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field between 20 days and 120 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 30 days and 120 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 40 days and 120 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 120 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 90 days after at least75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 80 days after at least75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 70 days after at least75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 120 days after atleast 75% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 90 days after at least75% of corn plants in the corn field have reached R3 stage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field at least 20 days after at least 90% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 30 days after at least 90% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 40 days after at least 90% of corn plants in the corn fieldhave reached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 30days, at least 40 days, at least 50 days, at least 60 days, at least 70days, at least 80 days, at least 90 days, at least 100 days, or at least110 days after at least 90% of corn plants in the corn field havereached R3 stage. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 55 daysafter at least 90% of corn plants in the corn field have reached R3stage. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 60 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field at least 75 days after at least 90% ofcorn plants in the corn field have reached R3 stage. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 90 days after at least 90% of corn plants inthe corn field have reached R3 stage. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 120 days after at least 90% of corn plants in the corn fieldhave reached R3 stage.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field between 20 days and 120 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 30 days and 120 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 40 days and 120 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 120 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 90 days after at least90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 80 days after at least90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 70 days after at least90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 120 days after atleast 90% of corn plants in the corn field have reached R3 stage. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 90 days after at least90% of corn plants in the corn field have reached R3 stage.

As used herein, the term “fertilization” refers to the union of a malegamete and a female gamete to produce a kernel, or fertilized egg,following pollination. In an aspect, fertilization is performed by wind.In another aspect, fertilization is performed by human intervention. Inanother aspect, fertilization is performed by an animal or insect.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field at least 30 days after fertilization orsilking of the plurality of corn plants. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 35 days after fertilization or silking of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 40 days afterfertilization or silking of the plurality of corn plants. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 45 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 50 days after fertilization or silking of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 55 days afterfertilization or silking of the plurality of corn plants. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 60 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 65 days after fertilization or silking of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 70 days afterfertilization or silking of the plurality of corn plants. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 75 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 80 days after fertilization or silking of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 85 days afterfertilization or silking of the plurality of corn plants. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 90 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 120 days after fertilization or silking of the plurality of cornplants.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field between 50 days and 120 days afterfertilization or silking of the plurality of corn plants. In an aspect,a method provided herein comprises harvesting a plurality of corn plantsfrom a corn field between 50 days and 90 days after fertilization orsilking of the plurality of corn plants. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldbetween 50 days and 80 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn fieldbetween 50 days and 70 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn fieldbetween 60 days and 120 days after fertilization or silking of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn fieldbetween 60 days and 90 days after fertilization or silking of theplurality of corn plants.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field at least 30 days after fertilization orsilking of at least 50%, at least 60%, at least 70%, at least 80%, atleast 90%, or 100% of the plurality of corn plants. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 35 days after fertilization or silking of atleast 50%, at least 60%, at least 70%, at least 80%, at least 90%, or100% of the plurality of corn plants. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 40 days after fertilization or silking of at least 50%, atleast 60%, at least 70%, at least 80%, at least 90%, or 100% of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 45 days after fertilization or silking of at least 50%, at least60%, at least 70%, at least 80%, at least 90%, or 100% of the pluralityof corn plants. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 50 daysafter fertilization or silking of at least 50%, at least 60%, at least70%, at least 80%, at least 90%, or 100% of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 55 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field at least 60 days after fertilization orsilking of at least 50%, at least 60%, at least 70%, at least 80%, atleast 90%, or 100% of the plurality of corn plants. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 65 days after fertilization or silking of atleast 50%, at least 60%, at least 70%, at least 80%, at least 90%, or100% of the plurality of corn plants. In an aspect, a method providedherein comprises harvesting a plurality of corn plants from a corn fieldat least 70 days after fertilization or silking of at least 50%, atleast 60%, at least 70%, at least 80%, at least 90%, or 100% of theplurality of corn plants. In an aspect, a method provided hereincomprises harvesting a plurality of corn plants from a corn field atleast 75 days after fertilization or silking of at least 50%, at least60%, at least 70%, at least 80%, at least 90%, or 100% of the pluralityof corn plants. In an aspect, a method provided herein comprisesharvesting a plurality of corn plants from a corn field at least 80 daysafter fertilization or silking of at least 50%, at least 60%, at least70%, at least 80%, at least 90%, or 100% of the plurality of cornplants. In an aspect, a method provided herein comprises harvesting aplurality of corn plants from a corn field at least 85 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field at least 90 days after fertilization orsilking of at least 50%, at least 60%, at least 70%, at least 80%, atleast 90%, or 100% of the plurality of corn plants. In an aspect, amethod provided herein comprises harvesting a plurality of corn plantsfrom a corn field at least 120 days after fertilization or silking of atleast 50%, at least 60%, at least 70%, at least 80%, at least 90%, or100% of the plurality of corn plants.

In an aspect, a method provided herein comprises harvesting a pluralityof corn plants from a corn field between 50 days and 120 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 90 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 80 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 50 days and 70 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 120 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants. In anaspect, a method provided herein comprises harvesting a plurality ofcorn plants from a corn field between 60 days and 90 days afterfertilization or silking of at least 50%, at least 60%, at least 70%, atleast 80%, at least 90%, or 100% of the plurality of corn plants.

The following non-limiting embodiments are specifically envisioned:

-   -   1. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_3 locus, wherein the mutant allele        comprises a DNA segment inserted into the endogenous GA20        oxidase_3 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_3 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   2. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a DNA segment inserted into the endogenous GA20        oxidase_5 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_5 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   3. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of an        endogenous Brachytic2 (br2) locus, wherein the mutant allele        comprises a DNA segment inserted into the endogenous br2 locus,        wherein the DNA segment encodes an antisense RNA that is at        least 70% complementary to at least 20 consecutive nucleotides        of SEQ ID NO: 132 or 180, and wherein the mutant allele of the        endogenous br2 locus produces an RNA transcript comprising the        antisense RNA sequence.    -   4. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of an        endogenous Brachytic2 (br2) locus, wherein the mutant allele        comprises a deletion of at least one nucleotide from an        endogenous br2 locus as compared to SEQ ID NO: 132.    -   5. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a dominant or semi-dominant        transgene or mutant allele of a gene, and wherein the transgene        or mutant allele causes a short stature phenotype in the at        least one corn plant.    -   6. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a premature stop codon within        a nucleic acid sequence encoding a Brachytic2 protein as        compared to a control corn plant.    -   7. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genome modification comprising a deletion of at        least a portion of the transcription termination sequence of the        endogenous Zm.SAMT gene, and wherein the mutant allele produces        a RNA molecule comprising an antisense sequence complementary to        all or part of the sense strand of the endogenous GA20 oxidase_5        gene.    -   8. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genome modification comprising a deletion of at        least a portion of the intergenic region between the endogenous        GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutant allele        produces a RNA molecule comprising an antisense sequence        complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   9. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genome modification comprising a deletion of at        least a portion of one or more of the following: 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′        UTR, and any portion thereof, and the 5′ UTR, 1^(st) exon,        1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd)        intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th) intron,        6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th)        exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMT        gene.    -   10. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genome modification which results in the        transcription of an antisense strand of at least an exon, an        intron, or an untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof    -   11. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises the Zm.SAMT gene promoter, or a functional part        thereof, operably linked to at least one transcribable antisense        sequence of at least an exon, intron or untranslated region        (UTR) of the endogenous GA20 oxidase_5 gene, or any portion        thereof    -   12. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a sequence selected from the group consisting of SEQ        ID NOs: 87-105.    -   13. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a first sequence and a second sequence; wherein the        first sequence comprises one or more of the 5′ UTR, 1^(st) exon,        1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR,        and any complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any complementary sequence thereof, and        any portion of the foregoing, of the endogenous Zm.SAMT gene;        wherein the first sequence and the second sequence are        contiguous or separated only by an intervening sequence of fewer        than 555 nucleotides.    -   14. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genomic deletion relative to a wild type allele of        the endogenous GA20 oxidase_5 locus, wherein the genomic        deletion is flanked by a first sequence and a second sequence;        wherein the first sequence comprises one or more of the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3′ UTR, and any complementary sequence thereof, and any        portion of the foregoing, of the endogenous Zm.GA20 oxidase_5        gene; and wherein the second sequence comprises one or more of        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron,        5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)        exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any complementary        sequence thereof, and any portion of the foregoing, of the        endogenous Zm.SAMT gene.    -   15. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a mutant allele of the        endogenous GA20 oxidase_5 locus, wherein the mutant allele        comprises a genomic sequence comprising a first sequence and a        second sequence; wherein the first sequence comprises at least        15 consecutive nucleotides of one or more of SEQ ID NOs: 228-235        and 276-283; wherein the second sequence comprises at least 15        consecutive nucleotides of one or more of SEQ ID NOs: 235-276;        and wherein the genomic sequence is at least 50 consecutive        nucleotides in length, and/or fewer than 9000 consecutive        nucleotides in length.    -   16. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_3        locus, wherein the mutant allele comprises a DNA segment        inserted into the endogenous GA20 oxidase_3 locus, wherein the        DNA segment encodes an antisense RNA sequence that is at least        70% complementary to at least 20 consecutive nucleotides of one        or more of SEQ ID NOs: 182-184 and 186-188, and wherein the        mutant allele of the endogenous GA20 oxidase_3 locus produces a        RNA transcript comprising the antisense RNA sequence.    -   17. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a DNA segment        inserted into the endogenous GA20 oxidase_5 locus, wherein the        DNA segment encodes an antisense RNA sequence that is at least        70% complementary to at least 20 consecutive nucleotides of one        or more of SEQ ID NOs: 182-184 and 186-188, and wherein the        mutant allele of the endogenous GA20 oxidase_5 locus produces a        RNA transcript comprising the antisense RNA sequence.    -   18. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of an endogenous Brachytic2 (br2)        locus, wherein the mutant allele comprises a DNA segment        inserted into the endogenous br2 locus, wherein the DNA segment        encodes an antisense RNA that is at least 70% complementary to        at least 20 consecutive nucleotides of SEQ ID NO: 132 or 180,        and wherein the mutant allele of the endogenous br2 locus        produces an RNA transcript comprising the antisense RNA        sequence.    -   19. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of an endogenous Brachytic2 (br2)        locus, wherein the mutant allele comprises a deletion of at        least one nucleotide from an endogenous br2 locus as compared to        SEQ ID NO: 132.    -   20. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a dominant or semi-dominant transgene or mutant allele        of a gene, and wherein the transgene or mutant allele causes a        short stature phenotype in the at least one corn plant.    -   21. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a premature stop codon within a nucleic acid sequence        encoding a Brachytic2 protein as compared to a control corn        plant.    -   22. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genome modification        comprising a deletion of at least a portion of the transcription        termination sequence of the endogenous Zm.SAMT gene, and wherein        the mutant allele produces a RNA molecule comprising an        antisense sequence complementary to all or part of the sense        strand of the endogenous GA20 oxidase_5 gene.    -   23. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genome modification        comprising a deletion of at least a portion of the intergenic        region between the endogenous GA20 oxidase_5 and Zm.SAMT genes,        and wherein the mutant allele produces a RNA molecule comprising        an antisense sequence complementary to all or part of the sense        strand of the endogenous GA20 oxidase_5 gene.    -   24. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genome modification        comprising a deletion of at least a portion of one or more of        the following: 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon,        2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion thereof, and        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron,        5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)        exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any portion        thereof, of the endogenous Zm.SAMT gene.    -   25. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genome modification        which results in the transcription of an antisense strand of at        least an exon, an intron, or an untranslated region (UTR) of the        endogenous GA20 oxidase_5 gene, or any portion thereof    -   26. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises the Zm.SAMT gene        promoter, or a functional part thereof, operably linked to at        least one transcribable antisense sequence of at least an exon,        intron or untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof.    -   27. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a sequence selected        from the group consisting of SEQ ID NOs: 87-105.    -   28. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a first sequence and        a second sequence; wherein the first sequence comprises one or        more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon,        2^(nd) intron, 3^(rd) exon, 3′ UTR, and any complementary        sequence thereof, and any portion of the foregoing, of the        endogenous Zm.GA20 oxidase_5 gene; and wherein the second        sequence comprises one or more of the 5′ UTR, 1^(st) exon,        1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd)        intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th) intron,        6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th)        exon, 3′ UTR, and any complementary sequence thereof, and any        portion of the foregoing, of the endogenous Zm.SAMT gene;        wherein the first sequence and the second sequence are        contiguous or separated only by an intervening sequence of fewer        than 555 nucleotides.    -   29. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genomic deletion        relative to a wild type allele of the endogenous GA20 oxidase_5        locus, wherein the genomic deletion is flanked by a first        sequence and a second sequence; wherein the first sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any        complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) i_(n)t_(ron), 3^(rd)        exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,        5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th)        intron, 8^(th) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.SAMT gene.    -   30. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a mutant allele of the endogenous GA20 oxidase_5        locus, wherein the mutant allele comprises a genomic sequence        comprising a first sequence and a second sequence; wherein the        first sequence comprises at least 15 consecutive nucleotides of        one or more of SEQ ID NOs: 228-235 and 276-283; wherein the        second sequence comprises at least 15 consecutive nucleotides of        one or more of SEQ ID NOs: 235-276; and wherein the genomic        sequence is at least 50 consecutive nucleotides in length,        and/or fewer than 9000 consecutive nucleotides in length.    -   31. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_3 locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous GA20        oxidase_3 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_3 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   32. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous GA20        oxidase_5 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_5 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   33. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of an endogenous Brachytic2 (br2) locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous br2        locus, wherein the DNA segment encodes an antisense RNA that is        at least 70% complementary to at least 20 consecutive        nucleotides of SEQ ID NO: 132 or 180, and wherein the mutant        allele of the endogenous br2 locus produces an RNA transcript        comprising the antisense RNA sequence.    -   34. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of an endogenous Brachytic2 (br2) locus, wherein the mutant        allele comprises a deletion of at least one nucleotide from an        endogenous br2 locus as compared to SEQ ID NO: 132.    -   35. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a dominant or        semi-dominant transgene or mutant allele of a gene, and wherein        the transgene or mutant allele causes a short stature phenotype        in the at least one corn plant.    -   36. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a premature        stop codon within a nucleic acid sequence encoding a Brachytic2        protein as compared to a control corn plant.    -   37. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of the transcription termination sequence of        the endogenous Zm.SAMT gene, and wherein the mutant allele        produces a RNA molecule comprising an antisense sequence        complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   38. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of the intergenic region between the        endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the        mutant allele produces a RNA molecule comprising an antisense        sequence complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   39. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of one or more of the following: 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any portion thereof, of the endogenous        Zm.SAMT gene.    -   40. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification which results in the        transcription of an antisense strand of at least an exon, an        intron, or an untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof    -   41. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises the Zm.SAMT gene promoter, or a functional part        thereof, operably linked to at least one transcribable antisense        sequence of at least an exon, intron or untranslated region        (UTR) of the endogenous GA20 oxidase_5 gene, or any portion        thereof.    -   42. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a sequence selected from the group consisting        of SEQ ID NOs: 87-105.    -   43. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a first sequence and a second sequence; wherein        the first sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′        UTR, and any complementary sequence thereof, and any portion of        the foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and        wherein the second sequence comprises one or more of the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,        5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th)        intron, 8^(th) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.SAMT gene; wherein the first sequence and the second sequence        are contiguous or separated only by an intervening sequence of        fewer than 555 nucleotides.    -   44. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genomic deletion relative to a wild type        allele of the endogenous GA20 oxidase_5 locus, wherein the        genomic deletion is flanked by a first sequence and a second        sequence; wherein the first sequence comprises one or more of        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.GA20 oxidase_5 gene; and wherein the second sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)        exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon,        6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR,        and any complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.SAMT gene.    -   45. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genomic sequence comprising a first sequence        and a second sequence; wherein the first sequence comprises at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        228-235 and 276-283; wherein the second sequence comprises at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        235-276; and wherein the genomic sequence is at least 50        consecutive nucleotides in length, and/or fewer than 9000        consecutive nucleotides in length.    -   46. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_3 locus, wherein the mutant allele comprises a DNA        segment inserted into the endogenous GA20 oxidase_3 locus,        wherein the DNA segment encodes an antisense RNA sequence that        is at least 70% complementary to at least 20 consecutive        nucleotides of one or more of SEQ ID NOs: 182-184 and 186-188,        and wherein the mutant allele of the endogenous GA20 oxidase_3        locus produces a RNA transcript comprising the antisense RNA        sequence.    -   47. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a DNA        segment inserted into the endogenous GA20 oxidase_5 locus,        wherein the DNA segment encodes an antisense RNA sequence that        is at least 70% complementary to at least 20 consecutive        nucleotides of one or more of SEQ ID NOs: 182-184 and 186-188,        and wherein the mutant allele of the endogenous GA20 oxidase_5        locus produces a RNA transcript comprising the antisense RNA        sequence.    -   48. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of an endogenous        Brachytic2 (br2) locus, wherein the mutant allele comprises a        DNA segment inserted into the endogenous br2 locus, wherein the        DNA segment encodes an antisense RNA that is at least 70%        complementary to at least 20 consecutive nucleotides of SEQ ID        NO: 132 or 180, and wherein the mutant allele of the endogenous        br2 locus produces an RNA transcript comprising the antisense        RNA sequence.    -   49. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of an endogenous        Brachytic2 (br2) locus, wherein the mutant allele comprises a        deletion of at least one nucleotide from an endogenous br2 locus        as compared to SEQ ID NO: 132.    -   50. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a dominant or semi-dominant transgene        or mutant allele of a gene, and wherein the transgene or mutant        allele causes a short stature phenotype in the at least one corn        plant.    -   51. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a premature stop codon within a nucleic        acid sequence encoding a Brachytic2 protein as compared to a        control corn plant.    -   52. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of the        transcription termination sequence of the endogenous Zm.SAMT        gene, and wherein the mutant allele produces a RNA molecule        comprising an antisense sequence complementary to all or part of        the sense strand of the endogenous GA20 oxidase_5 gene.    -   53. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of the        intergenic region between the endogenous GA20 oxidase_5 and        Zm.SAMT genes, and wherein the mutant allele produces a RNA        molecule comprising an antisense sequence complementary to all        or part of the sense strand of the endogenous GA20 oxidase_5        gene.    -   54. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of one        or more of the following: 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion        thereof, and the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)        exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon,        4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)        intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any        portion thereof, of the endogenous Zm.SAMT gene.    -   55. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification which results in the transcription of an antisense        strand of at least an exon, an intron, or an untranslated region        (UTR) of the endogenous GA20 oxidase_5 gene, or any portion        thereof.    -   56. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises the Zm.SAMT        gene promoter, or a functional part thereof, operably linked to        at least one transcribable antisense sequence of at least an        exon, intron or untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof    -   57. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a sequence        selected from the group consisting of SEQ ID NOs: 87-105.    -   58. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a first        sequence and a second sequence; wherein the first sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any        complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any complementary sequence thereof, and        any portion of the foregoing, of the endogenous Zm.SAMT gene;        wherein the first sequence and the second sequence are        contiguous or separated only by an intervening sequence of fewer        than 555 nucleotides.    -   59. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genomic        deletion relative to a wild type allele of the endogenous GA20        oxidase_5 locus, wherein the genomic deletion is flanked by a        first sequence and a second sequence; wherein the first sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any        complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any complementary sequence thereof, and        any portion of the foregoing, of the endogenous Zm.SAMT gene.    -   60. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genomic        sequence comprising a first sequence and a second sequence;        wherein the first sequence comprises at least 15 consecutive        nucleotides of one or more of SEQ ID NOs: 228-235 and 276-283;        wherein the second sequence comprises at least 15 consecutive        nucleotides of one or more of SEQ ID NOs: 235-276; and wherein        the genomic sequence is at least 50 consecutive nucleotides in        length, and/or fewer than 9000 consecutive nucleotides in        length.    -   61. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_3 locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous GA20        oxidase_3 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_3 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   62. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous GA20        oxidase_5 locus, wherein the DNA segment encodes an antisense        RNA sequence that is at least 70% complementary to at least 20        consecutive nucleotides of one or more of SEQ ID NOs: 182-184        and 186-188, and wherein the mutant allele of the endogenous        GA20 oxidase_5 locus produces a RNA transcript comprising the        antisense RNA sequence.    -   63. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of an endogenous Brachytic2 (br2) locus, wherein the mutant        allele comprises a DNA segment inserted into the endogenous br2        locus, wherein the DNA segment encodes an antisense RNA that is        at least 70% complementary to at least 20 consecutive        nucleotides of SEQ ID NO: 132 or 180, and wherein the mutant        allele of the endogenous br2 locus produces an RNA transcript        comprising the antisense RNA sequence.    -   64. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of an endogenous Brachytic2 (br2) locus, wherein the mutant        allele comprises a deletion of at least one nucleotide from an        endogenous br2 locus as compared to SEQ ID NO: 132.    -   65. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a dominant or        semi-dominant transgene or mutant allele of a gene, and wherein        the transgene or mutant allele causes a short stature phenotype        in the at least one corn plant.    -   66. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a premature        stop codon within a nucleic acid sequence encoding a Brachytic2        protein as compared to a control corn plant.    -   67. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of the transcription termination sequence of        the endogenous Zm.SAMT gene, and wherein the mutant allele        produces a RNA molecule comprising an antisense sequence        complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   68. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of the intergenic region between the        endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the        mutant allele produces a RNA molecule comprising an antisense        sequence complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   69. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification comprising a deletion of        at least a portion of one or more of the following: 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any portion thereof, of the endogenous        Zm.SAMT gene.    -   70. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genome modification which results in the        transcription of an antisense strand of at least an exon, an        intron, or an untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof.    -   71. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises the Zm.SAMT gene promoter, or a functional part        thereof, operably linked to at least one transcribable antisense        sequence of at least an exon, intron or untranslated region        (UTR) of the endogenous GA20 oxidase_5 gene, or any portion        thereof.    -   72. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a sequence selected from the group consisting        of SEQ ID NOs: 87-105.    -   73. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a first sequence and a second sequence; wherein        the first sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′        UTR, and any complementary sequence thereof, and any portion of        the foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and        wherein the second sequence comprises one or more of the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,        5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th)        intron, 8^(th) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.SAMT gene; wherein the first sequence and the second sequence        are contiguous or separated only by an intervening sequence of        fewer than 555 nucleotides.    -   74. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genomic deletion relative to a wild type        allele of the endogenous GA20 oxidase_5 locus, wherein the        genomic deletion is flanked by a first sequence and a second        sequence; wherein the first sequence comprises one or more of        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.GA20 oxidase_5 gene; and wherein the second sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)        exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon,        6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR,        and any complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.SAMT gene.    -   75. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a mutant allele        of the endogenous GA20 oxidase_5 locus, wherein the mutant        allele comprises a genomic sequence comprising a first sequence        and a second sequence; wherein the first sequence comprises at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        228-235 and 276-283; wherein the second sequence comprises at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        235-276; and wherein the genomic sequence is at least 50        consecutive nucleotides in length, and/or fewer than 9000        consecutive nucleotides in length.    -   76. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_3 locus, wherein the mutant allele comprises a DNA        segment inserted into the endogenous GA20 oxidase_3 locus,        wherein the DNA segment encodes an antisense RNA sequence that        is at least 70% complementary to at least 20 consecutive        nucleotides of one or more of SEQ ID NOs: 182-184 and 186-188,        and wherein the mutant allele of the endogenous GA20 oxidase_3        locus produces a RNA transcript comprising the antisense RNA        sequence.    -   77. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a DNA        segment inserted into the endogenous GA20 oxidase_5 locus,        wherein the DNA segment encodes an antisense RNA sequence that        is at least 70% complementary to at least 20 consecutive        nucleotides of one or more of SEQ ID NOs: 182-184 and 186-188,        and wherein the mutant allele of the endogenous GA20 oxidase_5        locus produces a RNA transcript comprising the antisense RNA        sequence.    -   78. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of an endogenous        Brachytic2 (br2) locus, wherein the mutant allele comprises a        DNA segment inserted into the endogenous br2 locus, wherein the        DNA segment encodes an antisense RNA that is at least 70%        complementary to at least 20 consecutive nucleotides of SEQ ID        NO: 132 or 180, and wherein the mutant allele of the endogenous        br2 locus produces an RNA transcript comprising the antisense        RNA sequence.    -   79. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of an endogenous        Brachytic2 (br2) locus, wherein the mutant allele comprises a        deletion of at least one nucleotide from an endogenous br2 locus        as compared to SEQ ID NO: 132.    -   80. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a dominant or semi-dominant transgene        or mutant allele of a gene, and wherein the transgene or mutant        allele causes a short stature phenotype in the at least one corn        plant.    -   81. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a premature stop codon within a nucleic        acid sequence encoding a Brachytic2 protein as compared to a        control corn plant.    -   82. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of the        transcription termination sequence of the endogenous Zm.SAMT        gene, and wherein the mutant allele produces a RNA molecule        comprising an antisense sequence complementary to all or part of        the sense strand of the endogenous GA20 oxidase_5 gene.    -   83. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of the        intergenic region between the endogenous GA20 oxidase_5 and        Zm.SAMT genes, and wherein the mutant allele produces a RNA        molecule comprising an antisense sequence complementary to all        or part of the sense strand of the endogenous GA20 oxidase_5        gene.    -   84. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification comprising a deletion of at least a portion of one        or more of the following: 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion        thereof, and the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)        exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon,        4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)        intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any        portion thereof, of the endogenous Zm.SAMT gene.    -   85. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genome        modification which results in the transcription of an antisense        strand of at least an exon, an intron, or an untranslated region        (UTR) of the endogenous GA20 oxidase_5 gene, or any portion        thereof.    -   86. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises the Zm.SAMT        gene promoter, or a functional part thereof, operably linked to        at least one transcribable antisense sequence of at least an        exon, intron or untranslated region (UTR) of the endogenous GA20        oxidase_5 gene, or any portion thereof    -   87. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a sequence        selected from the group consisting of SEQ ID NOs: 87-105.    -   88. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a first        sequence and a second sequence; wherein the first sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any        complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any complementary sequence thereof, and        any portion of the foregoing, of the endogenous Zm.SAMT gene;        wherein the first sequence and the second sequence are        contiguous or separated only by an intervening sequence of fewer        than 555 nucleotides.    -   89. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genomic        deletion relative to a wild type allele of the endogenous GA20        oxidase_5 locus, wherein the genomic deletion is flanked by a        first sequence and a second sequence; wherein the first sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any        complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein        the second sequence comprises one or more of the 5′ UTR, 1^(st)        exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,        3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th)        intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,        8^(th) exon, 3′ UTR, and any complementary sequence thereof, and        any portion of the foregoing, of the endogenous Zm.SAMT gene.    -   90. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a mutant allele of the endogenous GA20        oxidase_5 locus, wherein the mutant allele comprises a genomic        sequence comprising a first sequence and a second sequence;        wherein the first sequence comprises at least 15 consecutive        nucleotides of one or more of SEQ ID NOs: 228-235 and 276-283;        wherein the second sequence comprises at least 15 consecutive        nucleotides of one or more of SEQ ID NOs: 235-276; and wherein        the genomic sequence is at least 50 consecutive nucleotides in        length, and/or fewer than 9000 consecutive nucleotides in        length.    -   91. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_3 locus, wherein        the mutant allele comprises a DNA segment inserted into the        endogenous GA20 oxidase_3 locus, wherein the DNA segment encodes        an antisense RNA sequence that is at least 70% complementary to        at least 20 consecutive nucleotides of one or more of SEQ ID        NOs: 182-184 and 186-188, and wherein the mutant allele of the        endogenous GA20 oxidase_3 locus produces a RNA transcript        comprising the antisense RNA sequence.    -   92. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a DNA segment inserted into the        endogenous GA20 oxidase_5 locus, wherein the DNA segment encodes        an antisense RNA sequence that is at least 70% complementary to        at least 20 consecutive nucleotides of one or more of SEQ ID        NOs: 182-184 and 186-188, and wherein the mutant allele of the        endogenous GA20 oxidase_5 locus produces a RNA transcript        comprising the antisense RNA sequence.    -   93. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of an endogenous Brachytic2 (br2) locus, wherein        the mutant allele comprises a DNA segment inserted into the        endogenous br2 locus, wherein the DNA segment encodes an        antisense RNA that is at least 70% complementary to at least 20        consecutive nucleotides of SEQ ID NO: 132 or 180, and wherein        the mutant allele of the endogenous br2 locus produces an RNA        transcript comprising the antisense RNA sequence.    -   94. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of an endogenous Brachytic2 (br2) locus, wherein        the mutant allele comprises a deletion of at least one        nucleotide from an endogenous br2 locus as compared to SEQ ID        NO: 132.    -   95. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a dominant or semi-dominant transgene or mutant allele of a        gene, and wherein the transgene or mutant allele causes a short        stature phenotype in the at least one corn plant.    -   96. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a premature stop codon within a nucleic acid sequence encoding a        Brachytic2 protein as compared to a control corn plant.    -   97. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genome modification comprising a        deletion of at least a portion of the transcription termination        sequence of the endogenous Zm.SAMT gene, and wherein the mutant        allele produces a RNA molecule comprising an antisense sequence        complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   98. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genome modification comprising a        deletion of at least a portion of the intergenic region between        the endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the        mutant allele produces a RNA molecule comprising an antisense        sequence complementary to all or part of the sense strand of the        endogenous GA20 oxidase_5 gene.    -   99. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genome modification comprising a        deletion of at least a portion of one or more of the following:        5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron,        3^(rd) exon, 3′ UTR, and any portion thereof, and the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,        5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th)        intron, 8^(th) exon, 3′ UTR, and any portion thereof, of the        endogenous Zm.SAMT gene.    -   100. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genome modification which results        in the transcription of an antisense strand of at least an exon,        an intron, or an untranslated region (UTR) of the endogenous        GA20 oxidase_5 gene, or any portion thereof.    -   101. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises the Zm.SAMT gene promoter, or a        functional part thereof, operably linked to at least one        transcribable antisense sequence of at least an exon, intron or        untranslated region (UTR) of the endogenous GA20 oxidase_5 gene,        or any portion thereof.    -   102. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a sequence selected from the group        consisting of SEQ ID NOs: 87-105.    -   103. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a first sequence and a second        sequence; wherein the first sequence comprises one or more of        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.GA20 oxidase_5 gene; and wherein the second sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)        exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon,        6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR,        and any complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.SAMT gene; wherein the first        sequence and the second sequence are contiguous or separated        only by an intervening sequence of fewer than 555 nucleotides.    -   104. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genomic deletion relative to a        wild type allele of the endogenous GA20 oxidase_5 locus, wherein        the genomic deletion is flanked by a first sequence and a second        sequence; wherein the first sequence comprises one or more of        the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)        intron, 3^(rd) exon, 3′ UTR, and any complementary sequence        thereof, and any portion of the foregoing, of the endogenous        Zm.GA20 oxidase_5 gene; and wherein the second sequence        comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron,        2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)        exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon,        6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR,        and any complementary sequence thereof, and any portion of the        foregoing, of the endogenous Zm.SAMT gene.    -   105. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a mutant allele of the endogenous GA20 oxidase_5 locus, wherein        the mutant allele comprises a genomic sequence comprising a        first sequence and a second sequence; wherein the first sequence        comprises at least 15 consecutive nucleotides of one or more of        SEQ ID NOs: 228-235 and 276-283; wherein the second sequence        comprises at least 15 consecutive nucleotides of one or more of        SEQ ID NOs: 235-276; and wherein the genomic sequence is at        least 50 consecutive nucleotides in length, and/or fewer than        9000 consecutive nucleotides in length.    -   106. The method of any one of embodiments 1-1(n), 3-Error!        Reference source not found., or 4-Error! Reference source not        found., wherein said harvesting is performed at least 55 days,        at least 60 days, at least 75 days, at least 90 days, or at        least 120 days after said fertilization or silking.    -   107. The method of any one of embodiments 2-2(n), 4-Error!        Reference source not found., or 5-6, wherein said harvesting is        performed at least 55 days, at least 60 days, at least 75 days,        at least 90 days, or at least 120 days after at least 50% of        said corn plants have reached R3 stage.    -   108. The method of any one of embodiments 1-7(n), wherein fewer        than or equal to 40%, fewer than or equal to 30%, fewer than or        equal to 20%, or fewer than or equal to 10% of said corn plants        have lodged at the time of harvest.    -   109. The method of any one of embodiments 1-105, wherein the        average height of said corn plants is less than or equal to 1.7        meters, less than or equal to 1.6 meters, or less than or equal        to 1.5 meters at the time of harvest.    -   110. The method of embodiment 109, wherein said height is        measured as the distance between the soil and the ligule of the        uppermost fully-expanded leaf.    -   111. The method of any one of embodiments 1-105, wherein at        least 50% of said corn plants are inbred corn plants.    -   112. The method of any one of embodiments 1-105, wherein at        least 50% of said corn plants are hybrid corn plants.    -   113. The method of any one of embodiments 1-105, wherein at        least 50% of said corn plants are semi-dwarf corn plants.    -   114. The method of any one of embodiments 1-105, wherein at        least 50% of said corn plants are dwarf corn plants.    -   115. The method of any one of embodiments 1-105, wherein at        least 50% of said corn plants are brachytic corn plants.    -   116. The method of any one of embodiments 1-105, wherein said        field comprises a planting density of at least 10,000 corn        plants per acre.    -   117. The method of any one of embodiments 1-105, wherein the        average yield of said field is at least 180 bushels per acre, at        least 190 bushels per acre, at least 200 bushels per acre, at        least 210 bushels per acre, at least 220 bushels per acre, or at        least 250 bushels per acre.    -   118. The method of any one of embodiments 1-90, wherein the        average kernel moisture content is equal to or less than 29%,        equal to or less than 28%, equal to or less than 27%, equal to        or less than 26%, equal to or less than 25%, equal to or less        than 24%, equal to or less than 23%, equal to or less than 22%,        equal to or less than 21%, equal to or less than 20%, equal to        or less than 19%, equal to or less than 15%, equal to or less        than 10%.    -   119. The method of any one of embodiments 1-105, wherein the        average kernel moisture content is between 10% and 20%, between        13% and 20%, between 15% and 20%, between 10% and 25%, between        13% and 25%, between 15% and 25%, between 20% and 25%, between        13% and 30%, between 15% and 30%, between 20% and 30%, or        between 25% and 30%.    -   120. The method of any one of embodiments 91-105, wherein said        harvesting occurs at least 2 days, at least 3 days, at least 4        days, at least 5 days, at least 6 days, at least 7 days, at        least 8 days, at least 9 days, at least 10 days, at least 15        days, at least 20 days, at least 25 days, at least 30 days, at        least 35 days, at least 40 days at least 45 days, at least 50        days, at least 55 days, at least 60 days, at least 65 days, or        at least 70 days after an average kernel moisture content of        said plurality of corn plants is between 10% and 30%, or the        kernel moisture content of a corn plant of the plurality of corn        plants is between 10% and 30%.    -   121. The method of any one of embodiments 1-105, wherein said        method comprises growing said plurality of said corn plants in        said corn field prior to said harvesting.    -   122. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein the mutant allele of the endogenous GA20        oxidase_3 locus suppresses the expression of a wild-type allele        of the endogenous GA20 oxidase_3 locus, a wild-type allele of        the endogenous GA20 oxidase_5 locus, or both.    -   123. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein the DNA segment comprises a nucleotide sequence        originating from the endogenous GA20 oxidase_3 locus.    -   124. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein the DNA segment corresponds to an inverted        genomic fragment of the endogenous GA20 oxidase_3 locus.    -   125. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein the DNA segment comprises a nucleotide sequence        originating from the endogenous GA20 oxidase_5 locus.    -   126. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein at least a portion of the antisense RNA sequence        is at least 70% complementary to a corresponding endogenous        sequence of the RNA transcript.    -   127. The method of embodiment 126, wherein the corresponding        endogenous sequence of the RNA transcript is at least 85%        identical to at least 20 consecutive nucleotides of one or more        of SEQ ID NOs: 182-184 and 186-188.    -   128. The method of any one of embodiments 1, 16, 31, 46, 61, 76,        and 91, wherein the DNA segment is inserted near or adjacent to        a corresponding endogenous DNA segment of the endogenous GA20        oxidase_3 locus.    -   129. The method of embodiment 128, wherein the antisense RNA        sequence forms a stem-loop structure with the corresponding        endogenous sequence of the RNA transcript.    -   130. The method of embodiment 128, wherein the inserted DNA        segment and the corresponding endogenous DNA segment of the        mutant allele are separated by an intervening DNA sequence.    -   131. The method of embodiment 130, wherein the intervening DNA        sequence comprises a native sequence of the endogenous GA20        oxidase_3 locus.    -   132. The method of embodiment 130, wherein the intervening DNA        sequence comprises an exogenous sequence inserted into the        endogenous GA20 oxidase_3 locus.    -   133. The method of embodiment 126, wherein the DNA segment is        inserted within a region selected from the group consisting of        5′ untranslated region (UTR), 1^(st) exon, 1^(st) intron, 2^(nd)        exon, 2^(nd) intron, 3^(rd) exon and 3′ UTR of the endogenous        GA20 oxidase_3 locus, and a combination thereof.    -   134. The method of embodiment 126, wherein the DNA segment is        inserted at a genomic site recognized by a targeted editing        technique to create a double-stranded break (DSB).    -   135. The method of embodiment 126, wherein the mutant allele        further comprises a deletion of at least one portion of the        endogenous GA20 oxidase_3 locus.    -   136. The method of embodiment 126, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence of the endogenous GA20 oxidase_3 or GA20        oxidase_5 locus.    -   137. The method of embodiment 126, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an untranslated region (UTR) sequence of the endogenous GA20        oxidase_3 or GA20 oxidase_5 locus.    -   138. The method of embodiment 126, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence and an intron sequence of the endogenous        GA20 oxidase_3 or GA20 oxidase_5 locus, the exon sequence and        the intron sequence being contiguous within the endogenous        locus.    -   139. The method of embodiment 126, wherein the DNA segment        comprises a sequence having at least at least 70% identity to        one or more of SEQ ID Nos: 194, 195, 207, 209, 211, 213, and        217.    -   140. The method of any one of embodiments 2, 17, 32, 47, 62, 77,        or 92, wherein the mutant allele of the endogenous GA20        oxidase_5 locus suppresses the expression of a wild-type allele        of the endogenous GA20 oxidase_3 locus, a wild-type allele of        the endogenous GA20 oxidase_5 locus, or both.    -   141. The method of any one of embodiments 2, 17, 32, 47, 62, 77,        or 92, wherein the RNA transcript further comprises one or more        sequence elements of the endogenous GA20 oxidase_5 locus        selected from the group consisting of 5′ UTR, 1^(st) exon,        1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR,        and any portion thereof    -   142. The method of any one of embodiments 2, 17, 32, 47, 62, 77,        or 92, wherein the DNA segment comprises a nucleotide sequence        originating from the endogenous GA20 oxidase_3 locus.    -   143. The method of embodiment 142, wherein the DNA segment        corresponds to an inverted genomic fragment of the endogenous        GA20 oxidase_3 locus.    -   144. The method of any one of embodiments 2, 17, 32, 47, 62, 77,        or 92, wherein the DNA segment comprises a nucleotide sequence        originating from the endogenous GA20 oxidase_5 locus.    -   145. The method of embodiment 142, wherein the DNA segment        corresponds to an inverted genomic fragment of the endogenous        GA20 oxidase_5 locus.    -   146. The method of any one of embodiments 2, 17, 32, 47, 62, 77,        or 92, wherein at least a portion of the antisense RNA sequence        is at least 70% complementary to a corresponding endogenous        sequence of the RNA transcript.    -   147. The method of embodiment 146, wherein the corresponding        endogenous sequence of the RNA transcript is at least 85%        identical to at least 20 consecutive nucleotides of one or more        of SEQ ID NOs: 182-184 and 186-188.    -   148. The method of embodiment 146, wherein the DNA segment is        inserted near or adjacent to a corresponding endogenous DNA        segment of the endogenous GA20 oxidase_5 locus.    -   149. The method of embodiment 148, wherein the antisense RNA        sequence forms a stem-loop structure with the corresponding        endogenous sequence of the RNA transcript.    -   150. The method of embodiment 148, wherein the inserted DNA        segment and the corresponding endogenous DNA segment of the        mutant allele are separated by an intervening DNA sequence.    -   151. The method of embodiment 150, wherein the intervening DNA        sequence comprises a native sequence of the endogenous GA20        oxidase_5 locus.    -   152. The method of embodiment 150, wherein the intervening DNA        sequence comprises an exogenous sequence inserted into the        endogenous GA20 oxidase_5 locus.    -   153. The method of embodiment 146, wherein the DNA segment is        inserted within a region selected from the group consisting of        5′ untranslated region (UTR), 1^(st) exon, 1^(st) intron, 2^(nd)        exon, 2^(nd) intron, 3^(rd) exon and 3′ UTR of the endogenous        GA20 oxidase_5 locus, and a combination thereof.    -   154. The method of embodiment 146, wherein the DNA segment is        inserted at a genomic site recognized by a targeted editing        technique to create a double-stranded break (DSB).    -   155. The method of embodiment 146, wherein the mutant allele        further comprises a deletion of at least one portion of the        endogenous GA20 oxidase_5 locus.    -   156. The method of embodiment 146, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence of the endogenous GA20 oxidase_3 or GA20        oxidase_5 locus.    -   157. The method of embodiment 146, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an untranslated region (UTR) sequence of the endogenous GA20        oxidase_3 or GA20 oxidase_5 locus.    -   158. The method of embodiment 146, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence and an intron sequence of the endogenous        GA20 oxidase_3 or GA20 oxidase_5 locus, the exon sequence and        the intron sequence being contiguous within the endogenous        locus.    -   159. The method of embodiment 146, wherein the DNA segment        comprises a sequence having at least at least 70% identity to        one or more of SEQ ID Nos: 194, 195, 207, 209, 211, 213, and        217.    -   160. The method of any one of embodiments 1, 2, 16, 17, 31, 32,        46, 47, 61, 62, 76, 77, 91, or 92, wherein the level of one or        more active GAs in at least one internode tissue of the stem or        stalk of the modified corn plant is lower than the same        internode tissue of an unmodified control plant.    -   161. The method of any one of embodiments 7-9, 22-24, 37-39,        52-54, 67-69, 82-84, or 97-99, wherein the mutant allele        comprises the endogenous Zm.SAMT gene promoter, or a portion        thereof, operably linked to a transcribable DNA sequence        encoding a RNA molecule that causes suppression of one or both        of the endogenous GA20 oxidase_3 gene and the endogenous GA20        oxidase_5 gene.    -   162. The method of any one of embodiments 7-9, 22-24, 37-39,        52-54, 67-69, 82-84, or 97-99, wherein the mutant allele        comprises the endogenous Zm.SAMT gene promoter, or a portion        thereof, operably linked to a transcribable DNA sequence        encoding a RNA molecule comprising an antisense sequence that is        at least 80% complementary to all or part of the endogenous GA20        oxidase_3 or GA20 oxidase_5 gene.    -   163. The method of any one of embodiments 11, 26, 41, 56, 71,        86, or 101, wherein the transcribable DNA sequence is at least        80% complementary to a RNA transcript sequence, or a portion        thereof, encoded by the endogenous GA20 oxidase_3 or GA20        oxidase_5 gene.    -   164. The method of any one of embodiments 11, 26, 41, 56, 71,        86, or 101, wherein the transcribable DNA sequence is at least        80% complementary to at least 15 consecutive nucleotides of one        or more of SEQ ID NOs: 218-220, 222-224, 226, and 228-255.    -   165. The method of any one of embodiments 11, 26, 41, 56, 71,        86, or 101, wherein the transcribable DNA sequence is at least        80% complementary to at least 15 consecutive nucleotides of one        or more of SEQ ID NOs: 222-224 and 228-235.    -   166. The method of any one of embodiments 7-14, 22-29, 37-44,        52-59, 67-74, 82-89, or 97-104, wherein the genome modification        further deletes at least a portion of the transcription        termination sequence of the endogenous GA20 oxidase_5 gene.    -   167. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, or 97-105, wherein the genome modification        comprises a deletion of one or both of the transcription        termination sequences of the endogenous GA20 oxidase_5 and SAMT        genes.    -   168. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105 or 167, wherein the genome        modification comprises a deletion of at least 25 consecutive        nucleotides of the intergenic region between the endogenous GA20        oxidase_5 and SAMT genes.    -   169. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, 167, or 168, wherein the genome        modification comprises a deletion of the entire intergenic        region between the endogenous GA20 oxidase_5 and SAMT genes.    -   170. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-169, wherein the genome        modification comprises a deletion of one or more sequence        elements selected from the group consisting of the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3′ UTR, and any portion of the foregoing, of the        endogenous GA20 oxidase_5 gene.    -   171. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-170, wherein the genome        modification comprises a deletion of one or more sequence        elements selected from the group consisting of the 5′ UTR,        1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)        exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,        5^(th)intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th)        intron, 8^(th) exon, 3′ UTR, and any portion of the foregoing,        of the endogenous Zm.SAMT locus.    -   172. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-171, wherein the mutant        allele produces a RNA molecule comprising an antisense sequence        that is at least 80% complementary to a RNA transcript sequence,        or a portion thereof, encoded by the endogenous GA20 oxidase_5        gene.    -   173. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-172, wherein the RNA        transcript sequence comprises a sequence that is at least 90%        identical to at least 15 consecutive nucleotides of one or more        of SEQ ID NOs: 218-220, 222-224, 226, and 228-255.    -   174. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-173, wherein the RNA        transcript sequence comprises a sequence that is at least 90        identical to at least 15 consecutive nucleotides of one or more        of SEQ ID NOs: 222-224 and 228-235.    -   175. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-174, wherein the antisense        sequence of the RNA molecule is at least 80% complementary to at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        218-220, 222-224, 226, and 228-255.    -   176. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-175, wherein the antisense        sequence of the RNA molecule is at least 80% complementary to at        least 15 consecutive nucleotides of one or more of SEQ ID NOs:        222-224 and 228-235.    -   177. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-176, wherein the genome        modification results in the production of an RNA molecule        comprising an antisense sequence from a genomic segment of        selected from the group consisting of an exon, a portion of an        exon, an intron, a portion of an intron, a 5′ or 3′ untranslated        region (UTR), a portion of an UTR, and any combination of the        foregoing, of the endogenous GA20 oxidase_5 locus.    -   178. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-177, wherein the antisense        sequence can hybridize with an RNA transcript encoded by a        wild-type allele of one or both of the endogenous GA20 oxidase_3        gene and the endogenous GA20 oxidase_5 gene.    -   179. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-178, wherein the antisense        sequence can hybridize with a sense RNA transcript encoded by an        endogenous GA20 oxidase_5 gene.    -   180. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-179, wherein the antisense        sequence can hybridize with a sense RNA transcript encoded by        the mutant allele of the endogenous GA20 oxidase_5 gene.    -   181. The method of embodiment 179 or 180, wherein the sense RNA        transcript encoded by the mutant allele of the endogenous GA20        oxidase_5 gene is shortened or truncated relative to a wild-type        allele of the endogenous GA20 oxidase_5 gene.    -   182. The method of any one of embodiments 178-181, wherein the        hybridization can cause suppression of a wild-type or mutant        allele of the endogenous GA20 oxidase_3 gene, a wild-type or        mutant allele of the endogenous GA20 oxidase_5 gene, or a        wild-type or mutant allele of both genes.    -   183. The method of any one of embodiments 7-15, 22-30, 37-45,        52-60, 67-75, 82-90, 97-105, or 167-182, wherein the genome        modification comprises two or more, three or more, four or more,        five or more, or six or more non-contiguous deletions.    -   184. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the mutant allele of the endogenous br2 locus        suppresses the expression of a wild-type allele of the        endogenous br2 locus.    -   185. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the mutant allele product of the endogenous br2        locus disrupts the function of a wild-type allele product of the        endogenous br2 locus.    -   186. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the RNA transcript further comprises one or more        sequence elements of the endogenous br2 locus selected from the        group consisting of 5′UTR, first exon, first intron, second        exon, second intron, third exon, third intron, fourth exon,        fourth intron, fifth exon, 3′ UTR, and any portion thereof.    -   187. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the DNA segment comprises a nucleotide sequence        originating from the endogenous br2 locus.    -   188. The method of embodiment 187, wherein the DNA segment        corresponds to an inverted genomic fragment of the endogenous        br2 locus.    -   189. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein at least a portion of the antisense RNA sequence        is at least 70% complementary to a corresponding endogenous        sequence of the RNA transcript.    -   190. The method of embodiment 189, wherein the corresponding        endogenous sequence of the RNA transcript is at least 85%        identical to at least 20 consecutive nucleotides of SEQ ID NO:        132 or 180.    -   191. The method of embodiment 189 or 190, wherein the antisense        RNA sequence hybridizes to the corresponding endogenous sequence        of the RNA transcript.    -   192. The method of any one of embodiments 189-191, wherein the        DNA segment is inserted near or adjacent to a corresponding        endogenous DNA segment of the endogenous br2 locus.    -   193. The method of embodiment 192, wherein the antisense RNA        sequence encoded by the inserted DNA segment hybridizes to a        corresponding endogenous sequence of the RNA transcript encoded        by the corresponding endogenous DNA segment.    -   194. The method of embodiment 192 or 193, wherein the antisense        RNA sequence forms a stem-loop structure with the corresponding        endogenous sequence of the RNA transcript.    -   195. The method of embodiment 192, wherein the inserted DNA        segment and the corresponding endogenous DNA segment of the        mutant allele are separated by an intervening DNA sequence.    -   196. The method of embodiment 195, wherein the intervening DNA        sequence has a length of at least 2 consecutive nucleotides.    -   197. The method of embodiment 195, wherein the DNA segment and        the corresponding endogenous DNA segment are separated by an        intervening sequence of at most 4000 consecutive nucleotides.    -   198. The method of any one of embodiments 195-197, wherein the        intervening DNA sequence encodes an intervening RNA sequence        between the antisense RNA sequence and the corresponding        endogenous sequence of the RNA transcript.    -   199. The method of embodiment 198, wherein the RNA transcript        forms a stem-loop secondary structure with the intervening RNA        sequence forming the loop portion of the stem-loop secondary        structure.    -   200. The method of embodiment 199, wherein the stem-loop        secondary structure comprises a near-perfect-complement stem        with mismatches.    -   201. The method of embodiment 199, wherein the stem-loop        secondary structure comprises a perfect-complement stem with no        mismatch.    -   202. The method of any one of embodiments 195-201, wherein the        intervening DNA sequence comprises a native sequence of the        endogenous br2 locus.    -   203. The method of any one of embodiments 195-201, wherein the        intervening DNA sequence comprises an exogenous sequence        inserted into the endogenous br2 locus.    -   204. The method of any one of embodiments 195-201, wherein the        intervening DNA sequence comprises an intron sequence.    -   205. The method of any one of embodiments 195-201, wherein the        intervening DNA sequence does not comprise an intron sequence.    -   206. The method of claim 192, wherein the inserted DNA segment        is located upstream of the corresponding endogenous DNA segment.    -   207. The method of embodiment 192, wherein the inserted DNA        segment is located downstream of the corresponding endogenous        DNA segment.    -   208. The method of embodiment 189, wherein the DNA segment is        inserted within a region selected from the group consisting of        5′ untranslated region (UTR), first exon, first intron, second        exon, second intron, third exon, third intron, fourth exon,        fourth intron, fifth exon, and 3′ UTR of the endogenous br2        locus, and a combination thereof.    -   209. The method of embodiment 189, wherein the mutant allele        further comprises a deletion of at least one portion of the        endogenous br2 locus.    -   210. The method of embodiment 189, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence of the endogenous br2 locus.    -   211. The method of embodiment 189, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an untranslated region (UTR) sequence of the endogenous br2        locus.    -   212. The method of embodiment 189, wherein the sense strand of        the DNA segment comprises a sequence at least 70% complementary        to an exon sequence and an intron sequence of the endogenous br2        locus, the exon sequence and the intron sequence being        contiguous within the endogenous locus.    -   213. The method of embodiment 189, wherein the DNA segment        comprises a sequence having at least at least 70% identity to        one or more of SEQ ID Nos: 132 and 180.    -   214. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the DNA segment has a length of at least 15        nucleotides.    -   215. The method of any one of embodiments 3, 18, 33, 48, 63, 78,        or 93, wherein the DNA segment has a length of at most 1000        nucleotides.    -   216. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        or 94, wherein the deletion further comprises the deletion of        the at least one exon of the endogenous br2 locus as compared to        SEQ ID NO: 132.    -   217. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        or 94, wherein the deletion further comprises the deletion of        the endogenous br2 locus.    -   218. The method of embodiment 216, wherein the at least one exon        is the first exon of the endogenous br2 locus.    -   219. The method of embodiment 216, wherein the at least one exon        is the second exon of the endogenous br2 locus.    -   220. The method of embodiment 216, wherein the at least one exon        is the third exon of the endogenous br2 locus.    -   221. The method of embodiment 216, wherein the at least one exon        is the fourth exon of the endogenous br2 locus.    -   222. The method of embodiment 216, wherein the at least one exon        is the fifth exon of the endogenous br2 locus.    -   223. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        94, or 216-222, wherein the deletion further comprises a        deletion of at least one nucleotide from at least one intron of        the endogenous br2 locus.    -   224. The method of embodiment 222, wherein the deletion        comprises the deletion of the at least one intron.    -   225. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        94, or 216-224, wherein the deletion further comprises the        deletion of at least one nucleotide of the 5′-untranslated        region of the endogenous br2 locus.    -   226. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        or 94 or 216-225, wherein the deletion further comprises the        deletion of at least one nucleotide of the 3′-untranslated        region of the endogenous br2 locus.    -   227. The method of embodiment 216, wherein the deletion further        comprises the deletion of a second exon of the endogenous br2        locus.    -   228. The method of embodiment 227, wherein the two exons are        contiguous exons.    -   229. The method of embodiment 227, wherein the two exons are not        contiguous exons.    -   230. The method of claim any one of embodiments 4, 19, 34, 49,        64, 79, or 94, wherein the mutant allele encodes a truncated        protein as compared to SEQ ID NO: 181.    -   231. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        or 94 or 218-229, wherein the deletion comprises between 10        nucleotides and 8000 nucleotides.    -   232. The method of any one of embodiments 4, 19, 34, 49, 64, 79,        or 94, wherein the mutant allele encodes an mRNA transcript        comprising a premature stop codon as compared to SEQ ID NO: 180.    -   233. The method of any one of embodiments 6, 21, 36, 51, 66, 81,        or 96, wherein a protein encoded by the nucleic acid sequence is        truncated as compared to SEQ ID NO: 181.    -   234. The method of any one of embodiments 6, 21, 36, 51, 66, 81,        or 96, wherein a protein encoded by the nucleic acid sequence        comprises 1378 or fewer amino acids.    -   235. The method of any one of embodiments 6, 21, 36, 51, 66, 81,        or 96, wherein the premature stop codon is present in a region        of the nucleic acid sequence selected from the group consisting        of the first exon, the second exon, the third exon, the fourth        exon, and the fifth exon.    -   236. The method of any one of embodiments 6, 21, 36, 51, 66, 81,        96, or 233-235, wherein the premature stop codon results from a        nonsense mutation.    -   237. The method of any one of embodiments 6, 21, 36, 51, 66, 81,        96, or 233-235, wherein the premature stop codon results from a        missense mutation.    -   238. The method of any one of embodiments 5, 20, 35, 50, 65, 80,        or 95, wherein the gene is a GA20 oxidase gene.    -   239. The method of embodiment 238, wherein the GA20 oxidase gene        is a GA20 oxidase_3 gene.    -   240. The method of embodiment 238, wherein the GA20 oxidase is a        GA20 oxidase_5 gene.    -   241. The method of any one of embodiments 5, 20, 35, 50, 65, 80,        or 95, wherein the gene is a GA3 oxidase gene.    -   242. The method of any one of embodiments 5, 20, 35, 50, 65, 80,        or 95, wherein the gene is a brachytic2 gene.    -   243. The method of any one of embodiments 5, 20, 35, 50, 65, 80,        or 95, wherein the mutant allele is a dominant negative mutant        allele.    -   244. The method of embodiment 243, wherein the dominant negative        mutant allele generates an antisense RNA transcript capable of        triggering suppression of an unmodified or wildtype allele of        the gene.    -   245. The method of embodiment 243, wherein the dominant negative        mutant allele encodes a truncated protein as compared to an        unmodified allele of the gene.    -   246. The method of embodiment 243, wherein the dominant negative        mutant allele generates at least one RNA transcript capable of        forming a hairpin-loop secondary structure.    -   247. The method of embodiment 243, wherein the coding sequence        of the dominant negative mutant allele is operably linked to a        promoter of the native copy of the gene.    -   248. The method of embodiment 243, wherein the dominant negative        mutant allele comprises an inverted copy of the gene, or a        portion thereof, adjacent to a wildtype copy of the gene at the        endogenous locus of the gene.    -   249. The method of embodiment 243, wherein the dominant negative        mutant allele comprises a deletion of a portion of a chromosome        between a first region of the gene and a second region of the        gene, wherein an antisense RNA transcript of the first region of        the gene is generated following the deletion of the portion of        the chromosome.    -   250. The method of embodiment 243, wherein the dominant negative        mutant allele comprises a first promoter and a second promoter        separated by an intervening region, wherein the first promoter        and the second promoter are positioned in opposite orientations,        wherein the second promoter generates at least one antisense RNA        transcript, and wherein expression of the gene is reduced as        compared to a control corn plant that lacks the dominant        negative mutant allele.    -   251. The method of embodiment 243, wherein the dominant negative        mutant allele comprises a tissue-specific or tissue-preferred        promoter inserted into the gene in reverse orientation as        compared to the native promoter of the gene, wherein the        tissue-specific or tissue-preferred promoter generates at least        one antisense RNA transcript, and wherein expression of the gene        is reduced as compared to a control corn plant that lacks the        dominant negative mutant allele.    -   252. The method of any one of embodiments 5, 20, 35, 50, 65, 80,        or 95, wherein the mutant allele comprises an insertion, an        inversion, or a deletion as compared to a wildtype allele of the        gene.    -   253. The method of any one of embodiments 1-252, wherein the at        least one corn plant is homozygous or biallelic for the mutant        allele or transgene.    -   254. The method of any one of embodiments 1-252, wherein the at        least one corn plant is heterozygous for the mutant allele or        transgene.    -   255. The method of any one of embodiments 1-252, wherein the at        least one corn plant has improved lodging resistance relative to        an unmodified control plant.    -   256. The method of any one of embodiments 1-252, wherein the        mutant allele is a dominant allele.    -   257. The method of any one of embodiments 1-252, wherein the        mutant allele is a semi-dominant allele.    -   258. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein fewer        than or equal to 50% of said corn plants have lodged at the time        of harvest, and wherein at least one corn plant of said        plurality of corn plants comprises a recombinant DNA construct        comprising a transcribable DNA sequence encoding a GA2 oxidase        protein and a plant-expressible promoter, wherein the        transcribable DNA sequence is operably linked to the        plant-expressible promoter.    -   259. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein fewer than or equal to 50%        of said corn plants have lodged at the time of harvest, and        wherein at least one corn plant of said plurality of corn plants        comprises a recombinant DNA construct comprising a transcribable        DNA sequence encoding a GA2 oxidase protein and a        plant-expressible promoter, wherein the transcribable DNA        sequence is operably linked to the plant-expressible promoter.    -   260. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after fertilization or silking of        at least 50% of said plurality of corn plants, wherein the        average kernel moisture content is less than or equal to 30%,        and wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a recombinant        DNA construct comprising a transcribable DNA sequence encoding a        GA2 oxidase protein and a plant-expressible promoter, wherein        the transcribable DNA sequence is operably linked to the        plant-expressible promoter.    -   261. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein average kernel moisture        content is less than or equal to 30%, and wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a recombinant DNA construct comprising        a transcribable DNA sequence encoding a GA2 oxidase protein and        a plant-expressible promoter, wherein the transcribable DNA        sequence is operably linked to the plant-expressible promoter.    -   262. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at fertilization or silking        of at least 50% of said plurality of corn plants, wherein the        average yield of said field is at least 170 bushels per acre,        wherein fewer than or equal to 50% of said corn plants have        lodged at the time of harvest, and wherein at least one corn        plant of said plurality of corn plants comprises a recombinant        DNA construct comprising a transcribable DNA sequence encoding a        GA2 oxidase protein and a plant-expressible promoter, wherein        the transcribable DNA sequence is operably linked to the        plant-expressible promoter.    -   263. A method comprising harvesting a plurality of corn plants        from a field at least 50 days after at least 50% of said corn        plants have reached R3 stage, wherein the average yield of said        field is at least 170 bushels per acre, wherein fewer than or        equal to 50% of said corn plants have lodged at the time of        harvest, and wherein at least one corn plant of said plurality        of corn plants comprises a recombinant DNA construct comprising        a transcribable DNA sequence encoding a GA2 oxidase protein and        a plant-expressible promoter, wherein the transcribable DNA        sequence is operably linked to the plant-expressible promoter.    -   264. A method comprising harvesting a plurality of corn plants        from a field at least 1 day after the average kernel moisture        content of at least 50% of said plurality of corn plants is        between 10% and 30%, wherein fewer than or equal to 50% of said        corn plants have lodged at the time of harvest, and wherein at        least one corn plant of said plurality of corn plants comprises        a recombinant DNA construct comprising a transcribable DNA        sequence encoding a GA2 oxidase protein and a plant-expressible        promoter, wherein the transcribable DNA sequence is operably        linked to the plant-expressible promoter.    -   265. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80% identical to one or more of SEQ ID NOs: 325, 327, 329,        331, 333, 335, 337, 339, 341, 343, 345, 347, and 349.    -   266. The method of any one of embodiments 258-265, wherein the        transcribable DNA sequence is, or comprises a sequence that is,        at least 80% identical to one or more of SEQ ID NOs: 324, 326,        328, 330, 332, 334, 336, 338, 340, 342, 344, 346, and 348.    -   267. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 351, 353, 355,        357, 359, 361, 363, 365, 367, and/or 369.    -   268. The method of any one of embodiments 258-264 or 267,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 350, 352, 354, 356, 358, 360, 362, 364, 366, and/or 368.    -   269. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 371, 373, 375,        377, 379, 381, 383, and/or 385.    -   270. The method of any one of embodiments 258-264 or 269,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 370, 372, 374, 376, 378, 380, 382, and/or 384.    -   271. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 387, 389, 391,        393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415,        and/or 417.    -   272. The method of any one of embodiments 258-264 or 271,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408,        410, 412, 414, and/or 416.    -   273. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 419, 421, 423,        425, 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, and/or        447.    -   274. The method of any one of embodiments 258-264 or 273,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 418, 420, 421, 424, 426, 428, 430, 432, 434, 436, 438, 440,        442, 444, and/or 446.    -   275. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 449, 451, 453,        455, 457, 459, 461, 463, 465, 467, and/or 469.    -   276. The method of any one of embodiments 258-264 or 275,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, and/or        468.    -   277. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 471, 473, 475,        477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499,        and/or 501.    -   278. The method of any one of embodiments 258-264 or 277,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492,        494, 496, 498, and/or 500.    -   279. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 503, 505, 507,        509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, and/or        531.    -   280. The method of any one of embodiments 258-264 or 279,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524,        526, 528, and/or 530.    -   281. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 533, 535, 537,        539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, and/or        561.    -   282. The method of any one of embodiments 258-264 or 281,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554,        556, 558, and/or 560.    -   283. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 563, 565, 567,        569, 571, 573, and/or 575.    -   284. The method of any one of embodiments 258-264 or 283,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 562, 564, 566, 568, 570, 572, and/or 574.    -   285. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 577, 579, 581,        583, 585, 587, and/or 589.    -   286. The method of any one of embodiments 258-264 or 285,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 576, 578, 580, 582, 584, 586, and/or 588.    -   287. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 591, 593, 595,        597, 599, 601, 603, 605, and/or 607.    -   288. The method of any one of embodiments 258-264 or 287,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 590, 592, 594, 596, 598, 600, 602, 604, and/or 606.    -   289. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 609, 611, 613,        and/or 615.    -   290. The method of any one of embodiments 258-264 or 289,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 608, 610, 612, and/or 614.    -   291. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 617, 619, 621,        623, 625, 627, 629, and/or 631.    -   292. The method of any one of embodiments 258-264 or 291,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 616, 618, 620, 622, 624, 626, 628, and/or 630.    -   293. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 633, 635, 637,        639, 641, 643, and/or 645.    -   294. The method of any one of embodiments 258-264 or 293,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 632, 634, 636, 638, 640, 642, and/or 644.    -   295. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 647 and/or 649.    -   296. The method of any one of embodiments 258-264 or 295,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 646 and/or 648.    -   297. The method of any one of embodiments 258-264, wherein the        GA2 oxidase protein is, or comprises a sequence that is, at        least 80%, at least 85%, at least 90%, at least 95%, at least        96%, at least 97%, at least 98%, at least 99%, at least 99.5%,        or 100% identical to one or more of SEQ ID NOs: 651, 653, and/or        655.    -   298. The method of any one of embodiments 258-264 or 297,        wherein the transcribable DNA sequence is, or comprises a        sequence that is, at least 80%, at least 85%, at least 90%, at        least 95%, at least 96%, at least 97%, at least 98%, at least        99%, at least 99.5%, or 100% identical to one or more of SEQ ID        NOs: 650, 652, and/or 654.    -   299. The method of any one of embodiments 258-298, wherein the        plant-expressible promoter is a vascular promoter.    -   300. The method of embodiment 299, wherein the vascular promoter        comprises one of the following: a sucrose synthase promoter, a        sucrose transporter promoter, a Sh1 promoter, Commelina yellow        mottle virus (CoYMV) promoter, a wheat dwarf geminivirus (WDV)        large intergenic region (LIR) promoter, a maize streak        geminivirus (MSV) coat protein (CP) promoter, a rice yellow        stripe 1 (YS1)-like promoter, or a rice yellow stripe 2 (OsYSL2)        promoter.    -   301. The method of embodiment 299, wherein the vascular promoter        comprises a DNA sequence that is at least 80% identical to one        or more of SEQ ID NO: 658, SEQ ID NO: 659, SEQ ID NO: 660, SEQ        ID NO: 661, or SEQ ID NO: 662, or a functional portion thereof.    -   302. The method of any one of embodiments 258-298, wherein the        plant-expressible promoter is a rice tungro bacilliform virus        (RTBV) promoter.    -   303. The method of embodiment 302, wherein the RTBV promoter        comprises a DNA sequence that is at least 80% identical to one        or more of SEQ ID NOs: 656 and 657, or a functional portion        thereof.    -   304. The method of any one of embodiments 258-298, wherein the        plant-expressible promoter is a leaf promoter.    -   305. The method of embodiment 304, wherein the leaf promoter        comprises one or more of the following: a RuBisCO promoter, a        PPDK promoter, a FDA promoter, a Nadh-Gogat promoter, a        chlorophyll a/b binding protein gene promoter, a        phosphoenolpyruvate carboxylase (PEPC) promoter, or a Myb gene        promoter.    -   306. The method of embodiment 304, wherein the leaf promoter        comprises a DNA sequence that is at least 80% identical to one        or more of SEQ ID NO: 663, SEQ ID NO: 664, or SEQ ID NO: 665, or        a functional portion thereof.    -   307. The method of any one of embodiments 258-298, wherein the        plant expressible promoter is a constitutive promoter.    -   308. The method of embodiment 307, wherein the constitutive        promoter is selected from the group consisting of: an actin        promoter, a CaMV 35S or 19S promoter, a plant ubiquitin        promoter, a plant Gos2 promoter, a FMV promoter, a CMV promoter,        a MMV promoter, a PCLSV promoter, an Emu promoter, a tubulin        promoter, a nopaline synthase promoter, an octopine synthase        promoter, a mannopine synthase promoter, or a maize alcohol        dehydrogenase, or a functional portion thereof    -   309. The method of embodiment 307, wherein the constitutive        promoter comprises a DNA sequence that is at least 80% identical        to one or more of SEQ ID NOs: 666, SEQ ID NO: 667, SEQ ID NO:        668, SEQ ID NO: 669, SEQ ID NO: 670, SEQ ID NO: 671, SEQ ID NO:        672, SEQ ID NO: 673 or SEQ ID NO: 674, or a functional portion        thereof.    -   310. The method of any one of embodiments 258, 260, or 262,        wherein said harvesting is performed at least 55 days, at least        60 days, at least 75 days, at least 90 days, or at least 120        days after said fertilization or silking.    -   311. The method of any one of embodiments 259, 261, or 263,        wherein said harvesting is performed at least 55 days, at least        60 days, at least 75 days, at least 90 days, or at least 120        days after at least 50% of said corn plants have reached R3        stage.    -   312. The method of any one of embodiments 258-311, wherein fewer        than or equal to 40%, fewer than or equal to 30%, fewer than or        equal to 20%, or fewer than or equal to 10% of said corn plants        have lodged at the time of harvest.    -   313. The method of any one of embodiments 258-311, wherein the        average height of said corn plants is less than or equal to 1.7        meters, less than or equal to 1.6 meters, or less than or equal        to 1.5 meters at the time of harvest.    -   314. The method of claim 313, wherein said height is measured as        the distance between the soil and the ligule of the uppermost        fully-expanded leaf.    -   315. The method of any one of embodiments 258-314, wherein at        least 50% of said corn plants are inbred corn plants.    -   316. The method of any one of embodiments 258-314, wherein at        least 50% of said corn plants are hybrid corn plants.    -   317. The method of any one of embodiments 258-314, wherein at        least 50% of said corn plants are semi-dwarf corn plants.    -   318. The method of any one of embodiments 258-314, wherein at        least 50% of said corn plants are dwarf corn plants.    -   319. The method of any one of embodiments 258-314, wherein at        least 50% of said corn plants are brachytic corn plants.    -   320. The method of any one of embodiments 258-319, wherein said        field comprises a planting density of at least 10,000 corn        plants per acre.    -   321. The method of any one of embodiments 258-320, wherein the        average yield of said field is at least 180 bushels per acre, at        least 190 bushels per acre, at least 200 bushels per acre, at        least 210 bushels per acre, at least 220 bushels per acre, or at        least 250 bushels per acre.    -   322. The method of any one of embodiments 258-263, wherein the        average kernel moisture content is equal to or less than 29%,        equal to or less than 28%, equal to or less than 27%, equal to        or less than 26%, equal to or less than 25%, equal to or less        than 24%, equal to or less than 23%, equal to or less than 22%,        equal to or less than 21%, equal to or less than 20%, equal to        or less than 19%, equal to or less than 15%, equal to or less        than 10%.    -   323. The method of any one of embodiments 258-321, wherein the        average kernel moisture content is between 10% and 20%, between        13% and 20%, between 15% and 20%, between 10% and 25%, between        13% and 25%, between 15% and 25%, between 20% and 25%, between        13% and 30%, between 15% and 30%, between 20% and 30%, or        between 25% and 30%.    -   324. The method of any one of embodiments 264, wherein said        harvesting occurs at least 2 days, at least 3 days, at least 4        days, at least 5 days, at least 6 days, at least 7 days, at        least 8 days, at least 9 days, at least 10 days, at least 15        days, at least 20 days, at least 25 days, at least 30 days, at        least 35 days, at least 40 days at least 45 days, at least 50        days, at least 55 days, at least 60 days, at least 65 days, or        at least 70 days after an average kernel moisture content of        said plurality of corn plants is between 10% and 30%, or the        kernel moisture content of a corn plant of the plurality of corn        plants is between 10% and 30%.    -   325. The method of any one of embodiments 258-324, wherein said        method comprises growing said plurality of said corn plants in        said corn field prior to said harvesting.

Having described the present disclosure in detail, it will be apparentthat modifications, variations, and equivalent aspects are possiblewithout departing from the spirit and scope of the present disclosure asdescribed herein and in the appended claims. Furthermore, it should beappreciated that all examples in the present disclosure are provided asnon-limiting examples.

EXAMPLES Example 1: Semi-Dwarf Corn Improves Stalk Health for LateSeason Harvest

Three semi-dwarf (SD) and three wild-type control hybrids were plantedin 30 inch rows with a density of approximately 42,000 plants per acrein two separate locations. When hybrids reached full maturity, StalkLodging Percent (STLP) and Stalk Health Good Pith Percentage (SHGPP)were collected at two separate locations at normal (October 10^(th)) andlate (November 12^(th)) harvest dates. Hybrids were replicated twelvetimes for each harvest date. Hybrids harvested at normal harvest datehad a moisture content of approximately 25%. At late harvest date,hybrids had a moisture content of approximately 15%. STLP was collectedby counting the number of lodged plants by the total number of plantsper plot at normal harvest and at late harvest prior to combine harvest.After combine harvest, SHGPP was collected cutting the residual stalksbetween first and second node then observing the intactness of the pith.Percentage of intactness was designated for each stalk. As shown inTable 20, SD hybrids have improved stalk health as compared to WThybrids.

TABLE 20 Stalk Health of SD versus WT Control Hybrids at Normal andDelayed Harvest SHGPP STLP Normal Late Normal Late Stature Line HarvestHarvest Harvest Harvest SD Line #1 59.3 62.1 0.6 1.1 Line #2 70.3 52.62.1 3.5 Line #3 62.0 53.5 1.9 2.2 Semi-Dwarf Total 63.8 56.1 1.5 2.2 WTLine #4 43.5 29.4 1.5 3.4 Line #5 23.9 18.1 2.6 5.5 Line #6 51.8 49.10.5 8.6 WT Total 39.7 32.3 1.5 5.9

In this example, SD hybrids overall improved stalk health good pithpercent as compared to WT at normal harvest, 63.8 versus 39.7 percent,respectively. This improved stalk health good pith percent was observedat late harvest, 56.1 for SD hybrids compared to 32.3 for WT hybrids(FIG. 1). At late harvest, the SD hybrids significantly reduced stalklodging as compared to WT, 2.2 versus 5.9, respectively. This experimentdemonstrates that SD hybrids allow growers flexibility in harvest byextending the window of time to harvest due to improved stalk health andstandability.

Example 2: Planting Date and Corn Maturity Impacts Harvest Window

Each growing season farmers must balance planting the corn crop afterthe threat of freezing temperatures has passed, yet with enough time toallow maximum growing degree days (GDDs) for crop development tomaturity and dry down before the first frost. Corn GDDs are calculatedby subtracting the plant's lower base or threshold temperature of 50° F.(10° C.) from the average daily air temperature in ° F. or ° C. Averagedaily air temperature is calculated by averaging the daily maximum andminimum air temperatures measured in any 24-hour period. To fully maturecorn, a certain amount of accumulated GDDs are required in relation toits relative maturity (RM) and geographical location. In the northernhemisphere, including the North American continent, the corn plantingwindow is typically within the months of April, May and June (see, e.g.,FIG. 2). Emergence, growth, and pollination of the crop follows throughstages of silking, dough, dent, maturity (black layer), and grainharvest (see, e.g., FIG. 2). When corn reaches physiological maturity(black layer), it is around 30% moisture. Additionally, late-planted andfull-season corn products tend to dry more slowly. In general, it takesabout 30 GDDs per point of moisture to dry corn from maturity to 25%moisture content (see, e.g., Table 21). After reaching maturity, typicaldrying rates may range from 0.4% to 0.8% loss of moisture content perday (see, e.g., Table 21).

The optimum harvest moisture content for corn is approximately 23% to25%. At this moisture level, kernels shell easily and stalks generallystand better, which can make harvesting more efficient. A normal harvestloss level of a timely and efficient harvest can be 1% to 2%. Knowingthe grain moisture content at maturity can help predict grain moistureat different potential harvest dates. A year with wet weather and delaysin planting may result in slower field drying of corn. However, ifenough GDDs accumulate, the drying process may be hastened. Otherfactors may also come into play if harvest is delayed. For example, corncould have developed a shallow root system because of the early-seasonmoisture. In addition, conditions may have been conducive for thedevelopment of stalk rots and stalk cannibalization in corn. Thesefactors could lead to higher than normal harvest losses because of anincreased risk for stalk lodging in corn at maturity. Delaying harvestuntil corn dries down to 17% to 19% moisture content can save onartificial drying costs. However, as corn dries down in the field thereis greater potential for excess harvest losses from stalk lodging, inpart due to normal senescence of the plant through the maturationprocess. Most harvest losses are mechanical, caused by corn grain nevergetting into the combine harvester because it cannot be collected fromlodged plants on the ground. Allowing corn to dry down in the fieldcould lead to excess harvest losses, as much as 2% to 10% or more abovethe normal level from a timely and efficient harvest.

Corn plants that are less susceptible to lodging that have improvedstalk health for late season harvest reduce the risk of harvest losses(see Example 1). Farmers make planting decisions of corn hybrids basedon their geographical region and historical weather data for spring andfall frosts. In one example, a farmer in Minnesota, USA (MN) planting a100 RM corn hybrid can expect between 144 days and 149 days toaccumulate enough GDDs for crop maturity depending on the planting datebased on a 30 year average (1981-2010). The same farmer would need anadditional 9 days of GDD accumulation to reach 25% grain moisture forharvest, or 18 to 19 days of GDD accumulation to reach 20% grainmoisture for harvest (assuming optimal weather conditions). In anotherexample, a farmer in Illinois, USA (IL) planting a 110 RM corn hybridcan expect between 128 days and 138 days to accumulate enough GDDs forcrop maturity depending on the planting date based on a 30 year average(1981-2010). The same farmer would need an additional 7 to 8 days of GDDaccumulation to reach 25% grain moisture for harvest, or 15 to 16 daysof GDD accumulation to reach 20% grain moisture for harvest (assumingoptimal weather conditions). As described herein, at minimum anadditional 30 GDDs per point of grain moisture content to reach 25% orless would be required beyond the maturity date.

In the northern hemisphere, or on the North America continent, the cornharvest window is typically within the months of September and November(see, e.g., FIG. 2). The longer the amount of time from crop maturity toharvest the greater the risk of increased harvest losses unless the cornvariety can provide a benefit of improved standability (less susceptibleto lodging), such as the semi-dwarf (SD) corn in Example 1. Statedanother way, semi-dwarf or shorter stature corn varieties or lines withimproved standability that are less susceptible to lodging (such as thedisclosed SD corn) provide a benefit to farmers or crop growers byenabling grain dry down in the field for a duration of time beyondstandard expectations from conventional corn varieties. For example, afarmer would have an increased probability to reach 20% grain moistureprior to harvest, ranging from an extra 8 to 10 days at minimum (beyond25% grain moisture) for the crop to remain in the field in the exampleprovided (see, e.g., Table 21).

TABLE 2 Total Days to Seed Days Harvest Days to Harvest GDD Maturityreach Average Moisture to reach Date reach Date to Planting (Black TotalGDD at 25% 25% 20% 20% Location Year(s) RM Maturity Date Layer) GDD perday Maturity Moisture Moisture Moisture Moisture MN 1981-2010 100 2401Apr 20th Sep 16th 149 16 30% 9 Sep 25th 19 Oct 4th MN 1981-2010 100 2401May 1st Sep 22nd 144 17 30% 9 Sep 30th 18 Oct 9th MN 1981-2010 100 2401May 20th Oct 15th 148 16 30% 9 Oct 24th 18 Nov 2nd IL 1981-2010 110 2642Apr 15th Aug 31st 138 19 30% 8 Sep 7th 16 Sep 15th IL 1981-2010 110 2642May 1st Sep 7th 129 20 30% 7 Sep 14th 15 Sep 21st IL 1981-2010 110 2642Jun 1st Oct 7th 128 21 30% 7 Oct 14th 15 Oct 21st

1. A method comprising harvesting a plurality of corn plants from afield at least 50 days after fertilization or silking of at least 50% ofsaid plurality of corn plants, wherein fewer than or equal to 50% ofsaid corn plants have lodged at the time of harvest, and wherein atleast one corn plant of said plurality of corn plants comprises: (a) amutant allele of an endogenous GA20 oxidase_3 locus, wherein the mutantallele comprises a first DNA segment inserted into the endogenous GA20oxidase_3 locus, wherein the first DNA segment encodes an antisense RNAsequence that is at least 70% complementary to at least 20 consecutivenucleotides of one or more of SEQ ID NOs: 182-184 and 186-188, andwherein the mutant allele of the endogenous GA20 oxidase_3 locusproduces a RNA transcript comprising the antisense RNA sequence; or (b)a first mutant allele of an endogenous GA20 oxidase_5 locus, wherein thefirst mutant allele of the endogenous GA20 oxidase_5 locus comprises asecond DNA segment inserted into the endogenous GA20 oxidase_5 locus,wherein the second DNA segment encodes an antisense RNA sequence that isat least 70% complementary to at least 20 consecutive nucleotides of oneor more of SEQ ID NOs: 182-184 and 186-188, and wherein the mutantallele of the endogenous GA20 oxidase_5 locus produces a RNA transcriptcomprising the antisense RNA sequence; or (c) a first mutant allele ofan endogenous Brachytic2 (br2) locus, wherein the first mutant allele ofan endogenous br2 locus comprises a third DNA segment inserted into theendogenous br2 locus, wherein the third DNA segment encodes an antisenseRNA that is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence; or (d) a second mutant allele of an endogenousbr2 locus, wherein the second mutant allele of an endogenous br2 locuscomprises a deletion of at least one nucleotide from an endogenous br2locus as compared to SEQ ID NO: 132; or (e) a dominant or semi-dominanttransgene or mutant allele of a gene, and wherein the transgene ormutant allele causes a short stature phenotype in the at least one cornplant; or (f) a premature stop codon within a nucleic acid sequenceencoding a Brachytic2 protein as compared to a control corn plant; or(g) a second mutant allele of an endogenous GA20 oxidase_5 locus,wherein the second mutant allele of the endogenous GA20 oxidase_5 locuscomprises a genome modification comprising a deletion of at least aportion of the transcription termination sequence of the endogenousZm.SAMT gene, and wherein the mutant allele produces a RNA moleculecomprising an antisense sequence complementary to all or part of thesense strand of the endogenous GA20 oxidase_5 gene; or (h) a thirdmutant allele of an endogenous GA20 oxidase_5 locus, wherein the thirdmutant allele of the endogenous GA20 oxidase_5 locus comprises a genomemodification comprising a deletion of at least a portion of theintergenic region between the endogenous GA20 oxidase_5 and Zm.SAMTgenes, and wherein the mutant allele produces a RNA molecule comprisingan antisense sequence complementary to all or part of the sense strandof the endogenous GA20 oxidase_5 gene; or (i) a genome modificationcomprising a deletion of at least a portion of one or more of thefollowing: 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd)intron, 3^(rd) exon, 3′ UTR, and any portion thereof, and the 5′ UTR,1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon,3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon, 5^(th) intron,6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′UTR, and any portion thereof, of the endogenous Zm.SAMT gene; or (j) afourth mutant allele of an endogenous GA20 oxidase_5 locus, wherein thefourth mutant allele of the endogenous GA20 oxidase_5 locus comprises agenome modification which results in the transcription of an antisensestrand of at least an exon, an intron, or an untranslated region (UTR)of the endogenous GA20 oxidase_5 gene, or any portion thereof; or (k) afifth mutant allele of an endogenous GA20 oxidase_5 locus, wherein thefifth mutant allele of the endogenous GA20 oxidase_5 locus comprises theZm.SAMT gene promoter, or a functional part thereof, operably linked toat least one transcribable antisense sequence of at least an exon,intron or untranslated region (UTR) of the endogenous GA20 oxidase_5gene, or any portion thereof; or (l) a sixth mutant allele of anendogenous GA20 oxidase_5 locus, wherein the sixth mutant allele of theendogenous GA20 oxidase_5 locus comprises a sequence selected from thegroup consisting of SEQ ID NOs: 87-105; or (m) a seventh mutant alleleof an endogenous GA20 oxidase_5 locus, wherein the seventh mutant alleleof the endogenous GA20 oxidase_5 locus comprises a first sequence and asecond sequence; wherein the first sequence comprises one or more of the5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd)exon, 3′ UTR, and any complementary sequence thereof, and any portion ofthe foregoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.SAMT gene; wherein the first sequence and the secondsequence are contiguous or separated only by an intervening sequence offewer than 555 nucleotides; or (n) an eighth mutant allele of anendogenous GA20 oxidase_5 locus, wherein the eighth mutant allele of theendogenous GA20 oxidase_5 locus comprises a genomic deletion relative toa wild type allele of the endogenous GA20 oxidase_5 locus, wherein thegenomic deletion is flanked by a first sequence and a second sequence;wherein the first sequence comprises one or more of the 5′ UTR, 1^(st)exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR,and any complementary sequence thereof, and any portion of theforegoing, of the endogenous Zm.GA20 oxidase_5 gene; and wherein thesecond sequence comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th)exon, 4^(th) intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th)intron, 7^(th) exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.SAMT gene; or (o) a genomic sequence comprising a firstsequence and a second sequence; wherein the first sequence comprises atleast 15 consecutive nucleotides of one or more of SEQ ID NOs: 228-235and 276-283; wherein the second sequence comprises at least 15consecutive nucleotides of one or more of SEQ ID NOs: 235-276; andwherein the genomic sequence is at least 50 consecutive nucleotides inlength, and/or fewer than 9000 consecutive nucleotides in length; or (p)a recombinant DNA construct comprising a transcribable DNA sequenceencoding a GA2 oxidase protein and a plant-expressible promoter, whereinthe transcribable DNA sequence is operably linked to theplant-expressible promoter.
 2. A method comprising harvesting aplurality of corn plants from a field at least 50 days after at least50% of said corn plants have reached R3 stage, wherein fewer than orequal to 50% of said corn plants have lodged at the time of harvest, andwherein at least one corn plant of said plurality of corn plantscomprises: (a) a mutant allele of an endogenous GA20 oxidase_3 locus,wherein the mutant allele comprises a first DNA segment inserted intothe endogenous GA20 oxidase_3 locus, wherein the first DNA segmentencodes an antisense RNA sequence that is at least 70% complementary toat least 20 consecutive nucleotides of one or more of SEQ ID NOs:182-184 and 186-188, and wherein the mutant allele of the endogenousGA20 oxidase_3 locus produces a RNA transcript comprising the antisenseRNA sequence; or (b) a first mutant allele of an endogenous GA20oxidase_5 locus, wherein the first mutant allele of the endogenous GA20oxidase_5 locus comprises a second DNA segment inserted into theendogenous GA20 oxidase_5 locus, wherein the second DNA segment encodesan antisense RNA sequence that is at least 70% complementary to at least20 consecutive nucleotides of one or more of SEQ ID NOs: 182-184 and186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5locus produces a RNA transcript comprising the antisense RNA sequence;or (c) a first mutant allele of an endogenous Brachytic2 (br2) locus,wherein the first mutant allele of the endogenous br2 locus comprises athird DNA segment inserted into the endogenous br2 locus, wherein thethird DNA segment encodes an antisense RNA that is at least 70%complementary to at least 20 consecutive nucleotides of SEQ ID NO: 132or 180, and wherein the mutant allele of the endogenous br2 locusproduces an RNA transcript comprising the antisense RNA sequence; or (d)a second mutant allele of an endogenous br2 locus, wherein the secondmutant allele of the endogenous br2 locus comprises a deletion of atleast one nucleotide from an endogenous br2 locus as compared to SEQ IDNO: 132; or (e) a dominant or semi-dominant transgene or mutant alleleof a gene, and wherein the transgene or mutant allele causes a shortstature phenotype in the at least one corn plant; or (f) a prematurestop codon within a nucleic acid sequence encoding a Brachytic2 proteinas compared to a control corn plant; or (g) a second mutant allele of anendogenous GA20 oxidase_5 locus, wherein the second mutant allele of theendogenous GA20 oxidase_5 locus comprises a genome modificationcomprising a deletion of at least a portion of the transcriptiontermination sequence of the endogenous Zm.SAMT gene, and wherein themutant allele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene; or (h) a third mutant allele of an endogenous GA20oxidase_5 locus, wherein the third mutant allele of the endogenous GA20oxidase_5 locus comprises a genome modification comprising a deletion ofat least a portion of the intergenic region between the endogenous GA20oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene; or (i) agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion thereof, andthe 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron,3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,8^(th) exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMTgene; or (j) a fourth mutant allele of an endogenous GA20 oxidase_5locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5locus comprises a genome modification which results in the transcriptionof an antisense strand of at least an exon, an intron, or anuntranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or anyportion thereof; or (k) a fifth mutant allele of an endogenous GA20oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functionalpart thereof, operably linked to at least one transcribable antisensesequence of at least an exon, intron or untranslated region (UTR) of theendogenous GA20 oxidase_5 gene, or any portion thereof; or (l) a sixthmutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixthmutant allele of the endogenous GA20 oxidase_5 locus comprises asequence selected from the group consisting of SEQ ID NOs: 87-105; or(m) an seventh mutant allele of an endogenous GA20 oxidase_5 locus,wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locuscomprises a first sequence and a second sequence; wherein the firstsequence comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th)intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any complementary sequencethereof, and any portion of the foregoing, of the endogenous Zm.SAMTgene; wherein the first sequence and the second sequence are contiguousor separated only by an intervening sequence of fewer than 555nucleotides; or (n) a eighth mutant allele of the endogenous GA20oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20oxidase_5 locus comprises a genomic deletion relative to a wild typeallele of the endogenous GA20 oxidase_5 locus, wherein the genomicdeletion is flanked by a first sequence and a second sequence; whereinthe first sequence comprises one or more of the 5′ UTR, 1^(st) exon,1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th)intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any complementary sequencethereof, and any portion of the foregoing, of the endogenous Zm.SAMTgene; or (o) a genomic sequence comprising a first sequence and a secondsequence; wherein the first sequence comprises at least 15 consecutivenucleotides of one or more of SEQ ID NOs: 228-235 and 276-283; whereinthe second sequence comprises at least 15 consecutive nucleotides of oneor more of SEQ ID NOs: 235-276; and wherein the genomic sequence is atleast 50 consecutive nucleotides in length, and/or fewer than 9000consecutive nucleotides in length; or (p) a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.
 3. The method ofclaim 1, wherein the average kernel moisture content is less than orequal to 30%
 4. The method of claim 2, wherein average kernel moisturecontent is less than or equal to 30%
 5. The method of claim 1, whereinthe average yield of said field is at least 170 bushels per acre.
 6. Themethod of claim 2, wherein the average yield of said field is at least170 bushels per acre.
 7. A method comprising harvesting a plurality ofcorn plants from a field at least 1 day after the average kernelmoisture content of at least 50% of said plurality of corn plants isbetween 10% and 30%, wherein fewer than or equal to 50% of said cornplants have lodged at the time of harvest, and wherein at least one cornplant of said plurality of corn plants comprises: (a) a mutant allele ofan endogenous GA20 oxidase_3 locus, wherein the mutant allele comprisesa first DNA segment inserted into the endogenous GA20 oxidase_3 locus,wherein the first DNA segment encodes an antisense RNA sequence that isat least 70% complementary to at least 20 consecutive nucleotides of oneor more of SEQ ID NOs: 182-184 and 186-188, and wherein the mutantallele of the endogenous GA20 oxidase_3 locus produces a RNA transcriptcomprising the antisense RNA sequence; or (b) a first mutant allele ofan endogenous GA20 oxidase_5 locus, wherein the first mutant allele ofthe endogenous GA20 oxidase_5 locus comprises a second DNA segmentinserted into the endogenous GA20 oxidase_5 locus, wherein the secondDNA segment encodes an antisense RNA sequence that is at least 70%complementary to at least 20 consecutive nucleotides of one or more ofSEQ ID NOs: 182-184 and 186-188, and wherein the mutant allele of theendogenous GA20 oxidase_5 locus produces a RNA transcript comprising theantisense RNA sequence; or (c) a first mutant allele of an endogenousBrachytic2 (br2) locus, wherein the first mutant allele of theendogenous br2 locus comprises a third DNA segment inserted into theendogenous br2 locus, wherein the third DNA segment encodes an antisenseRNA that is at least 70% complementary to at least 20 consecutivenucleotides of SEQ ID NO: 132 or 180, and wherein the mutant allele ofthe endogenous br2 locus produces an RNA transcript comprising theantisense RNA sequence; or (d) a second mutant allele of an endogenousbr2 locus, wherein the second mutant allele comprises a deletion of atleast one nucleotide from an endogenous br2 locus as compared to SEQ IDNO: 132; or (e) a dominant or semi-dominant transgene or mutant alleleof a gene, and wherein the transgene or mutant allele causes a shortstature phenotype in the at least one corn plant; or (f) a prematurestop codon within a nucleic acid sequence encoding a Brachytic2 proteinas compared to a control corn plant; or (g) a second mutant allele of anendogenous GA20 oxidase_5 locus, wherein the second mutant allele of theendogenous GA20 oxidase_5 locus comprises a genome modificationcomprising a deletion of at least a portion of the transcriptiontermination sequence of the endogenous Zm.SAMT gene, and wherein themutant allele produces a RNA molecule comprising an antisense sequencecomplementary to all or part of the sense strand of the endogenous GA20oxidase_5 gene; or (h) a third mutant allele of an endogenous GA20oxidase_5 locus, wherein the third mutant allele of the endogenous GA20oxidase_5 locus comprises a genome modification comprising a deletion ofat least a portion of the intergenic region between the endogenous GA20oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces aRNA molecule comprising an antisense sequence complementary to all orpart of the sense strand of the endogenous GA20 oxidase_5 gene; or (i) agenome modification comprising a deletion of at least a portion of oneor more of the following: 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and any portion thereof, andthe 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd) exon, 2^(nd) intron,3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th) intron, 5^(th) exon,5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th) exon, 7^(th) intron,8^(th) exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMTgene; or (j) a fourth mutant allele of an endogenous GA20 oxidase_5locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5locus comprises a genome modification which results in the transcriptionof an antisense strand of at least an exon, an intron, or anuntranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or anyportion thereof; or (k) a fifth mutant allele of an endogenous GA20oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functionalpart thereof, operably linked to at least one transcribable antisensesequence of at least an exon, intron or untranslated region (UTR) of theendogenous GA20 oxidase_5 gene, or any portion thereof; or (l) a sixthmutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixthmutant allele of the endogenous GA20 oxidase_5 locus comprises asequence selected from the group consisting of SEQ ID NOs: 87-105; or(m) an seventh mutant allele of an endogenous GA20 oxidase_5 locus,wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locuscomprises a first sequence and a second sequence; wherein the firstsequence comprises one or more of the 5′ UTR, 1^(st) exon, 1^(st)intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th)intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any complementary sequencethereof, and any portion of the foregoing, of the endogenous Zm.SAMTgene; wherein the first sequence and the second sequence are contiguousor separated only by an intervening sequence of fewer than 555nucleotides; or (n) an eighth mutant allele of an endogenous GA20oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20oxidase_5 locus comprises a genomic deletion relative to a wild typeallele of the endogenous GA20 oxidase_5 locus, wherein the genomicdeletion is flanked by a first sequence and a second sequence; whereinthe first sequence comprises one or more of the 5′ UTR, 1^(st) exon,1^(st) intron, 2^(nd) exon, 2^(nd) intron, 3^(rd) exon, 3′ UTR, and anycomplementary sequence thereof, and any portion of the foregoing, of theendogenous Zm.GA20 oxidase_5 gene; and wherein the second sequencecomprises one or more of the 5′ UTR, 1^(st) exon, 1^(st) intron, 2^(nd)exon, 2^(nd) intron, 3^(rd) exon, 3^(rd) intron, 4^(th) exon, 4^(th)intron, 5^(th) exon, 5^(th) intron, 6^(th) exon, 6^(th) intron, 7^(th)exon, 7^(th) intron, 8^(th) exon, 3′ UTR, and any complementary sequencethereof, and any portion of the foregoing, of the endogenous Zm.SAMTgene; or (o) a genomic sequence comprising a first sequence and a secondsequence; wherein the first sequence comprises at least 15 consecutivenucleotides of one or more of SEQ ID NOs: 228-235 and 276-283; whereinthe second sequence comprises at least 15 consecutive nucleotides of oneor more of SEQ ID NOs: 235-276; and wherein the genomic sequence is atleast 50 consecutive nucleotides in length, and/or fewer than 9000consecutive nucleotides in length; or (p) a recombinant DNA constructcomprising a transcribable DNA sequence encoding a GA2 oxidase proteinand a plant-expressible promoter, wherein the transcribable DNA sequenceis operably linked to the plant-expressible promoter.
 8. The method ofclaim 1, wherein (i) at least 50% of said corn plants are inbred cornplants or (ii) wherein at least 50% of said corn plants are hybrid cornplants.
 9. The method of claim 1, wherein (i) at least 50% of said cornplants are semi-dwarf corn plants; (ii) at least 50% of said corn plantsare dwarf corn plants; or (iii) at least 50% of said corn plants arebrachytic corn plants.
 10. The method of claim 1, wherein said fieldcomprises a planting density of at least 10,000 corn plants per acre.11. The method of claim 1, wherein (i) the mutant allele of theendogenous GA20 oxidase_3 locus suppresses the expression of a wild-typeallele of the endogenous GA20 oxidase_3 locus, a wild-type allele of theendogenous GA20 oxidase_5 locus, or both; or (ii) the first mutantallele of an endogenous GA20 oxidase_5 locus suppresses the expressionof a wild-type allele of the endogenous GA20 oxidase_3 locus, awild-type allele of the endogenous GA20 oxidase_5 locus, or both. 12.The method of claim 1, wherein the first DNA segment (i) comprises anucleotide sequence originating from the endogenous GA20 oxidase_3locus; (ii) corresponds to an inverted genomic fragment of theendogenous GA20 oxidase_3 locus; or (iii) comprises a nucleotidesequence originating from the endogenous GA20 oxidase_5 locus.
 13. Themethod of claim 1, wherein the first DNA segment comprises a sequencehaving at least at least 70% identity to one or more of SEQ ID Nos: 194,195, 207, 209, 211, 213, and
 217. 14. The method of claim 1, wherein thesecond DNA segment comprises a sequence having at least at least 70%identity to one or more of SEQ ID Nos: 194, 195, 207, 209, 211, 213, and217.
 15. The method of claim 1, wherein the level of one or more activeGAs in at least one internode tissue of the stem or stalk of themodified corn plant is lower than the same internode tissue of anunmodified control plant.
 16. The method of claim 1, wherein the secondmutant allele of an endogenous GA20 oxidase_5 gene comprises theendogenous Zm.SAMT gene promoter, or a portion thereof, operably linkedto a transcribable DNA sequence encoding: (i) a RNA molecule that causessuppression of one or both of the endogenous GA20 oxidase_3 gene and theendogenous GA20 oxidase_5 gene; (ii) a RNA molecule comprising anantisense sequence that is at least 80% complementary to all or part ofthe endogenous GA20 oxidase_3 or GA20 oxidase_5 gene; or (iii) both (i)and (ii).
 17. The method of claim 1, wherein the at least onetranscribable antisense sequence is at least 80% complementary to atleast 15 consecutive nucleotides of one or more of SEQ ID NOs: 218-220,222-224, 226, and 228-255.
 18. The method of claim 1, wherein the firstmutant allele of the endogenous br2 locus suppresses the expression of awild-type allele of the endogenous br2 locus.
 19. The method of claim 1,wherein the third DNA segment comprises a sequence having at least atleast 70% identity to one or more of SEQ ID Nos: 132 and
 180. 20. Themethod claim 1, wherein the second mutant allele of an endogenous br2locus comprises the deletion of (i) at least one nucleotide of at leastone exon of the endogenous br2 locus as compared to SEQ ID NO: 132;and/or (ii) a deletion of at least one nucleotide from at least oneintron of the endogenous br2 locus.
 21. The method of claim 1, whereinthe second mutant allele of an endogenous br2 locus encodes a truncatedprotein as compared to SEQ ID NO:
 181. 22. The method of claim 1,wherein the at least one corn plant has improved lodging resistancerelative to an unmodified control plant.
 23. The method of claim 1,wherein the GA2 oxidase protein is, or comprises a sequence that is, atleast 80% identical to one or more of SEQ ID NOs: 325, 327, 329, 331,333, 335, 337, 339, 341, 343, 345, 347, 349, 351, 353, 355, 357, 359,361, 363, 365, 367, 369, 371, 373, 375, 377, 379, 381, 383, 385, 387,389, 391, 393, 395, 397, 399, 401, 403, 405, 407, 409, 411, 413, 415,417, 419, 421, 423, 425, 427, 429, 431, 433, 435, 437, 439, 441, 443,445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471,473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499,501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527,529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555,557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583,585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611,613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639,641, 643, 645, 647, 649, 651, 653, and/or
 655. 24. The method of claim1, wherein the transcribable DNA sequence is, or comprises a sequencethat is, at least 80% identical to one or more of SEQ ID NOs: 324, 326,328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354,356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382,384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410,412, 414, 416, 418, 420, 421, 424, 426, 428, 430, 432, 434, 436, 438,440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466,468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494,496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522,524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550,552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578,580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606,608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634,636, 638, 640, 642, 644, 646, 648, 650, 652, and/or
 655. 25. The methodof claim 1, wherein the plant-expressible promoter is a (i) vascularpromoter; (ii) a leaf promoter; or (iii) a constitutive promoter. 26.The method of claim 1, wherein the plant-expressible promoter is a ricetungro bacilliform virus (RTBV) promoter.